5-aza-dC联合TSA对不同供体细胞来源牛核移植胚胎体外发育的影响

Journal of Tianjin Agricultural University ›› 2012, Vol. 19 ›› Issue (2): 19-23.

• Researches and Scientific Notes • Previous Articles Next Articles

Effects of 5-aza-dC and TSA on Development of Embryos Cloned from Different Donor Cells

DING Xiang-bin, WANG Yi-min, GE Xiu-guo, LIU Xin-feng, LI Ji-xia, GUO Hong^{Corresponding Author}

Department of Animal Science, Tianjin Agricultural University, Tianjin 300384, China

Received:2012-05-03 Online:2012-06-20 Published:2019-11-12

Abstract

Abstract: ：To investigate the effects of 5-aza-dC and TSA on the development of embryos cloned from different donor cells, the fetal fibroblast cells, adult skin fibroblast cells, and cumulus cells were used as donor cells for nuclear transfer, the donor cells and cloned embryos were treated with TSA（50 nM, 12 h） and 5-aza-dC（20 nM, 72 h）, and the effects of these reagents on the development of cloned embryos were investigated. The results show that 5-aza-dC combined with TSA could significantly promoted the development potential of cloned embryos （P<0.01）, and the beneficial effects of TSA and 5-aza-dC on cloned embryos development were not of specific characteristics for the donor cells.

Key words: bovine, 5-aza-dC, TSA, nuclear transfer, donor cell

CLC Number:

S814.6

DING Xiang-bin, WANG Yi-min, GE Xiu-guo, LIU Xin-feng, LI Ji-xia, GUO Hong. Effects of 5-aza-dC and TSA on Development of Embryos Cloned from Different Donor Cells[J]. Journal of Tianjin Agricultural University, 2012, 19(2): 19-23.

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Effects of 5-aza-dC and TSA on Development of Embryos Cloned from Different Donor Cells

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