大孔树脂纯化黑果腺肋花楸多酚的工艺研究

doi:10.19640/j.cnki.jtau.2022.01.004

天津农学院学报 ›› 2022, Vol. 29 ›› Issue (1): 19-23.doi: 10.19640/j.cnki.jtau.2022.01.004

大孔树脂纯化黑果腺肋花楸多酚的工艺研究

董心宜^1a, 陈慧慧^1a, 张宇薇^1a, 王英超^{1a,通信作者}, 张桂霞^1b, 王瑞玺²

1.天津农学院 a.基础科学学院,b.园艺园林学院,天津 300392;
2.天津市大蒲地家庭农场,天津 301600

收稿日期:2020-10-08 出版日期:2022-03-31 发布日期:2022-04-14
通讯作者: 王英超（1981—）,女,实验师,硕士,主要从事生物化学实验教学与生物技术方面的研究。E-mail：shibiology@163.com。
作者简介:董心宜（1999—）,女,本科在读,主要从事生物制药方面的研究。E-mail：3295161874@qq.com。
基金资助:
天津市现代农业产业技术体系创新团队建设,现代产业技术体系-林果-生理生态岗位专家项目（ITTHRS2018002）; 天津市大学生创新创业训练计划项目（201910061135）

Purification of polyphenols from Aronia melanocarpa by macroporous adsorption resin

Dong Xinyi^1a, Chen Huihui^1a, ZhangYuwei^1a, Wang Yingchao^{1a,Corresponding Author}, Zhang Guixia^1b, Wang Ruixi²

1. Tianjin Agricultural University, a. College of Basic Science, b. College of Horticulture and Landscape, Tianjin 300392, China;
2. Tianjin Dapudi Family Farm, Tianjin 301600, China

Received:2020-10-08 Online:2022-03-31 Published:2022-04-14

摘要/Abstract

摘要： 以黑果腺肋花楸为原料,采用大孔树脂纯化黑果腺肋花楸中的多酚类物质。从AB-8、ADS-17、HPD-100、NKA-II 4种大孔树脂中筛选出对黑果腺肋花楸多酚吸附-解吸效果最好的一种树脂,通过单因素试验确定大孔吸附树脂纯化黑果腺肋花楸多酚的静态吸附-解吸最佳工艺条件。结果表明：AB-8大孔吸附树脂对多酚的吸附-解吸效果最好,其静态吸附-解吸最佳工艺条件为：吸附时间3 h、上样液pH=4、解吸时乙醇体积分数70%;动态吸附-解吸最佳工艺条件为：上样流速2.0 mL/min、上样量80 mL、洗脱流速1.0 mL/min、洗脱体积60 mL。在该工艺条件下,黑果腺肋花楸多酚纯度由10.80%提高到52.20%,表明AB-8大孔吸附树脂对于纯化黑果腺肋花楸多酚有较好的效果。

关键词: 黑果腺肋花楸, 多酚, 大孔树脂, 纯化

Abstract: The polyphenols in Aronia melanocarpa were purified by macroporous resin. From AB-8、ADS-17、HPD-100 and NKA-II, the best resin was selected for adsorption and desorption of polyphenols from Aronia melanocarpa. The optimum static adsorption desorption conditions of polyphenols from Aronia melanocarpa were determined by single factor experiment. The results showed that AB-8 macroporous resin had the best adsorption desorption effect on polyphenols. The optimal static adsorption desorption conditions were as follows: adsorption time was three hours, pH was four, and ethanol volume fraction was 70%; dynamic adsorption desorption process conditions were as follows: sample loading flow rate was 2.0 mL/min, sample volume was 80 mL, elution flow rate was 1.0 mL/min, and elution volume was 60 mL. Under the above conditions, the purity of polyphenols from sorbus nigricans was increased from 10.80% to 52.20%, which indicated that AB-8 macroporous resin had a good effect on the purification of polyphenols from Aronia melanocarpa.

Key words: Aronia melanocarpa, polyphenols, macroporousadsorption resin, purification

中图分类号:

TS209

董心宜, 陈慧慧, 张宇薇, 王英超, 张桂霞, 王瑞玺. 大孔树脂纯化黑果腺肋花楸多酚的工艺研究[J]. 天津农学院学报, 2022, 29(1): 19-23.

Dong Xinyi, Chen Huihui, ZhangYuwei, Wang Yingchao, Zhang Guixia, Wang Ruixi. Purification of polyphenols from Aronia melanocarpa by macroporous adsorption resin[J]. Journal of Tianjin Agricultural University, 2022, 29(1): 19-23.

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http://xuebao.tjau.edu.cn/CN/Y2022/V29/I1/19

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大孔树脂纯化黑果腺肋花楸多酚的工艺研究

Purification of polyphenols from Aronia melanocarpa by macroporous adsorption resin

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