复硝酚钠缓解二氯吡啶酸对油菜药害的研究

doi:10.19640/j.cnki.jtau.2025.01.004

天津农学院学报 ›› 2025, Vol. 32 ›› Issue (1): 19-24.doi: 10.19640/j.cnki.jtau.2025.01.004

复硝酚钠缓解二氯吡啶酸对油菜药害的研究

王富豪¹, 郭婧华¹, 郝培培², 程有普^{1,通信作者}

1.天津农学院园艺园林学院,天津 300392;
2.天津市绿亨化工有限公司,天津 300270

收稿日期:2023-11-20 发布日期:2025-03-04
通讯作者: 程有普（1969—）,男,教授,博士,主要从事农药残留与环境行为研究。E-mail：chengyoupu@tjau.edu.cn。
作者简介:王富豪（1996—）,男,硕士在读,主要从事除草剂药害以及缓解的研究。E-mail：wangfuhao202211@163.com。
基金资助:
国家自然科学基金（31572034）; 天津市企业科技特派员项目（19JCTPJC59000）

Study on the alleviation of phytotoxicity of clopyralid by sodium nitrophenolate to oilseed rape

Wang Fuhao¹, Guo Jinghua¹, Hao Peipei², Cheng Youpu^{1,Corresponding Author}

1. College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300392, China;
2. Tianjin Lüheng Chemical Co., Ltd., Tianjin 300270, China

Received:2023-11-20 Published:2025-03-04

摘要/Abstract

摘要： 为探究复硝酚钠（Sodium nitrophenolate）缓解二氯吡啶酸（Clopyralid）对油菜药害的作用机制,采用室内盆栽试验,油菜幼苗三叶一心时,一组喷施0（CK）、0.4、0.8（推荐剂量）、1.6、3.2、4.0 g/L二氯吡啶酸,另一组药后24 h叶面喷施9 mg/L复硝酚钠,比较两组油菜叶片的抗氧化酶活性、叶绿素及类胡萝卜素含量变化。结果表明：喷施二氯吡啶酸后,油菜幼苗的抗氧化酶（CAT、POD、SOD）活性、还原型谷胱甘肽（GSH）含量随质量浓度升高呈先升高后降低的变化趋势,二氯吡啶酸质量浓度为1.6 g/L时,CAT活性最高,质量浓度为3.2 g/L时,CAT活性升高幅度降低,叶绿素和类胡萝卜素含量与CK相比分别下降30.2%、6.0%,丙二醛（MDA）含量升高165.6%,质量浓度4.0 g/L时,POD、SOD活性、GSH含量分别比CK低11.0%、26.0%、17.7%,MDA含量升高195.5%,叶绿素和类胡萝卜素含量分别下降62.9%、23.7%;喷施复硝酚钠后,与相同质量浓度的二氯吡啶酸处理相比,油菜叶片CAT、POD、SOD活性和GSH、叶绿素、类胡萝卜素含量均有明显升高,与缓解前相比MDA含量降低8.7%~44.5%,POD活性升高7.0%~11.8%,SOD活性升高10.1%~68.7%,GSH含量升高8.5%~19.6%,叶绿素含量升高7.0%~28.9%,类胡萝卜素含量升高7.1%~62.0%。综上可知,低质量浓度的二氯吡啶酸能够提高油菜叶片抗氧化酶活性和GSH含量,从而提高油菜的抗逆性,二氯吡啶酸质量浓度过高时,油菜叶片的MDA含量明显升高,抗氧化酶活性、GSH含量、叶绿素含量、类胡萝卜素含量受到明显抑制,喷施复硝酚钠后抑制作用得到不同程度的缓解,随二氯吡啶酸质量浓度升高缓解效果减弱,表明复硝酚钠可以在一定程度上减轻二氯吡啶酸对油菜的药害。

关键词: 二氯吡啶酸, 复硝酚钠, 油菜, 缓解作用

Abstract: In order to explore the mechanism of sodium nitrophenolate alleviating the phytotoxicity of clopyralid on rape, an indoor pot experiment was carried out. When the three leaves of rape seedlings were one heart, one group was sprayed with 0（CK）, 0.4, 0.8（recommended dose）, 1.6, 3.2, 4.0 g/L clopyralid, and the other group was sprayed with 9 mg/L sodium nitrophenolate on the leaf surface 24 h after treatment. The changes of antioxidant enzyme activity, chlorophyll and carotenoid content in the leaves of the two groups were compared.The results showed that the activities of antioxidant enzymes（CAT, POD, SOD）and the content of reduced glutathione（GSH）in rape seedlings increased first and then decreased with the increase of mass concentration of clopyralid. When the mass concentration of clopyralid was 1.6 g/L, the activity of CAT was the highest. When the mass concentration was 3.2 g/L, the increase of CAT activity decreased, the contents of chlorophyll and carotenoid decreased by 30.2 % and 6.0% respectively compared with CK, and the content of malondialdehyde（MDA）increased by 165.6%, when the mass concentration was 4.0 g/L. POD, SOD activity and GSH content were 11.0%, 26.0% and 17.7% lower than CK, MDA content increased by 195.5%, chlorophyll and carotenoid content decreased by 62.9% and 23.7%, respectively.After spraying sodium nitrophenolate, the activities of CAT, POD, SOD and the contents of GSH, chlorophyll and carotenoid in rape leaves were significantly increased compared with those treated with the same concentration of clopyralid. Compared with those before remission, MDA content decreased by 8.7%-44.5%, POD activity increased by 7.0%-11.8%, SOD activity increased by 10.1%-68.7%, GSH content increased by 8.5%-19.6%, chlorophyll content increased by 7.0%-28.9%, and carotenoid content increased by 7.1% -62.0%. In summary, low mass concentration of clopyralid can increase the antioxidant enzyme activity and GSH content of rape leaves, thereby improving the stress resistance of rape. When the mass concentration of clopyralid is too high, the MDA content of rape leaves is significantly increased, and the antioxidant enzyme activity, GSH content, chlorophyll content, and carotenoid content are significantly inhibited. After spraying sodium nitrophenolate, the inhibition effect is alleviated to varying degrees. With the increase of the mass concentration of clopyralid, the mitigation effect is weakened, indicating that sodium nitrophenolate can alleviate the phytotoxicity of clopyralid to rape to a certain extent.

Key words: clopyralid, sodium nitrophenolate, rape, relieving effect

中图分类号:

TQ450.1

王富豪, 郭婧华, 郝培培, 程有普. 复硝酚钠缓解二氯吡啶酸对油菜药害的研究[J]. 天津农学院学报, 2025, 32(1): 19-24.

Wang Fuhao, Guo Jinghua, Hao Peipei, Cheng Youpu. Study on the alleviation of phytotoxicity of clopyralid by sodium nitrophenolate to oilseed rape[J]. Journal of Tianjin Agricultural University, 2025, 32(1): 19-24.

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http://xuebao.tjau.edu.cn/CN/Y2025/V32/I1/19

参考文献

[1] 严茂林,葛玮玮,张翔,等. 我国油料产业形势分析与发展对策[J]. 中国油脂,2023,48(6):8-18.
[2] 沈金雄,傅廷栋,涂金星,等. 中国油菜生产及遗传改良潜力与油菜生物柴油发展前景[J]. 华中农业大学学报,2007,26(6):894-899.
[3] OZTURK H H.Energy analysis for biodiesel production from rapeseed oil[J]. Energy Exploration Exploitation,2014,32(6):1005-1031.
[4] 孙晓玉. 麦秸、稻秸水浸液对油菜及其伴生杂草种苗生长影响的差异性研究[D]. 杨凌:西北农林科技大学,2014.
[5] 郭良芝. 油菜田草害综合治理技术研究与示范[D]. 杨凌:西北农林科技大学,2012.
[6] 江慧. 乙草胺对油菜的药害及其防治技术研究[D]. 荆州:长江大学,2015.
[7] 张文芳. 5-氨基乙酰丙酸对丙酯草醚胁迫下油菜和牛繁缕幼苗生长的影响及其调控机理[D]. 杭州:浙江大学,2008.
[8] 唐建明,王勇,方雅琴. 油菜田常用除草剂药害及规避措施[J]. 杂草科学,2010,28(1):64-66.
[9] 谷涛,李永丰,张自常,等. 杂草对激素类除草剂抗药性研究进展[J]. 植物保护,2021,47(1):15-26.
[10] 潘镭文,向春阳,丁建文,等. 外源水杨酸处理油菜幼苗对盐胁迫的生理响应[J]. 天津农学院学报,2022,29(2):22-26.
[11] 宋伟丰,韦庆慧,刘凯,等. 天然植物生长调节剂芸苔素的生物活性及应用浅析[J]. 中国农学通报,2021,37(24):97-101.
[12] 王京,梁微,王磊,等.“碧护”对苹果幼苗生长和养分积累的效应[J]. 北方园艺,2019(4):24-30.
[13] GALLINA G,CREGG B,PATTERSON E,et al.Alternative integrated weed management options for clopyralid-resistant common ragweed[J]. Horticulturae,2023,9(9):985.
[14] 苏旺苍,徐洪乐,孙兰兰,等. 不同缓解处理对甲咪唑烟酸紫花苜蓿药害的缓解效果研究[J]. 植物保护,2022,48(3):270-277.
[15] 殷万元. 多菌灵与两种植物生长调节剂联合施用对连作苹果的调控作用研究[D]. 泰安:山东农业大学,2016.
[16] 屈旭,焦禹顺,王仁汉,等. 赤霉素和复硝酚钠对辣椒种子萌发及幼苗活力的影响[J]. 中国瓜菜,2019,32(11):59-63.
[17] 努尔凯麦尔·木拉提,杨亚杰,帕尔哈提·阿布都克日木,等. 小麦叶绿素含量测定方法比较[J]. 江苏农业科学,2021,49(9):156-159.
[18] 张源一,李爱,严卓立,等. 5种薄荷对PEG模拟干旱胁迫的生理响应与耐旱性评价[J]. 天津农学院学报,2022,29(1):1-7.
[19] 周香玉,徐劲松,谢伶俐,等. 甘蓝型油菜苗期响应渍害胁迫的生理调控机制[J]. 作物学报,2024,50(4):1015-1029.
[20] 曾瑞,马骊,武军艳,等. 低温胁迫下白菜型冬油菜枯叶期早晚与抗寒性的关系[J]. 中国油料作物学报,2023,45(4):766-775.
[21] 王丹,王璐瑶,董家僖,等. 盐胁迫下水杨酸对苜蓿幼苗生理特性的影响[J]. 天津农学院学报,2022,29(3):19-22,28.
[22] 万林,李张开,李素,等. 外源独脚金内酯对油菜苗期干旱胁迫的缓解效应[J]. 中国油料作物学报,2020,42(3):461-471.
[23] 王俞茜,韩娜娜,周青云,等. 基于高光谱的夏玉米叶片SPAD值估测模型研究[J]. 天津农学院学报,2022,29(3):1-8.
[24] 丁建文,张晓辰,杨兴,等. 盐胁迫下外源赤霉素对糯玉米幼苗生理特性的影响[J].天津农学院学报,2022,29(1):37-41.
[25] 贾璐绮,向春阳,陈佩静,等. 水杨酸对盐胁迫下线型辣椒幼苗生理特性的影响[J]. 天津农学院学报,2020,27(3):39-42,48.
[26] 林瑞嫦,高玉萌,王可心,等. 芸苔素内酯对糜子苯唑·二甲钠药害的缓解作用[J]. 农业环境科学学报,2022,41(4):726-734.
[27] 郝红丹,务玲玲,李青阳,等. 不同缓解药剂对萝卜仲丁灵药害的缓解作用[J]. 农药,2015,54(8):591-596.
[28] 赵曾菁,宋奇琦,赵虎,等. 模拟霜冻条件对黄瓜幼苗生长及抗氧化酶活性的影响[J]. 南方农业学报,2018,49(4):757-762.
[29] 张庆琛,郑少萌,刘应敏,等. 干旱胁迫对冬小麦幼苗叶片光合生理特性的影响[J]. 山西农业科学,2016,44(8):1077-1082.

复硝酚钠缓解二氯吡啶酸对油菜药害的研究

Study on the alleviation of phytotoxicity of clopyralid by sodium nitrophenolate to oilseed rape

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