microRNA-143对小鼠失神经肌肉萎缩中肌纤维的影响

Journal of Tianjin Agricultural University ›› 2017, Vol. 24 ›› Issue (2): 39-43.

• Researches and Scientific Notes • Previous Articles Next Articles

Effect of MicroRNA-143 on Myofiber of Denevated Muscle Atrophy in Mouse

ZHANG Hui-na, ZHANG Wei-ran, WANG Yi-min, LIU Xin-feng, GUO Hong, DING Xiang-bin^{Corresponding Author}

College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China

Received:2017-03-28 Online:2017-06-30 Published:2019-10-15

Abstract

Abstract: To investigate the effects of microRNA-143（miR-143）on denervated muscle atrophy in mouse,an amyotrophia mouse model of sciatic nerve resection was established, 10 nmol miR-143 mimics or 10 nmol miR-143 inhibitors were injected to denevated gastrocnemius. Then, the expression of miR-143 was detected by qRT-PCR, the wet weight of gastrocnemius and the cross section diameter of the muscle fibers were compared between the atrophying skeletal muscle and control. The results showed that the diameter of myofiber injected with miR-143 mimics was significantly lower than that of control group while the diameter of myofiber injected with miR-143 inhibitors was significantly higher than that of control group. The results suggested that miR-143 played a role in repairing denervated muscle atrophy.

Key words: microRNA-143, mouse, denevation, muscle atrophy, muscle fiber, gastrocnemius

CLC Number:

ZHANG Hui-na, ZHANG Wei-ran, WANG Yi-min, LIU Xin-feng, GUO Hong, DING Xiang-bin. Effect of MicroRNA-143 on Myofiber of Denevated Muscle Atrophy in Mouse[J]. Journal of Tianjin Agricultural University, 2017, 24(2): 39-43.

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: http://xuebao.tjau.edu.cn/EN/

http://xuebao.tjau.edu.cn/EN/Y2017/V24/I2/39

References

[1] 徐建广,顾玉东. 缺血对失神经支配骨骼肌萎缩影响的研究[J]. 中华手外科杂志,1999,15(3):175-177.
[2] Zamore P D,Haley B.Ribo-gnome:the big world of small RNAs[J]. Science,2005,309(5740):1519-1524.
[3] Bartel D P.MicroRNAs:genomics,biogenesis,mechanism,and function[J]. Cell,2004,116(2):281-297.
[4] Meister G,Tuschl T.Mechanisms of gene silencing by double-stranded RNA[J]. Nature,2004,431(7006):343-349.
[5] Townley-Tilson W H,Callis T E,Wang D. MicroRNAs 1,133,and 206:Critical factors of skeletal and cardiac muscle development,function,and disease[J]. International Journal of Biochemistry & Cell Biology,2010,42(8):1252-1255.
[6] 谷造华,冯小宁,李永平,等. miRNA-1和miRNA-133在大鼠失神经腓肠肌中的变化规律研究[J]. 中华细胞与干细胞杂志:电子版,2013,3(1):17-20.
[7] Strickland I T,Richards L,Holmes F E,et al.Axotomy-induced miR-21 promotes axon growth in adult dorsal root ganglion neurons[J]. Plos One,2011,6(8):e23423.
[8] Dey B K,Pfeifer K,Dutta A.The H19 long noncoding RNA gives rise to microRNAs miR-675-3p and miR-675-5p to promote skeletal muscle differentiation and regeneration[J]. Genes & Development,2014,28(5):491-501.
[9] Cully M.Neuromuscular disorders:Beefing up the right splice variant to treat spinal muscular atrophy[J]. Nature Reviews Drug Discovery,2014,13(10):725.
[10] Troncoso R,Paredes F,Parra V,et al.Dexamethasone- induced autophagy mediates muscle atrophy through mitochondrial clearance[J]. Cell Cycle,2014,13(14):2281-2295.
[11] Hockerman G H,Dethrow N M,Hameed S,et al.The Ubr2 gene is expressed in skeletal muscle atrophying as a result of hind limb suspension,but not Merg1a expression alone[J]. European Journal of Translational Myology,2014,24(3):173-179.
[12] Johns N,Stephens N A,Fearon K C H. Muscle wasting in cancer[J]. International Journal of Biochemistry & Cell Biology,2013,45(10):2215-2229.
[13] Morimoto Y,Kondo Y,Kataoka H,et al.Heat treatment inhibits skeletal muscle atrophy of glucocorticoid- induced myopathy in rats[J]. Physiological Research,2015,64:897-905.
[14] Lecker S H,Goldberg A L,Mitch W E.Protein degradation by the ubiquitin-proteasome pathway in normal and disease states[J]. Journal of the American Society of Nephrology,2006,17(7):1807-1819.
[15] Banerjee A,Guttridge D C.Mechanisms for maintaining muscle[J]. Current Opinion in Supportive & Palliative Care,2012,6(4):451-456.
[16] 左乔,许家军. MicroRNA在神经系统不同部位特异分布及其在神经损伤再生中的变化和作用[J]. 生理科学进展,2011,42(4):261-268.
[17] Chen Y,Melton D W,Gelfond J A,et al.MiR-351 transiently increases during muscle regeneration and promotes progenitor cell proliferation and survival upon differentiation[J]. Physiological Genomics,2012,44(21):1042-1051.
[18] 王轶敏,张琬,张蔚然,等. microRNA-139对小鼠失神经肌肉萎缩中肌纤维的影响[J]. 天津农学院学报,2016,23(2):6-10.

Effect of MicroRNA-143 on Myofiber of Denevated Muscle Atrophy in Mouse

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 4

Recommended Articles

Metrics

Comments

[1]	WANG Yi-min, ZHANG Wan, ZHANG Wei-ran, DAI Yang, LIU Xin-feng, LI Xin, GUO Hong, DING Xiang-bin. Effects of MicroRNA-139 on Myofiber of Denevated Muscle Atrophy in Mouse [J]. Journal of Tianjin Agricultural University, 2016, 23(2): 6-10.
[2]	DUAN Xian-ping, QIN Shun-yi. Establishment of Kidney Pathological Model Injured by Gentamycin in Mice [J]. Journal of Tianjin Agricultural University, 2014, 21(4): 33-34.
[3]	DAI Yang, WANG Yi-min, LIU Xin-feng, FAN Han-lu, LI Ji-xia, GUO Hong, DING Xiang-bin. Isolated Culture and Identification of Mouse Skeletal Muscle Satellite Cells [J]. Journal of Tianjin Agricultural University, 2014, 21(1): 1-4.
[4]	ZHAO Cui-li, GAO Peng-yun, MA Ji-fei, BIAN Yan. Study on Acute Toxicity of Agaricus Blazei Polysaccharide Oral Liquid in Mice [J]. Journal of Tianjin Agricultural University, 2013, 20(2): 38-39.