火龙果茎和昙花茎的红外光谱差异分析

天津农学院学报 ›› 2017, Vol. 24 ›› Issue (2): 30-33.

火龙果茎和昙花茎的红外光谱差异分析

李雕^1a, 杨静慧^{1b,通信作者}, 秦艳筠^1b, 黄唯子^1b, 王洪妹^1b, 杨仁杰^1a

1天津农学院 a.工程技术学院,b.园艺园林学院,天津 300384

收稿日期:2016-05-11 出版日期:2017-06-30 发布日期:2019-10-15
通讯作者: 杨静慧（1961 -）,女,甘肃兰州人,教授,博士,主要从事园艺植物栽培、抗逆生理和分子育种研究。E-mail：jinghuiyang2@aliyun.com。
作者简介:李雕（1992-）,男,云南大理人,硕士在读,主要从事农业环境与能源工程研究。E-mail：1429980690@qq.com。
基金资助:
天津市农业科技成果转化与推广项目“彩叶树优良品种繁殖、栽培技术示范与推广”（201502100）; 天津市科技成果转化及产业化推进计划项目“优质彩叶北美海棠新品种种苗的规模化繁育”（14ZXNZNC0040）

Variance of Infrared Spectra between Hylocereus undlatus and Epiphyllum oxypetalum Stem

LI Diao^1a, YANG Jing-hui^{1b,Corresponding Author}, QIN Yan-jun^1b, HUANG Wei-zi^1b, WANG Hong-mei^1b, YANG Ren-jie^1a

1. Tianjin Agricultural University, a. College of Engineering and Technology, b. College of horticulture and landscape, Tianjin 300384,China

Received:2016-05-11 Online:2017-06-30 Published:2019-10-15

摘要/Abstract

摘要： 为了比较火龙果与昙花的成分差异,以火龙果与昙花的茎为试验材料,采用红外光谱、二阶导数光谱和二维相关同步光谱技术对其成分进行了分析。结果表明：红外光谱图显示火龙果茎有5个明显的特征峰,昙花茎有4个;其中两者相同的吸收峰对应的官能团为C—O—C（脂肪烃）、C—O—C、N—H、O—H（二聚体）,不同的吸收峰为1 041 cm^-1（火龙果）、1 077 cm^-1（火龙果）、1 033 cm^-1（昙花）;667~1 866 cm^-1区间的二阶导数红外光谱图显示,火龙果茎有14个吸收峰,昙花茎有16个,955 cm^-1为火龙果茎特有的吸收峰,对应的官能团为酸酐C—O;1 520 cm^-1为昙花茎特有的吸收峰,对应的官能团为胺N—H;890~1 800 cm^-1区间的二维相关同步红外光谱图显示,火龙果茎和昙花茎的交叉峰相同,其中1 321 cm^-1与1 630 cm^-1形成了较强正交叉峰,1 046 cm^-1与1 634 cm^-1、1 049 cm^-1与1 329 cm^-1形成了较弱的正交叉峰,无负交叉峰;二维相关红外光谱图显示的自动峰位置与红外光谱的一致。

关键词: 火龙果, 昙花, 红外光谱, 二阶导数, 二维相关

Abstract: In order to explore the component difference between Hylocereus undlatus and Epiphyllum oxypetalum, fourier transform infrared spectroscopy（FTIR）, second derivative infrared spectroscopy and two-dimensional correlation infrared spectroscopy（2D-IR）were analysed with their stems. The infrared spectrogram showed that there were five absorption peaks in stem of H undlatus, and four in E. oxypetalum; The similar peaks of both represent the same functional groups which were C—O—C（aliphatic hydrocarbon）、C—O—C、N—H、O—H（dimer）. But the differences were 1 041 cm^-1 H. undlatus, 1 077 cm^-1 H. undlatus, 1 033cm^-1 E. oxypetalum. The fourteen intensities absorptionpeaks was shown in stem of H. undlatus and sixteenin E. oxypetalum according to the second derivative infrared spectroscopy in the range of 667-1 866 cm^-1. An obvious characteristic peak was 955 cm^-1 in H. undlatus corresponded to the functional group of acid anhydride C—O, and 1 520 cm^-1in E. oxypetalum corresponded to the functional group of amine N—H. There were same cross peaks between H. undlatus and E. oxypetalum,on map of 2D-IR spectroscopy in the range of 890-1 800 cm^-1especially a strong positive cross peaks in the wave number of 1 321 cm^-1and 1 630 cm^-1, weaker positive cross peaks in the wave number of 1 046 cm^-1&1 634 cm^-1, 1 049 cm^-1& 1 329 cm^-1, without negative cross peak.The positions of automatic spectrum peak was same with the fourier transform infrared spectroscopy（FTIR）on two-dimensional correlation infrared spectroscopy（2D-IR）.

Key words: Hylocereus undlatus, Epiphyllum oxypetalum, infrared spectrum, second derivative spectrometry, two-dimensional correlation spectroscopy

中图分类号:

李雕, 杨静慧, 秦艳筠, 黄唯子, 王洪妹, 杨仁杰. 火龙果茎和昙花茎的红外光谱差异分析[J]. 天津农学院学报, 2017, 24(2): 30-33.

LI Diao, YANG Jing-hui, QIN Yan-jun, HUANG Wei-zi, WANG Hong-mei, YANG Ren-jie. Variance of Infrared Spectra between Hylocereus undlatus and Epiphyllum oxypetalum Stem[J]. Journal of Tianjin Agricultural University, 2017, 24(2): 30-33.

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参考文献

[1] 黎舒. 火龙果不同品系品种植物学形态和生物学特性研究[D]. 桂林:广西大学,2014.
[2] 马媛媛,党璇,杨柳. 火龙果药学研究概况[J]. 安徽农业科学,2011,39(35):21629-21630.
[3] 邓仁菊,范建新,蔡永强.国内外火龙果研究进展及产业发展现状[J]. 贵州农业科学,2011,39(6):188-192.
[4] 高慧颖,王琦,余亚白.火龙果花的研究现状与开发前景[J]. 热带生物学报,2012,3(3):281-286.
[5] 孙广东. 昙花开花特性及环境关系研究[D]. 杨凌:西北农业科技大学,2013.
[6] Meng L Q,Tang J W,Wang Y.et al.Astragaloside IV synergizes with ferulic acid to inhibit renaltubulointerstitial fibrosis in rats with obstructive nephropathy[J]. British Journal of Pharmacology,2011,162:1805–1818.
[7] Zhu S H,Wang Y,Jin J,et al.Endoplasmic reticulum stress mediates aristolochic acid I–induced apoptosis in human renal proximal tubular epithelial cells[J]. Toxicology in Vitro,2012,26(5):663–671.
[8] Gorostizaga A,Carcía M M M S,Acquier A,et al. Modulation of albumin–induced endoplasmic reticulum stress in renal proximal tubule cells by upregulation of mapk phosphatase-1[J]. Chemico–Biological Interactions,2013,206:47-54.
[9] 张振国,邹翔,徐阳,等. 昙花总黄酮提取工艺研究[J].哈尔滨商业大学学报(自然科学版),2015,31(2):129-131.
[10] 张振国,邹翔. 昙花中槲皮素、异鼠李素和山奈酚量的测定[J]. 哈尔滨商业大学学报(自然科学版),2015,31(2):513-515.
[11] 罗小艳,郭璇华. 紫外-可见分光光度法测定火龙果花中总黄酮的含量[J]. 食品研究与开发,2014,35(23):108-111.
[12] 高慧颖,王琦. 微波辅助提取火龙果花多糖的工艺研究[J]. 福建农业学报,2014,29(9):909-912.
[13] 彭星星,陈文敏. 近红外光谱技术鉴别核桃油中掺入菜籽油、大豆油及玉米油的研究[J]. 中国粮油学报, 2015,30(12):106-112.
[14] 武晓丹. 七种不同产地仙鹤草原药材及提取物的红外光谱与二维相关红外光谱的分析与鉴定[J]. 光谱学与光谱分析,2010,30(12):3221-3227.