天津农学院学报 ›› 2017, Vol. 24 ›› Issue (1): 5-7.

• 研究与简报 • 上一篇    下一篇

浓硫酸与温水浸种对决明子萌发的影响研究

王景仪, 孙宁通信作者, 王妍, 杨文琪, 潘志达   

  1. 天津农学院 农学与资源环境学院,天津 300384
  • 收稿日期:2016-01-05 出版日期:2017-03-31 发布日期:2019-10-15
  • 通讯作者: 孙宁(1979-),女,河北承德人,高级实验师,硕士,主要从事生物技术方面的教学与研究工作。E-mail:sning79@sina.com。
  • 作者简介:王景仪(1994-),女,江苏徐州人,学士,主要从事植物组织培养方面的工作。E-mail:649562049@qq.com。
  • 基金资助:
    天津市科技支撑计划项目“非洲紫罗兰新品种的引进及培育技术的研究”(09ZCKFNC01600); 天津市级大学生创新创业训练计划项目“决明子离体培养及细胞悬浮系的建立”(201510061050); 天津农学院精品课程建设项目“植物细胞工程”(无编号)

Effects of Concentrated Sulfuric Acid and Warm Water Soaking on the Seeds Germination of Cassia obtusifolia

WANG Jing-yi, SUN NingCorresponding Author, WANG Yan, YANG Wen-qi, PAN Zhi-da   

  1. College of Agronomy and Resource Environment, Tianjin Agricultural University, Tianjin 300384, China
  • Received:2016-01-05 Online:2017-03-31 Published:2019-10-15

摘要: 决明子是具较高医疗养生价值的中草药之一,属于硬实种子,因发芽率低导致其产量也较低。本文利用温水和不同浓度的浓硫酸浸种处理对种皮进行软化,发芽后检测决明子的发芽势、发芽率、叶绿素含量、游离脯氨酸含量等指标。结果表明:决明子在80%浓硫酸中浸种20 min或60 ℃温水浸种2 h处理下,发芽率均达95%以上,并可明显的促进幼苗的初期生长。

关键词: 决明子, 种皮软化, 发芽率, 幼苗

Abstract: Cassia obtusifolia is one of the Chinese herbal medicines with high value of medical health. The seed of C. obtusifolia is belonging to hard seeds, which results in the lower germination rate while the low yield. Warm water and different concentrated H2SO4 were used to soften seed-coat; and the indexes, such as its seeds’ germination potential, germination rate, chlorophyll content, free proline content, etc. were measured after the seeds germinating. The results showed that the seed germination rate was up to 95% under the soaking treatment of 80% H2SO4 with 20 min or 60 ℃ water with 2 h, and it could obviously promote the initial growth of seedlings.

Key words: Cassia obtusifolia L., seed -coat softening, germination rate, seedlings

中图分类号: