关键词: 鲤, 谷氨酰胺, 吞噬, 呼吸爆发, 增殖

Abstract: This experiments were conducted to evaluate the effects of glutamine on the immune responses of kidney leukocytes in common carp. Five different groups were formulated by supplementing 0,0.5,1.0,1.5,2.0 mmol/L glutamine into the basal culture medium. After the leukocytes were cultured 2,6,12,24 h,the respiratory burst activity of leukocytes were determined,respectively. The results showed that with glutamine supplemented into the culture medium,respiratory burst activity of leukocytes significantly improved and with prolongation of culture time and respiratory burst activity increased firstly（P<0.05）,and then decreased. The peak occurred in 2.0 mmol/L glutamine group at 12 h. And then,leukocyte phagocytic activity was determined after cultured for 12 h and 24 h. The phagocytic activity change trend was similar with respiratory burst activity,the highest value occurred in 1.5 mmol/L glutamine group at 24 h. And no significant differences were found in 1.0,1.5, 2.0 mmol/L glutamine group,but the killing ability of leukocytesin these three groups were significantly higher than that in basal group and 0.5 mmol/L glutamine group（P<0.05）. In vivo experiment,the average weight 37 g carp were divided into 5 groups. 0,50,100,150,200 mg/kg glutamine were injected to five different group. Then,fish was fed commercial diet for 2 weeks. Results demonstrated that with the increasing concentration of glutamine the kidney leukocyte respiratory burst and phagocytosis activity significantly increased firstly（P<0.05）,and then leveled off,while the proliferation activity kept increased significantly（P<0.05）. The results of this experiment indicate that glutamine is the effective immunomodulatory in carp leukocytes.

Key words: Cyprinuscarpio, glutamine, phagocytosis, respiratory burst, proliferation