藏鸡源高抑菌活性乳酸菌筛选鉴定及其对鼠伤寒沙门菌感染小鼠的保护效应

doi:10.19640/j.cnki.jtau.2026.05.009

天津农学院学报 ›› 2026, Vol. 33 ›› Issue (5): 62-67.doi: 10.19640/j.cnki.jtau.2026.05.009

藏鸡源高抑菌活性乳酸菌筛选鉴定及其对鼠伤寒沙门菌感染小鼠的保护效应

孙宇材¹, 刘婷婷¹, 吉克娜薇¹, 蒋晓涛², 冯卫东³, 饶开晴^{1,通信作者}

1.西南民族大学动物医学四川省高等学校重点实验室,成都 610041;
2.四川省甘孜藏族自治州石渠县农牧农村局,四川甘孜藏族自治州 626000;
3.甘孜藏族自治州畜牧业科学研究所,四川甘孜藏族自治州 626000

收稿日期:2025-07-16 发布日期:2026-11-02
通讯作者: 饶开晴（1971—）,女,副教授,博士,研究方向为动物生长调控。E-mail：709661082@qq.com。
作者简介:孙宇材（2002—）,男,硕士在读,研究方向为动物生长调控。E-mail：2791725408@qq.com。共同第一作者：刘婷婷（1999—）,女,硕士在读,研究方向为动物生长调控。E-mail：2458027903@qq.com。
基金资助:
西南民族大学中央高校项目（ZYN2025096）

Screening and identification of lactic acid bacteria with high antimicrobial activity from Tibetan chicken and its protective effects against Salmonella Typhimurium infection in mice

SunYucai¹, Liu Tingting¹, Jike Nawei¹, Jiang Xiaotiao², Feng Weidong³, Rao Kaiqing^{1,Corresponding Author}

1. Key Laboratory of Animal Medicine at Southwest Minzu University of Sichuan Province, Chengdu 610041, China;
2. Department of Agriculture, Animal Husbandry and Rural Affairs, Shiqu County, Ganzi Tibetan Autonomous Prefecture, Ganzi Tibetan Autonomous Prefecture 626000, Sichuan Province, China;
3. Animal Husbandry Science Research Institute of Ganzi Tibetan Autonomous Prefecture, Ganzi Tibetan Autonomous Prefecture 626000, Sichuan Province, China

Received:2025-07-16 Published:2026-11-02

摘要/Abstract

摘要： 本试验旨在对藏鸡源产细菌素乳酸菌进行分离鉴定,筛选得到抑菌活性较高的产细菌素乳酸菌,并探究其在小鼠体内拮抗鼠伤寒沙门菌的能力及对小鼠腹腔巨噬细胞吞噬能力的影响。试验通过牛津杯法对从30份藏鸡粪中分离到的69株乳酸菌进行筛选,最终筛选出6株能同时抑制大肠杆菌和金黄色葡萄球菌的菌株,在排除有机酸、过氧化氢和蛋白酶K等的作用后仍具有较强的抑菌活性,经16S rRNA鉴定这6株乳酸菌为4株唾液乳杆菌、1株植物乳杆菌和1株卷曲乳杆菌。本试验选择对指示菌抑制作用最强的植物乳杆菌S1和卷曲乳杆菌S2作为后续试验的研究对象。植物乳杆菌S1和卷曲乳杆菌S2均产细菌素,且在胆盐和不同pH（2.0、3.0）环境中均可存活,对抗生素敏感。采用灌胃方式,将试验菌株S1、S2提前给予小鼠,再用鼠伤寒沙门菌感染小鼠。两菌株均能减少小鼠肝和脾中的鼠伤寒沙门菌数量,并提高小鼠腹腔巨噬细胞的吞噬率和吞噬指数。综上,从藏鸡粪便中分离筛选得到的植物乳杆菌S1和卷曲乳杆菌S2抑菌活性较高,对鼠伤寒沙门菌感染小鼠具有一定的保护作用。

关键词: 细菌素, 藏鸡, 植物乳杆菌, 卷曲乳杆菌, 体内抑菌保护作用

Abstract: This study aimed to isolate and characterize bacteriocin-producing lactic acid bacteria strains from Tibetan chicken samples, screen bacteriocin-producing lactic acid bacteria with high antibacterial activity, and explore their ability to antagonize Salmonella Typhimurium in mice and its effect on the phagocytosis of mouse peritoneal macrophages. In this experiment, 69 strains of lactic acid bacteria isolated from Tibetan chicken manure were screened by Oxford cup method, and 6 strains of lactic acid bacteria were obtained, which could inhibit both indicator Escherichia coli and Staphylococcus aureus, and still had strong antibacterial activity after excluding the effects of organic acids, hydrogen peroxide and protease K. The 6 strains were identified by 16S rRNA as 4 strains of Lactobacillus salivarius, 1 strain of Lactobacillus plantarum and 1 strain of Lactobacillus crispatus. In this experiment, Lactobacillus plantarum S1 and Lactobacillus crispatus S2, which had the strongest inhibition effect on indicator bacteria, were selected as the research objects of the subsequent experiment. Both Lacotabacillus plantarum S1 and Lactobacillus crispatus S2 produced bacteriocins, remained viable in the presence of bile salts and at different pH values（2.0, 3.0）, and were sensitive to antibiotics. The test strains S1 and S2 were administered to mice by oral gavage prior to infection with Salmonella Typhimurium. Both strains reduced the number of S. Typhimurium in the liver and spleen of mice and increased the phagocytic rate and phagocytic index of mouse peritoneal macrophages. In conclusion, L. plantarum S1 and L. crispatus S2 isolated from Tibetan chicken feces exhibited high antibacterial activity and conferred protective effects against Salmonella Typhimurium infection in mice.

Key words: bacteriocin, Tibetan chicken, Lactobacillus plantarum, Lactobacillus crispatus, in vivo antibacterial protective effect

中图分类号:

孙宇材, 刘婷婷, 吉克娜薇, 蒋晓涛, 冯卫东, 饶开晴. 藏鸡源高抑菌活性乳酸菌筛选鉴定及其对鼠伤寒沙门菌感染小鼠的保护效应[J]. 天津农学院学报, 2026, 33(5): 62-67.

SunYucai, Liu Tingting, Jike Nawei, Jiang Xiaotiao, Feng Weidong, Rao Kaiqing. Screening and identification of lactic acid bacteria with high antimicrobial activity from Tibetan chicken and its protective effects against Salmonella Typhimurium infection in mice[J]. Journal of Tianjin Agricultural University, 2026, 33(5): 62-67.

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链接本文: http://xuebao.tjau.edu.cn/CN/10.19640/j.cnki.jtau.2026.05.009

http://xuebao.tjau.edu.cn/CN/Y2026/V33/I5/62

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藏鸡源高抑菌活性乳酸菌筛选鉴定及其对鼠伤寒沙门菌感染小鼠的保护效应

Screening and identification of lactic acid bacteria with high antimicrobial activity from Tibetan chicken and its protective effects against Salmonella Typhimurium infection in mice

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