天津农学院学报 ›› 2017, Vol. 24 ›› Issue (4): 11-14.

• 研究与简报 • 上一篇    下一篇

拟南芥生物钟突变体lhy、cca1营养生长时相转变

郭蕊, 傅钰, 龙鸿通信作者   

  1. 天津农学院 园艺园林学院,天津 300384
  • 收稿日期:2017-04-18 出版日期:2017-12-31 发布日期:2019-10-15
  • 通讯作者: 龙鸿(1964-),男,广西桂平人,教授,博士,主要从事果树资源、果树生物技术研究。E-mail: longhong@tjau.edu.cn。
  • 作者简介:郭蕊(1988-),女,河北保定人,硕士,主要从事果树生物技术。E-mail: 961286947@qq.com。

Vegetative Phase Change of Circadian Clock Mutant lhy, cca1 in Arabidopsis thaliana

GUO Rui, FU Yu, LONG HongCorresponding Author   

  1. College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300384, China
  • Received:2017-04-18 Online:2017-12-31 Published:2019-10-15

摘要: 高等植物营养生长过程中,从幼龄期到成熟期的转变称营养生长时相转变(vegetative phase change, VPC),转变过程中会伴随着营养生长状态的变化。VPC与植物自身的生物节律联系紧密,但是生物钟(circadian clock)相关基因对VPC的影响还不清楚。本研究通过观察研究拟南芥生物钟相关突变体lhy、cca1在VPC过程中的变化,来探讨LHY、CCA1基因对VPC的影响。结果表明,野生型和突变体在经历VPC时,最早出现远轴面表皮毛莲座叶叶序数、叶片基角、茎尖分生组织解剖结构特征以及miR156表达水平的不同,都说明了突变体的VPC被延迟。这种延迟与茎尖分生组织原套、原体和髓分生组织的发育迟缓相关,受miR156调控。

关键词: 营养生长时相转变, lhy, cca1, 生物钟, 拟南芥

Abstract: During the development of higher plants, the transition from juvenile phase to adult phase is referred to vegetative phase change(VPC). VPC is one of the important physiological processes in plant development, which is related to circadian clock regulation. Unfortunately, regulation of circadian clock related genes during this transition remain unknown. In this paper, we investigated characters of VPC in mutants of circadian clock-related gene, LHY and CCA1. Our results showed that onset of abaxial trichomes of rosette leaves, paraffin section of shoot apical meristems and expression of miR156 indicated that vegetative phase change in lhy, cca1 is delayed. This retardation is concomitant with affecting development of SAM(including tunica, corpus and pith meristem zone), and is regulated via miR156.

Key words: vegetative phase change, lhy, cca1, circadian clock, Arabidopsis thaliana

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