猪流行性腹泻病毒YL112株的M基因序列分析及其在Vero细胞的传代适应性研究

Journal of Tianjin Agricultural University ›› 2017, Vol. 24 ›› Issue (2): 34-38.

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Sequence Analysis of the M gene of Porcine Epidemic Diarrhea VirusYL112 Strain and Its Adaptation to Vero Cells

LAN Ri-guo¹, ZHANG Shu-qing¹, ZHANG Jin-qiu², MIAO Jin-feng^{1,Corresponding Author}

1. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China;
2.National Research Center for Veterinary Vaccine Engineering and Technology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China

Received:2017-03-29 Online:2017-06-30 Published:2019-10-15

Abstract

Abstract: To analyze the genetic variation between PEDV YL112 strain and other isolates, the M gene of PEDV YL112 strain was amplified and sequenced. The M gene of PEDV YL112 strain shared 97.9%-99.8% homology with PEDV reference strains. Phylogenetic analysis indicated that PEDV YL112 strain was genetically close to isolates HLJBY and HN-XYYYP-2007 rather than the classic strain CV777. Furthermore, adaptation of PEDV YL112 to Vero cells was conducted and its titer significantly increased from 10^4.8TCID₅₀ /0.1mL to 10^7.6TCID₅₀ /0.1mL by cellular adaptation. These results may contribute to further study on the genetic evolution, viral virulence and new vaccines of PEDV.

Key words: PEDV YL112, M gene, sequence analysis, Vero cells, adaptation

CLC Number:

S858.28

LAN Ri-guo, ZHANG Shu-qing, ZHANG Jin-qiu, MIAO Jin-feng. Sequence Analysis of the M gene of Porcine Epidemic Diarrhea VirusYL112 Strain and Its Adaptation to Vero Cells[J]. Journal of Tianjin Agricultural University, 2017, 24(2): 34-38.

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Sequence Analysis of the M gene of Porcine Epidemic Diarrhea VirusYL112 Strain and Its Adaptation to Vero Cells

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