Abstract: By studying the effect of salt domestication of broccoli seeds on improving their salt tolerance, theoretical support is provided for salt tolerant cultivation of broccoli. Broccoli seeds were acclimated to salt for 7 days with 0, 10, 25, 50, 75, and 100 mmol/L NaCl. Using germination indicators as criteria, 75 and 100 mmol/L were selected as salt acclimation concentrations. Photosynthetic pigments, antioxidant enzyme activity, and osmoregulation substance content of their seedlings were measured under high salt treatment（225 mmol/L NaCl）. The results showed that salt acclimation of broccoli seeds significantly increased SOD activity, CAT activity, the content of photosynthetic pigments, proline, and soluble protein and significantly decreased the content of soluble sugar and MDA under high salt stress compared with CK2（salt control）. Compared with CK2, the 75 mmol/L domestication group showed a significant increase of 283.95%, 289.22%, 277.13%, and 276.64% in total chlorophyll, chlorophyll a, chlorophyll b, and carotenoids, with a significant increase of 16.64% in SOD activity, 120.76% in proline content, and 414.75% in POD activity. Comprehensive analysis shows that salt domestication of broccoli seeds can improve their salt tolerance, and membership function analysis results show that 75 mmol/L NaCl domestication of broccoli seeds has the best domestication effect.

Key words: broccoli, salt domestication, salt resistance, membership function, seed germination