天津农学院学报 ›› 2025, Vol. 32 ›› Issue (5): 47-56.doi: 10.19640/j.cnki.jtau.2025.05.008

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斑点叉尾鮰内循环流水养殖池塘微生物多样性及理化指标相关性分析

韦红烁1, 季延斌1,2, 王洋1,2,通信作者, 夏苏东1,2, 孙学亮1,2, 宋依琳1   

  1. 1.天津农学院 水产学院,天津 300392;
    2.天津市水产生态及养殖重点实验室 天津 300392
  • 收稿日期:2023-11-10 发布日期:2025-11-12
  • 通讯作者: 王洋(1986—),女,副教授,博士,研究方向:水产微生物。E-mail:474221161@qq.com。
  • 作者简介:韦红烁(2000—),女,硕士在读,研究方向:水产微生物。E-mail:981520609@qq.com。

Microbial diversity and correlation analysis of physical and chemical indexes in internal circulating flowing water aquaculture pond of channel catfish

Wei Hongshuo1, Ji Yanbin1,2, Wang Yang1,2,Corresponding Author, Xia Sudong1,2, Sun Xueliang1,2, Song Yilin1   

  1. 1. College of Fisheries, Tianjin Agricultural University, Tianjin 300392, China;
    2. Tianjin Key Laboratory of Aqua-ecology and Aquaculture, Tianjin 300392, China
  • Received:2023-11-10 Published:2025-11-12

摘要: 为阐明7~9月斑点叉尾鮰内循环流水池塘微生物多样性的变化及与理化因子的相关性,本研究采用高通量测序法分析了天津某斑点叉尾鮰池塘环境微生态,测定养殖区和过滤区水体主要理化指标,并分析二者相关性。研究结果表明,斑点叉尾鮰养殖池塘底泥和毛刷样本微生物多样性、丰富度均高于水体,并且底泥和毛刷样本的微生物具有较高的相似性,与水体相比微生物相似性较低;放线菌门的Ilumatobacteraceae与总磷呈显著负相关(P<0.05),有作为水体中总磷含量指示的潜力;暖绳菌科与亚硝酸盐氮呈极显著正相关(P<0.01),对水体中亚硝酸盐氮有调节作用;氨氮对底泥细菌群落结构有显著影响(P<0.05)。研究结果为同类型斑点叉尾鮰养殖期间的微生态及水质调控提供了参考。

关键词: 斑点叉尾鮰, 微生物多样性, 内循环流水养殖, 水质指标

Abstract: This study aimed to clarify the changes of microbial diversity in the circulating water pond of channel catfish from July to September, and the changes of physical and chemical factors and their correlation. In the study, a high-throughput sequencing method was used to analyze the environmental microecology of a channel catfish pond in Tianjin, and the water physical and chemical indexes of the aquaculture area and filtration area were determined to analyze the correlation between them. The results showed that the microbial diversity and richness of sediment and that of brush samples in the channel catfish aquaculture pond were higher than those in the water. Among them, the microorganisms in sediment and brush samples had high similarity but low similarity with those in water. Ilumatobacteraceae of Actinobacteria had a significant negative correlation with total phosphorus, which has the potential to be used as an indicator of total phosphorus content in water(P<0.05). There was a very significant positive correlation between the Caldilineaceae family and nitrite nitrogen, and it can regulate nitrite nitrogen in the water(P<0.01). Ammonia nitrogen had a significant effect on the bacterial community structure of sediment(P<0.05). The results provide a reference for the microecology and water quality control of the same type of channel catfish during aquaculture.

Key words: channel catfish, microbial diversity, internal-circulation aquaculture, water quality index

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