天津市区公园土壤样品中弓形虫污染情况的调查

doi:10.19640/j.cnki.jtau.2018.04.011

Journal of Tianjin Agricultural University ›› 2018, Vol. 25 ›› Issue (4): 48-51.doi: 10.19640/j.cnki.jtau.2018.04.011

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Investigation of Toxoplasma gondii contamination in soil samples of parks in urban areas of Tianjin city

XU Huan, DONG Su-jie, ZHAO Zhen-nan, SU Miao, QIAN Li-min, SONG Qi-qi^{Corresponding Author}

College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China

Received:2018-05-14 Online:2018-12-20 Published:2019-11-12

Abstract

Abstract: In order to investigate the contamination status of Toxoplasma gondii in soil samples of parks in urban areas of Tianjin city, high copy DNA fragment B1 gene of Toxoplasma gondii was selected to be the diagnostic gene, and soil samples from thirty five parks and five universities were studied as research objects. Genomic DNA was extracted from the soil samples, and the contamination status of Toxoplasma gondii oocyst in soil samples from spring-summer months and autumn-winter months were detected by PCR method. The results showed that the positive rate of soil samples from spring-summer months was 11.11% （10/90）, while the positive rate of soil samples from autumn-winter months was 2.22%（2/90）. The contamination of Toxoplasma gondii was seasonal, and there is a downward trend from spring-summer months to autumn-winter months.

Key words: Toxoplasma gondii, urban areas of Tianjin city, parks, soil samples, contamination

CLC Number:

S855.9

XU Huan, DONG Su-jie, ZHAO Zhen-nan, SU Miao, QIAN Li-min, SONG Qi-qi. Investigation of Toxoplasma gondii contamination in soil samples of parks in urban areas of Tianjin city[J]. Journal of Tianjin Agricultural University, 2018, 25(4): 48-51.

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Investigation of Toxoplasma gondii contamination in soil samples of parks in urban areas of Tianjin city

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