关键词: 无花果, 微枝, 温室, 试管内生根, 移栽

Abstract: In order to study the method and effect of Ficus carica micro-shoots rooting in vitro in green-house, the cultural bottles containing the sterilized medium supported by soil in which the stem sections of Ficus carica plantlet in vitro were inoculated were respectively located in one shading net（about 39.0% of light transmission rate）or two shading net（about 16.0% of light transmission rate）in green-house for rooting, using the cultural bottles located in the constant temperature room as the control. Then the opening and closing of stoma was observed and the transplanting experiment was done for the Ficus carica plantlet rooted in vitro. The results showed that 100% of the stem sections cultured in green-house rooted and the root length, root number and leaf number of the plantlet cultured in green-house were significantly higher than those of the plantlet cultured in the constant temperature room. Without acclimated by opening bottle the stoma of the plantlet cultured in green-house had closing function in some extent. After transplanted the Ficus carica plantlet had the survival rate of 91.2% under the conditions without plastic film covering, without spraying and the lowest relative air humidity being about 60%. These results can obviously reduce the culture cost of the Ficus carica plantlet in vitro and promote the micro-propagation technology application of Ficus carica to the production.

Key words: Ficus carica, micro-shoot, green-house, in vitro rooting, transplanting