天津农学院学报 ›› 2014, Vol. 21 ›› Issue (4): 9-12.

• 研究与简报 • 上一篇    下一篇

副猪嗜血杆菌的分离鉴定与药敏试验研究

谷禹1,2, 张东超3, 弓建芳3, 徐继鹏4, 金天明3, 李留安3, 高丰1,通信作者   

  1. 1. 吉林大学 畜牧兽医学院,长春 130062;
    2. 北辰区养殖业发展服务中心,天津 300400;
    3. 天津农学院 动物科学与医学学院,天津 300384;
    4. 武清区养殖业发展服务中心,天津 301800
  • 收稿日期:2014-09-28 出版日期:2014-12-20 发布日期:2019-10-21
  • 通讯作者: 高丰(1961-),男,吉林大安人,教授,博士,主要从事兽医病理学的教学和科研工作。E-mail:gf86661@sohu.com。
  • 作者简介:谷禹(1983-),男,天津市人,助理兽医师,学士,主要从事临床兽医诊断工作。E-mail:gu83yu83@163.com。
  • 基金资助:
    天津市高校“学科领军人才培养计划”资助项目“天津市兽医学重点学科建设”(无编号); 天津市高等学校科技发展基金项目“APP菌影载体猪巴氏杆菌基因疫苗的研究”(20060720); 天津市农业科技示范推广项目“养猪业细菌性传染病的监测及防控技术推广”(201202110); 天津市高校创新团队建设项目“兽医生物技术团队建设”(TD12-5019)

Study on Isolation and Identification of Haemophilus parasuis Strain and Its Sensitivity Tests to Drug

GU Yu1,2, ZHANG Dong-chao3, GONG Jian-fang3, XU Ji-peng4, JIN Tian-ming3, LI Liu-an3, GAO Feng1,Corresponding Author   

  1. 1. College of Animal Husbandry and Veterinary Science, Jilin University, Changchun 130062, China;
    2. Breeding Industry Development Service Center of Beichen District, Tianjin 300400, China;
    3. College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China;
    4. Breeding Industry Development Service Center of Wuqing District, Tianjin 301800, China
  • Received:2014-09-28 Online:2014-12-20 Published:2019-10-21

摘要: 副猪嗜血杆菌病是一种猪的传染病,主要引起断奶仔猪和保育猪发病。试验对天津某规模化猪场疑似副猪嗜血杆菌病猪采集病变明显的组织进行细菌分离培养、显微镜镜检、生化试验等实验室诊断,结合病猪的临床症状和病理变化特点,判定该细菌分离株为副猪嗜血杆菌。药敏试验结果显示,该株副猪嗜血杆菌对头孢噻呋、阿米卡星等药物高度敏感。

关键词: 副猪嗜血杆菌, 分离鉴定, 药敏试验

Abstract: Haemophilus parasuis disease is a kind of porcine infectious disease which mainly infects weaned piglets and nursing pigs. In this paper, the obvious lesion samples were collected from pigs with suspected disease of Haemophilus parasuis, which came from a large-scale pig farm in Tianjin. Then a series of laboratory diagnoses were carried out, such as bacterial isolation and culture, microscopic examination and biochemical tests. Combined with the clinical symptoms and pathological changes characteristics of swine, it is determined the isolated bacteria is Haemophilus parasuis. Drug sensitive test results show that the isolated Haemophilus parasuis strain is highly sensitive to ceftiofur and amikacin.

Key words: Haemophilus parasuis, isolation and identification, drug sensitive test

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