HPLC法检测鸡蛋中DHA-EE和EPA-EE含量的条件优化

doi:10.19640/j.cnki.jtau.2019.04.013

Journal of Tianjin Agricultural University ›› 2019, Vol. 26 ›› Issue (4): 61-66.doi: 10.19640/j.cnki.jtau.2019.04.013

• Researches and Scientific Notes • Previous Articles Next Articles

Optimization of determination condition of DHA-EE and EPA-EE in eggs by high performance liquid chromatography（HPLC）

PAN Li, YU Xiao-xue^{Corresponding Author}, MENG Yu-fang, CHENG Xue-fang, LI Bing-jie, LU Si-pei, DAI Xin-tong, LI Liu-an, JIN Tian-ming

Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China

Received:2019-05-07 Published:2020-01-19

Abstract

Abstract: To detect the content of ethyl eicosapentaenoate（EPA-EE）and ethyl docosahexaenoate（DHA-EE）in derived egg samples by high performance liquid chromatography（HPLC）, the super C₁₈ chromatographic column was used to investigate the effects of proportion of organic phase, flow rate and column temperature on separation of EPA-EE and DHA-EE. The optimal chromatographic conditions were determined: isocratic elution, mobile phase of methanol and water（95:5, V/V）, flow rate of 1.0 mL/min, column temperature 30℃, detection wave length of 220 nm. Through this method, the concentration and peak area of EPA-EE and DHA-EE showed a good linear relationship, and the correlation coefficient R² were 0.999 2 and 0.999 0 respectively. And then the separate peaks of EPA-EE and DHA-EE in derived egg samples were detected. This method is rapid, simple and reproducible, and it provides ideas for the rapid detection of polyunsaturated fatty acids in eggs.

Key words: high performance liquid chromatography（HPLC）, eggs, ethyl eicosapentaenoate（EPA-EE）, ethyl docosahexaenoate（DHA-EE）

CLC Number:

S879.3

PAN Li, YU Xiao-xue, MENG Yu-fang, CHENG Xue-fang, LI Bing-jie, LU Si-pei, DAI Xin-tong, LI Liu-an, JIN Tian-ming. Optimization of determination condition of DHA-EE and EPA-EE in eggs by high performance liquid chromatography（HPLC）[J]. Journal of Tianjin Agricultural University, 2019, 26(4): 61-66.

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Optimization of determination condition of DHA-EE and EPA-EE in eggs by high performance liquid chromatography（HPLC）

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