嗜杀酵母的生物学特性及其应用

天津农学院学报 ›› 2017, Vol. 24 ›› Issue (1): 83-87.

嗜杀酵母的生物学特性及其应用

王麟¹, 谭春明^1,2, 于刚¹, 杨贤庆¹, 戚勃¹, 杨少玲^{1,通信作者}

1. 中国水产科学研究院南海水产研究所广州 510300;
2. 中国海洋大学食品科学与工程学院,山东青岛 266003

收稿日期:2016-05-06 出版日期:2017-03-31 发布日期:2019-10-15
通讯作者: 杨少玲（1979-）,女,广东广州人,助理研究员,硕士,主要从事水产品加工及高值化利用研究。E-mail：shaoling278@163.com。
作者简介:王麟（1980-）,男,山东莱州人,工程师,博士,主要从事海洋酵母多样性及生物活性物质研究。E-mail：linwang@mail.sdu.edu.cn。
基金资助:
广东省海洋渔业科技与产业发展专项“广谱抗菌活性的海洋嗜杀因子筛选及制备关键技术研究”（A201501C13）

Biological Properties of Killer Yeasts and Their Applications

WANG Lin¹, TAN Chun-ming^1,2, YU Gang¹, YANG Xian-qing¹, QI Bo¹, YANG Shao-ling^{1,Corresponding Author}

1. South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China;
2. College of Food Science and Engineering, Ocean University of China, Qingdao 266003, Shandong Province, China

Received:2016-05-06 Online:2017-03-31 Published:2019-10-15

摘要/Abstract

摘要： 嗜杀酵母在生长繁殖过程当中所产的嗜杀因子能够抑制或杀死某些特定的微生物,而这种毒素对于酵母本身却没有嗜杀作用。将嗜杀酵母应用于工业生产中,可以抑制野生酵母的污染、净化空气、提高产品质量,在发酵当中对体系环境的净化和生物稳定性具有较好的效果;一些海洋嗜杀酵母因能杀灭某些海洋动物的致病菌,而被应用于海水养殖业;嗜杀酵母分泌的嗜杀因子可作为抑制某些病原酵母及类酵母的抗真菌药制剂;此外,嗜杀酵母在基因工程和转基因植物当中也有应用,用作为研究酵母菌分子生物学的良好基因工程载体以及大量合成和分泌嗜杀因子以对抗病原真菌的侵染。本文就嗜杀酵母的种类、生物特性及嗜杀因子作用机理进行了综合论述,概述了嗜杀酵母在不同领域的应用,并对其应用前景进行了展望。

关键词: 嗜杀酵母, 嗜杀因子, 作用机理, 应用前景

Abstract: Killer yeast in the process of growth and reproduction of the killer toxin could inhibit or kill certain microorganisms, and the killer toxin of its yeast did not bloodthirsty. The killer yeast used in industrial production, can inhibit the wild yeast pollution and purify the air, improve the quality of products, in which fermentation purification and biological stability of system environment has good effect; Some marine killer yeast for pathogenic bacteria can kill certain marine animal, it is used in aquaculture; Killer yeast secretion of the killer factor can be used as antifungal drug preparations inhibiting certain pathogenic yeast and yeast; in addition, killer yeast in gene engineering and transgenic plants also have to use, as a study of yeast molecular biology good gene engineering vectors and large scale synthesis and secrete killer factor to fight the infection of pathogenic fungi. This paper discusses the killer yeast species, biological characteristics and toxicity mechanism, an overview of the killer yeast in different areas use and the application prospect.

Key words: killer yeast, killer toxin, mechanism of action, application prospect

中图分类号:

TS201.3

王麟, 谭春明, 于刚, 杨贤庆, 戚勃, 杨少玲. 嗜杀酵母的生物学特性及其应用[J]. 天津农学院学报, 2017, 24(1): 83-87.

WANG Lin, TAN Chun-ming, YU Gang, YANG Xian-qing, QI Bo, YANG Shao-ling. Biological Properties of Killer Yeasts and Their Applications[J]. Journal of Tianjin Agricultural University, 2017, 24(1): 83-87.

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: http://xuebao.tjau.edu.cn/CN/

http://xuebao.tjau.edu.cn/CN/Y2017/V24/I1/83

参考文献

[1] Bevan E A,Makower M.The physiological basis of the killer character in yeast[J]. Proc Int Congr Genet,1963,1:202-203.
[2] 王祥红,贾仁洁,李静,等. 嗜杀酵母的生物学功能及其应用[J]. 生物技术通报,2013(4):33-38.
[3] Schmitt M J,Breinig F.The viral killer system in yeast: from molecular biology to application[J]. FEMS Microbiol,2002,26(3):257-276.
[4] Chi Z M,Liu G L,Zhao S F,et al.Marine yeasts as biocontrol agents and producers of bio-products[J]. Appl Microbiol Biotechnol,2010,86(5):1227-1241.
[5] Magliani W,Conti S,Travassos L R,et al.Fromyeastkiller toxins to antibiobodies and beyond[J]. FEMS Microbiol Lett,2008,288(1):1-8.
[6] Liu G L,Chi Z,Wang G Y,et al.Yeast killer toxins,molecular mechanisms of their action and their applications[J]. Critical Reviews in biotechnology,2015,35(2):222-234.
[7] Wickner R B.Double-stranded and single-stranded RNA viruses of Saccharomyces cerevisiae[J].Annual Review in Microbiology,1992,46(1):347-375.
[8] Schmitt M J,Breinig F.Yeast viral killer toxins:lethality and self-protection[J]. Nat Rev Microbiol,2006,4(3):212-221.
[9] ColubevW I,Shabalin Y. Microcin production by the yeast Cryptococcus humicola[J]. FEMS Microbiological Letter,1994,119(1/2):105-110.
[10] Yamamoto T,Imai M,Tachihana K,et al.Application of monoclonal antibodies to the isolation and characterization of a killer toxin secreted byHansenula mrakii[J]. FEBS Lett,1986,195(1/2):253-257.
[11] Gunge N,Tamaru A,Ozawa F,et al.Isolation and characterization of linear deoxyribonucleic acid plasmids from Kluyveromyces lactis and the plasmid-associated killer character[J]. Journal of Bacteriology,1981,145(1):382-390.
[12] Middelbeek E J,Hermans J M.Production,purification and properties of a Pichia kluyveri killer toxin[J]. Antonie van Leeuwenhoek,1979,45(3):437-450.
[13] Philliskink G,Young T W.The occurrence of killer character in yeasts of various genera[J]. Antonie van Leeuwenhoek,1975,41(1):147-151.
[14] Kimura T,Kitamoto N,Ohla Y.Structural relationships among killer toxins secreted from the killer strains of the genus Williopsis[J]. Journal of Fermentation and Bioengineering,1995,80(1):85-87.
[15] Bussey H,Skipper N.Membrane mediated killing of Saccharomyces cerevisiae by glycoproteins from Torulopsis glabrata[J]. Journal of Bacteriology,1975,124(1):476-483.
[16] Radler F,Herzberger S,Schonig L,et al.Investigation of a killer strain of Zygosaccharomyces bailii[J]. Journal of General Microbiology,1993,193(3):495-500.
[17] Wang L,Yue L X,Chi Z M,et al.Marine killer yeasts active against a yeast strain pathogenic to crab Portunus trituberculatus[J]. Diseases of Aquatic Organisms,2008,80(3):211-218.
[18] Suzuki C,Yamada K,Okada N,et al.Isolation and characterization of halotolerant killer yeasts from fermented foods[J]. International Conference on Biomedical Engineering,2014,53(10):2593-2597.
[19] Hidalgo P,Flores M.Occurrence of the killer character in yeasts associated with Spanish wine production[J]. Food Micro-biol,1994,11(2):161-167.
[20] 王贵双,张柏林. 嗜杀苹果酒酵母的筛选与鉴定[J].食品与发酵工业,2005,31(12):35-39.
[21] Imamura T,Kawamoto M,Takaoka Y.Characteristics of main mash infected by killer yeast in sake brewing and the nature of its killer factor[J]. Journal of Fermentation Technology,1974,52:293-299.
[22] 赵静静,李艳. 嗜杀酵母的生物学特性及应用展望[J].酿酒科技,2010(9):68-71.
[23] 彭莹. 土星拟威尔酵母WC91-2菌株嗜杀因子和β-1,3-葡聚糖酶的研究[D]. 青岛:中国海洋大学,2010.
[24] Wickner R B.Double-stranded and single-stranded RNA viruses of Saccharomyces cerevisiae[J]. Annual Review in Microbiology,1992,46(1):347-375.
[25] Izgu F,Altinbay D,Acun T.Killer toxin of Pichia anomala NCYC 432;purification characterization and its exo- R-1,3-glucanase activity[J]. Enzyme & Microb Technol,2006,39(4):669-676.
[26] Magliani W,Stefania C,Mara G,et al.Yeast killer system[J]. Clinical Microbiology Reviews,1997,10:369-380.
[27] Izgu F,Altinbay D,Sertkaya A.Enzymic activity of the K5-type yeast killer toxin and its characterization[J]. Biosci Biotech & Biochem,2005,69(11):2200-2206.
[28] 王贵双. 嗜杀酵母的筛选及其在苹果酒发酵中的应用[D]. 北京:北京林业大学,2006.
[29] Klassen R,Paluszynski J P,Wemhoff S,et al.The primary targetof the killer toxin from Pichia acaciae is tRNA^Gln[J]. Mole Microbiol,2008,69(6):81-97.
[30] Vadasz A,Sfranken D B.Properties of a wine yeast an-tagonist,Saccharomyces cerevisiae T206[J]. South African Journal for Enology & Viticulture,2002,23:39-47.
[31] Klassen R,Meinhardt F.Linear plasmids pWR1A and pWR1B of the yeast Wingea robertsiae are associated with a killer phenotype[J]. Plasmid,2002,48(2):142-148.
[32] 詹金花,杨幼慧,谭余良,等. 嗜杀酵母的研究进展及其在酿酒工业中的应用[J]. 中国酿造,2007,26(6):1-4.
[33] Fleet G H,Heard G M.Yeast-growth during fermentation[M]. Philadelphia:Wine Microbiology and Biotechnology,Harwood Academic Pub,1993.
[34] Todd B E N,Fleet G H,Henschke P A. Promotion of autolysis through the interaction of killer and sensitive yeasts: potential application in sparkling wine production[J]. American Journal of Enology & Viticulture,2000,51(1):65-72.
[35] 鲍晓明,秦玉静,高东. 电融合构建嗜杀酿酒酵母及其发酵性能的研究[J]. 工业微生物,1995,25(4):25-31.
[36] 曾少敏,刘雁然,翁明辉. 芒果酒的研制[J]. 食品与机械,2000,5(79):22-23.
[37] Sulo P,Michlcakova S,Reiser V.Construction and properties of k1 type killer wine yeasts[J]. Biotech Lett,1992(14):55-60.
[38] 曾鹏. 应用嗜杀生香酵母提高清酒质量的研究[J]. 酿酒科技,1992(3):12-13.
[39] David E S,Ludovina G,Teresa G,et al.Yeast diversity in the Mediterranean strawberry tree(Arbutus unedo L.)fruits’fermentations[J]. Food Research International,2012,47(1):45-50.
[40] Antonio S,Eva N,Enrique B,et al.Ustilago maydis killer toxin as a new tool for the biocontrol of the wine spoilage yeast Brettanomyces bruxellensis[J]. International Journal of Food Microbiology,2011,145(1):147-154.
[41] Santos A,Navascue´s E,Bravo E,et al.Ustilago maydis killer toxin as a new tool for the biocontrol of the wine spoilage yeast Brettanomyces bruxellensis[J]. Intern J Food Microbiol,2011,145(1):47-54.
[42] Lowes K F,Shearman C A,Payne J,et al.Prevention of yeast spoilage in feed and food by the yeast mycocin HMK[J]. Appl Environ Microbiol,2000,66(3):1066-1076.
[43] Claudia P,Cristina R,Serena M,et al.Efficacy of killer yeasts in the biological control of Penicillium digitatum on Tarocco orange fruits(Citrus sinensis)[J]. Food Microbiology,2012,30(1):219-225.
[44] Santos A,Marquina D.Killer toxin of Pichia membranifaciens and its possible use as a biocontrol agent against grey mould disease of grapevine[J]. Microbiol,2004,150(8):2527-2534.
[45] Wang X H,Chi Z M,Yue L,et al.A marine killer yeast against the pathogenic yeast strain in crab(Portunus trituberculatus)and an optimization of the toxin production[J]. Microbiol Res,2007,162(1):77-85.
[46] 黄庭钰. 嗜杀酵母Killer yeast的杀伤性质和酵母的病毒假说[J]. 微生物学杂志,2000,5(1):67-69.
[47] Cassone A,De Bernardis F,Conti S,et al.Antilmdies,killer toxins and antifungal immunoprotection:a lesson from nature?[J]. Immunol Today,1997,18(4):164-169.
[48] Kazuyoshi K,Masahiko S,Takayuki S.Occurence of killer yeast in Japanese wineries and their characteristics[J]. J Ferment Technol,1984,21(62):161-176.
[49] Kishida M,Tokunaga M,Katayose Y,et al.Isolation and genetic characterization of pGKL killer-insensitive mutants from Saccharomyces cerevisiae[J]. Biosci Biotech & Biochemi,1996,60(5):798-800.