金鱼腐皮病病原菌的分离鉴定及药敏试验

Journal of Tianjin Agricultural University ›› 2017, Vol. 24 ›› Issue (2): 53-57.

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Isolation, Identification and Antibiotics Susceptibility of the Pathogenic Bacteria Isolated from the Putrid Skin Diseased Carassius Auratus

KANG Xue-wei¹, HU Xiu-cai¹, SUN Jin-hui¹, SUN Jing-feng¹, LÜ Ai-jun^{1,Corresponding Author}, SONG Ya-jiao², YEONG Yiksung¹, SHI Hong-yue¹, GUO Li¹

1. Tianjin Key Lab of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300384, China;
2. College of Fisheries, Henan Normal University, Xinxiang 453007, Henan Province, China

Received:2016-09-14 Online:2017-06-30 Published:2019-10-15

Abstract

Abstract: A bacterial strain was isolated from the liver of the Putrid Skin Diseased Carassius auratus, named KL-1. The morphological observation, physiological and biochemical characteristics, the cloning and sequencing of 16S rDNA and phylogenetic tree analysis, animal infection test and the antibiotic susceptibility analysis were carried out in this study. Results showed that KL-1 strain was a rod-shaped, gram-negative bacillus, it can produce acid and gas fromglucose, oxidase, catalase and lysine decarboxylase showed positive reaction; Furthermore, the 16S rDNA sequences was amplified by PCR method, and subcloned into pMD18-T vector, a product of sequencing was 1 507 bp in length. It had a 99.66% similarity of 16S rDNA sequence with Aeromonas veronii type strain ATCC 35624（X74684）, and clustered into one group with A. veronii, so the KL-1 strain was identified as A. veronii. Artificial infection test showed that the median lethal（LD₅₀）to zebrafish was 1.4×10⁷ cfu/mL. The susceptibility testing with different antibiotics showed that it was sensitive to cefixime, cefoperazone, cefotaxime, cephalexin, clindamycin, nalidixic acid, enrofloxacin, difloxacin, norfloxacin, and florfenicol, chloramphenicol etc.

Key words: goldfish, Aeromonas veronii, pathogenicity, drug sensitivity test

CLC Number:

S943

KANG Xue-wei, HU Xiu-cai, SUN Jin-hui, SUN Jing-feng, LÜ Ai-jun, SONG Ya-jiao, YEONG Yiksung, SHI Hong-yue, GUO Li. Isolation, Identification and Antibiotics Susceptibility of the Pathogenic Bacteria Isolated from the Putrid Skin Diseased Carassius Auratus[J]. Journal of Tianjin Agricultural University, 2017, 24(2): 53-57.

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Isolation, Identification and Antibiotics Susceptibility of the Pathogenic Bacteria Isolated from the Putrid Skin Diseased Carassius Auratus

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