土壤支撑培养下的‘玫瑰香’葡萄组培快繁

doi:10.19640/j.cnki.jtau.2020.01.004

Journal of Tianjin Agricultural University ›› 2020, Vol. 27 ›› Issue (1): 15-20.doi: 10.19640/j.cnki.jtau.2020.01.004

• Researches and Scientific Notes • Previous Articles Next Articles

Tissue culture and rapid propagation of Vitis vinifera ‘Muscat Hamburg’ cultured in soil supporting medium

WANG Xue-tong, CHAI Ci-jiang^{Corresponding Author}, GUO Jing, ZHU Lin, HE Xiang-su

College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300384, China

Received:2019-08-24 Published:2020-04-01

Abstract

Abstract: To get the technique of tissue culture and rapid propagation of Vitis vinifera ‘Muscat Hamburg’, the annual stem sections of ‘Muscat Hamburg’ were respectively picked up in late June and late September, sterilized with sodium hypochlorite and cultured in vitro. The sterile shoots proliferated in medium containing different concentrations of IBA, rooted in the soil-supported medium, and the transplanting with soil lump was carried out. The results showed that picking the stem sections on 27 June and soaking in 2% sodium hypochlorite for 10~15 minutes was better. In hormone-free GS medium, the propagation coefficient of test-tube plantlets could reach 3.6 but in the medium of 0.1~0.4 mg/L of IBA had no significant effect on proliferation. Two-node stem sections in the soil-supporting medium obtained the rooting rate of 84.4% which had no significant difference compared with test-tube plantlets cultured in agar medium, and the test-tube seedlings in soil-supporting medium had root hairs. The survival rate of test-tube seedlings cultured in soil supporting medium and transplanted with soil lump was 75.0% , which was significantly higher than that in agar medium. The results will provide a basis for the establishment of tissue culture and rapid propagation technology system of ‘Muscat Hamburg’.

Key words: Vitis vinifera ‘Muscat Hamburg’, tissue culture, rapid propagation, soil supporting medium

CLC Number:

WANG Xue-tong, CHAI Ci-jiang, GUO Jing, ZHU Lin, HE Xiang-su. Tissue culture and rapid propagation of Vitis vinifera ‘Muscat Hamburg’ cultured in soil supporting medium[J]. Journal of Tianjin Agricultural University, 2020, 27(1): 15-20.

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: http://xuebao.tjau.edu.cn/EN/10.19640/j.cnki.jtau.2020.01.004

http://xuebao.tjau.edu.cn/EN/Y2020/V27/I1/15

References

[1] 陈玉琦. 玫瑰香葡萄在天津滨海地区的生产现状及发展趋势[J]. 河北林业科技,2006(4):36-37.
[2] 田淑芬. RAPD技术提高玫瑰香葡萄优系纯度研究展望[J]. 天津农业科学,2002,8(2):51-53.
[3] 齐向丽,师校欣,杜国强. ‘红国王’葡萄组织培养快速繁殖[J/OL]. 分子植物育种,http://kns.cnki.net/kcms/ detail/46.1068.S.20190327.1017. 004.html.
[4] 韩旭,武丽娟. 美国“红地球”葡萄无毒苗速繁技术研究[J]. 内蒙古林业科技,2012,38(3):25-28.
[5] 徐美隆,刘静,王巧珍,等. 酿酒葡萄“赤霞珠”组织培养快繁技术优化[J]. 北方园艺,2016(2):117-120.
[6] 蔡文博,段虹,王军,等. 4个鲜食葡萄品种组培快繁体系的建立[J]. 核农学报,2019,33(2):248-254.
[7] 柴慈江,严任玲,王震星,等. 葡萄试管苗土支撑培养带坨移栽研究[J]. 华北农学报,1995,10(1):116-119.
[8] 闵首军,李爱玲. 葡萄组织快繁技术研究[J]. 农学学报,2012,2(10):55-57.
[9] 冯发文,田群芳. 巨峰葡萄组织培养快繁技术研究[J]. 落叶果树,2011(2):6-8.
[10] 沈传进,王利民,张平,等. 鲜食葡萄组织培养快速繁育系统的建立[J]. 河北农业科学,2011,15(7):16-21.
[11] 戴彩虹. 山葡萄组织培养与离体嫁接亲和性研究[D]. 兰州:甘肃农业大学,2014.
[12] 柴慈江,魏志勇. 晚红葡萄试管苗快速繁殖技术研究[J]. 北方园艺,2005(1):61-62.
[13] 柴慈江,史燕山,骆建霞,等. 土壤做培养基支撑物对栒子试管苗生根培养的影响[J]. 天津农学院学报,2013,20(4):18-21.
[14] 柴慈江,卢兴霞,漆倩,等. 土壤做培养基支撑物对无核枣试管苗生根培养的影响[J]. 天津农学院学报,2017,24(3):28-31.
[15] 柴慈江,卢兴霞,苏卫国,等. 珠美海棠试管苗的土壤支撑生根培养和带坨移栽[J]. 植物生理通讯,2010,46(1):33-36.
[16] 柴慈江,赵桐琳,史燕山,等. 矮溲疏组培快繁中的茎芽增殖与生根培养研究[J]. 安徽农业科学,2010,38(17):8871-8873.

Tissue culture and rapid propagation of Vitis vinifera ‘Muscat Hamburg’ cultured in soil supporting medium

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 10

Recommended Articles

Metrics

Comments

[1]	Xue Yuan, Li Deping, Yi Yuanhui, Yang Dandan, Niu Weiyuan, Liu Yanjun. Optimization of tissue culture and rapid propagation system of dendrobium nobile [J]. Journal of Tianjin Agricultural University, 2024, 31(2): 12-17.
[2]	Li Deping, Chen Yiming, He Yuanqin, Dong Hongyu, Yi Yuanhui, Liu Yanjun. Study on influencing factors of tissue culture seedling growth of Dendrobium officinale [J]. Journal of Tianjin Agricultural University, 2024, 31(1): 18-23.
[3]	Yi Yuanhui, Liu Yanjun, Xu Dingfan, Ran Jing. Establishment of tissue culture rapid propagation system of Sorbaria sorbifolia [J]. Journal of Tianjin Agricultural University, 2023, 30(4): 8-11.
[4]	Ren Xiaoqin, YANG Jinghui, Liu Yanjun, Zhang Jingxin, Ma Xin, Feng Guohua. Study on tissue culture and rapid propagation of Paeonia ostii [J]. Journal of Tianjin Agricultural University, 2021, 28(4): 19-22.
[5]	LIAN Peng, DU Meng-qing, WANG Li-juan. Optimization of disinfection methods and hormone ratio in tissue culture of strawberry shoot tips [J]. Journal of Tianjin Agricultural University, 2019, 26(3): 21-25.
[6]	CHAI Ci-jiang, LU Xing-xia, QI Qian, SHA Lei. Effect of Soil Supporting Medium on Rooting of Stoneless Chinese Jujube Plantlet in Vitro [J]. Journal of Tianjin Agricultural University, 2017, 24(3): 28-31.
[7]	LIU Yan, CHEN Xiao-qiang, WANG Xiao-hong, DENG Lin-xia, ZHANG Ya-he, WANG Jing-tai, WANG Dong-yuan. Effects of Tissue Culture Conditions on Polysaccharide Contents of Dendrobium Draconis [J]. Journal of Tianjin Agricultural University, 2015, 22(3): 32-35.
[8]	YANG Jing-hui, LIU Yan-jun, LI Jian-ke, HUANG Jun-xuan, LI Shuang-yue, WU Chun-xia. Technology of Tissue Culture and Factory Production for Ornamental Tower Poplar [J]. Journal of Tianjin Agricultural University, 2012, 19(4): 9-11.
[9]	BI Qing, ZHENG Run-fang, CUI Jian, PENG Li-xin. Establishment of Tissue Culture System of Salt Tolerance Rosa rugosa var. of Transgenosis [J]. Journal of Tianjin Agricultural University, 2012, 19(4): 28-31.
[10]	WANG Ya-zhi, SUN Ning, ZHANG Lei, CHEN Xiao-qiang, XU Yan-ping. Tissue Culture of Ficus religiosa [J]. Journal of Tianjin Agricultural University, 2012, 19(2): 30-33.