Abstract: In order to explore the antioxidant, hypoglycemic and hypolipidemic effects of active substances in quinoa seedlings, ultrasonic-assisted ethanol extraction method was used to extract polyphenols from quinoa seedlings, and the DPPH free radical scavenging rate, α-amylase and α-glucosidase activities inhibition rate were determined, and the hypolipidemic activity was evaluated by in vitro simulated cholate binding experiment. The results showed that quinoa seedling extracts contained 2.58 mg/g polyphenols, and the DPPH free radical scavenging rate ranged from 35.44% to 65.19% in the determination range. The half maximal inhibitory concentrations（IC₅₀）of quinoa seedling extract on DPPH free radicals, α-amylase activity, and α-glucosidase activity were 0.555 mg/mL, 9.599 μg/mL and 13.272 μg/mL, respectively, and the inhibitory effect increased with increasing concentration. The binding rate of quinoa seedling extracts to sodium glycocholate and sodium taurocholate increased with the increase of concentration, and the maximum binding rates were 94.22% and 75.54%, respectively. The concentration of quinoa seedling polyphenols had a very significant positive correlation with DPPH free radical scavenging rate, α-amylase inhibition rate, α-glucosidase inhibition rate, sodium glycocholate binding rate and sodium taurocholate binding rate, indicating that quinoa seedling polyphenols have strong hypoglycemic and hypolipidemic activity in vitro.

Key words: quinoa seedlings, polyphenols, antioxidation, ultrasonic assisted extraction, hypoglycemic, hypolipidemic