珍珠梅组培快繁体系的建立

doi:10.19640/j.cnki.jtau.2023.04.002

Journal of Tianjin Agricultural University ›› 2023, Vol. 30 ›› Issue (4): 8-11.doi: 10.19640/j.cnki.jtau.2023.04.002

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Establishment of tissue culture rapid propagation system of Sorbaria sorbifolia

Yi Yuanhui, Liu Yanjun, Xu Dingfan, Ran Jing

College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300392, China

Received:2022-09-01 Online:2023-08-31 Published:2023-11-01

Abstract

Abstract: In order to establish a rapid propagation system for tissue culture of Sorbaria sorbifolia,in this study the stem segments of Sorbaria sorbifolia were used as explants to study the effects of different disinfection times, different basic media, hormone types and concentrations on in vitro culture of Sorbaria sorbifolia. The results showed that using a 2% effective chlorine sodium hypochlorite solution for disinfection for 8 minutes and inoculating the stem segment disinfectant had the most ideal effect; using WPM+GA3 5 mg/L as the primary culture medium, the germination rate of axillary buds was the highest, with an average growth length of 6.01 cm, and the growth effect was the most ideal; using MS+BA 0.5 mg/L+IAA 1.0 mg/L as the proliferation medium, the proliferation coefficient reached 8.13 after 30 days of cultivation, indicating the best effect; the optimal rooting medium is 1/2 MS+NAA 0.5 mg/L; using peat∶vermiculite∶perlite=1∶1∶1（v/v）as the transplant substrate, the survival rate of transplantation can reach over 90% after 30 days, while ensuring the integrity of the root system.

Key words: Sorbaria sorbifolia, rapid propagation, stem segment, axillary bud

CLC Number:

S685

Yi Yuanhui, Liu Yanjun, Xu Dingfan, Ran Jing. Establishment of tissue culture rapid propagation system of Sorbaria sorbifolia[J]. Journal of Tianjin Agricultural University, 2023, 30(4): 8-11.

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Establishment of tissue culture rapid propagation system of Sorbaria sorbifolia

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