解淀粉芽孢杆菌LJ02抑菌气体分析

天津农学院学报 ›› 2016, Vol. 23 ›› Issue (3): 23-26.

解淀粉芽孢杆菌LJ02抑菌气体分析

郝象瑢¹, 卢志军², 王婧¹, 李娟¹, 王远宏^{1,通信作者}

1. 天津农学院园艺园林学院,天津300384;
2. 北京市植物保护站,北京100029

出版日期:2016-09-20 发布日期:2019-10-14

Study on Volatile Organic Compounds Inhibition of Bacillus amyloliquefaciens LJ02

HAO Xiang-rong¹, LU Zhi-jun², WANG Jing¹, LI Juan¹, WANG Yuan-hong^{1,Corresponding Author}

1. College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300384, China;
2. Beijing Plant Protection Station, Beijing 100029, China

Online:2016-09-20 Published:2019-10-14

摘要/Abstract

摘要： 利用气相色谱-质谱联用（GC-MS）探讨解淀粉芽孢杆菌（Bacillus amyloliquefaciens）LJ02菌株在LB固体培养中产生挥发性气体的种类,并以5种病原真菌为指示菌,用平板对扣法和分格平板法检测LJ02菌株产生的挥发性气体的抑菌活性,确定对黑腐皮壳菌（Valsa mali）抑制作用的有效气体成分。结果表明：解淀粉芽孢杆菌LJ02产生的挥发性气体对多种果类致病菌,特别是对黑腐皮壳菌（V. mali）、草莓灰霉病菌（Botrytis cinerea）、枣浆胞病菌（Alternaria spp.）有显著抑制效果,抑菌率在50%以上。LJ02菌株在代谢过程中可产生多种挥发性气体,其中4-乙基苯酚、2-丙基环己酮2种气体对黑腐皮壳菌（V. mali）有强烈的抑制效果。

关键词: 解淀粉芽孢杆菌, 挥发性气体, 气质联用

Abstract: The paper used GC-MS to identify the kinds of volatile products in LB solid-state cultivation of B. amyloliquefaciens LJ02, and took 5 kinds of pathogenic fungi as indicator bacteria with two-sealed-base-plates method and two compartment plate method to detect the antibacterial activity of volatile gas produced by LJ02 strain and determined which active gas ingredients had inhibition with V. mali. The results showed that the volatile products in LB solid state cultivation of B. amyloliquefaciens LJ02 had significant inhibitory effect on most of the fruit-causing fungus, especially V. mali, B. cinerea and Alternaria spp., the inhibitory rate reaching above 50%. B. amyloliquefaciens LJ02 in metabolic process can produce a variety of volatile gases, including 4-ethylphenol, 2-propylcyclohexanone have strong inhibitory effect on V. mali.

Key words: Bacillus amyloliquefaciens, volatile organic compounds, GC-MS

中图分类号:

S432.1

郝象瑢, 卢志军, 王婧, 李娟, 王远宏. 解淀粉芽孢杆菌LJ02抑菌气体分析[J]. 天津农学院学报, 2016, 23(3): 23-26.

HAO Xiang-rong, LU Zhi-jun, WANG Jing, LI Juan, WANG Yuan-hong. Study on Volatile Organic Compounds Inhibition of Bacillus amyloliquefaciens LJ02[J]. Journal of Tianjin Agricultural University, 2016, 23(3): 23-26.

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链接本文: http://xuebao.tjau.edu.cn/CN/

http://xuebao.tjau.edu.cn/CN/Y2016/V23/I3/23

参考文献

[1] 罗萍. 梨储藏期病害生防菌及防腐保鲜的研究[D]. 雅安:四川农业大学,2009.
[2] 陈臻. 两株生防菌株对苹果树腐烂病菌拮抗作用的研究[D]. 兰州:甘肃农业大学,2013.
[3] 张鹏鹏. 几株植物根际促生细菌释放的挥发性物质对拟南芥及病原菌的影响[D]. 泰安:山东农业大学,2013.
[4] Fernando W G D,Ramarathnam R,Krishnamoorthy A S,et al. Identification and use of potential bacterial organic antifungal volatiles in biocontrol[J]. Soil Biology and Biochemistry,2005,37(5):955-964.
[5] Arrebola E,Jacobs R,Korsten L.Iturin A is the principal inhibitor in the biocontrol activity of Bacillus amyloliquefaciens,PPCB004 against postharvest fungal pathogens[J]. Journal of Applied Microbiology,2010,108(2):386-395.
[6] Osman M S,Sivakumar D,Korsten L.Effect of biocontrol agent Bacillus amyloliquefaciens and 1-methyl cyclopropene on the control of postharvest disease and maintenance of fruit quality[J]. Crop Protection,2011,30(2):173-178.
[7] Yuan J,Raza W,Shen Q,et al.Antifungal activity of Bacillus amyloliquefaciens NJN-6 volatile compounds against Fusarium oxysporum f. sp. cubense[J]. Applied and Environmental Microbiology,2012,78(16):5942-5944.
[8] 励建荣,朱丹实. 果蔬保鲜新技术研究进展[J]. 食品与生物技术学报,2012,31(4):337-347.
[9] 薛琼,刘跃军,向贤伟,等. 肉桂精油微囊化及其在果蔬保鲜中的应用[J]. 包装工程,2016(5):50-54.
[10] 闫训友,杜洪利,朱爱红,等. 丁香提取物对鲜切鸭梨保鲜效应的研究[J]. 食品工业科技,2016(4):347-350.
[11] Leelasuphakul W,Hemmanee P,Chuenchitt S.Growth inhibitory properties of Bacillus subtilis,strains and their metabolites against the green mold pathogen(Penicillium digitatum Sacc.)of citrus fruit[J]. Postharvest Biology & Technology,2008,48(1):113-121.
[12] 陈晓水,侯宏卫,边照阳,等. 气相色谱-串联质谱(GC-MS/MS)的应用研究进展[J]. 质谱学报,2013,34(5):308-320.
[13] Chaurasia B,Pandey A,Palni L M,et al.Diffusible and volatile compounds produced by an antagonistic Bacillus subtilis strain cause structural deformations in pathogenic fungi in-vitro[J]. Microbiological Research,2005,160(1):75-81.
[14] 陈华,郑之明,余增亮. 枯草芽孢杆菌JA脂肽类及挥发性物质抑菌效应的研究[J]. 微生物学通报,2008,35(1):1-4.
[15] 吴艳,闫豫君,赵思峰,等. 组合芽孢杆菌抑菌物质特性及其抑菌效果研究[J]. 西北农业学报,2007,16(5):266-270.
[16] Sisler E C,Serek M.Compounds controlling the ethylene receptor[J]. Botanical Bulletin-Academia Sinica Taipei,1999,40(1):1-7.
[17] Kittur F,Saroja N,Habibunnisa,et al. Polysaccharide- based composite coating formulation for shelf life extension of fresh banana and mango[J]. European Food Research & Technology,2001,213(4):306-311.
[18] 李家政. 果蔬自发气调包装原理与应用[J]. 包装工程,2011(15):33-38.
[19] Almenar E,Del V V,Catala R,et al.Active package for wild strawberry fruit(Fragaria vesca L.)[J]. Journal of Agricultural and Food Chemistry,2007,55(6):2240-2245.