lncRNA-HZ5对牛骨骼肌卫星细胞成肌分化的调控作用研究

Journal of Tianjin Agricultural University ›› 2017, Vol. 24 ›› Issue (3): 64-68.

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Effects of lncRNA-HZ5 on Myogenic Differentiation Process of Bovine Skeletal Muscle Satellite Cells

DING Xiang-bin, ZHANG Wei-ran, ZHANG Jun-xing, WANG Yi-min, LIU Xin-feng, GUO Hong

College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China

Online:2017-09-30 Published:2019-10-15

Abstract

Abstract: In order to investigate the effects of lncRNA in the myogenic differentiation process of bovine skeletal muscle satellite cells, a highly and differentially expressed lncRNA（lncRNA-HZ5）detected by RNA-seq technique was selected for further research. The expression changes of lncRNA-HZ5 during bovine muscle satellite cells myogenic differentiation process were identified by qRT-PCR. The expression of lncRNA-HZ5 in muscle satellite cells were down regulated by siRNA, then the cells were transferred to differentiation medium, the myogenic process were evaluated by myotube formation and MHC expression levels. The results showed that the expression of lncRNA-HZ5 were significantly changed during bovine muscle satellite cells myogenic differentiation process. The myotube number and MHC expression levels of siRNA transferred group were significantly higher than that of control group. It was found that inhibition of lncRNA-HZ5 expression using siRNA can promoting the myogenic differentiation process of bovine skeletal muscle satellite cells.

Key words: lncRNA-HZ5, bovine, skeletal muscle satellite cells, myogenic differentiation

CLC Number:

S823

DING Xiang-bin, ZHANG Wei-ran, ZHANG Jun-xing, WANG Yi-min, LIU Xin-feng, GUO Hong. Effects of lncRNA-HZ5 on Myogenic Differentiation Process of Bovine Skeletal Muscle Satellite Cells[J]. Journal of Tianjin Agricultural University, 2017, 24(3): 64-68.

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Effects of lncRNA-HZ5 on Myogenic Differentiation Process of Bovine Skeletal Muscle Satellite Cells

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