基于线粒体D-loop序列和COI基因的4个乌鳢群体遗传多样性分析

doi:10.19640/j.cnki.jtau.2026.05.008

天津农学院学报 ›› 2026, Vol. 33 ›› Issue (5): 54-61.doi: 10.19640/j.cnki.jtau.2026.05.008

基于线粒体D-loop序列和COI基因的4个乌鳢群体遗传多样性分析

李翰林, 郝爽, 何晓旭, 刘克明, 栾凯, 白晓慧^通信作者

天津市水产研究所,天津 300221

收稿日期:2024-11-25 发布日期:2026-11-02
通讯作者: 白晓慧（1982—）,女,高级工程师,博士,主要从事水产养殖与遗传育种方面的研究。E-mail：bxh123bxh@163.com。
作者简介:李翰林（1996—）,男,农艺师,硕士,主要从事水产动物病害与育种方面的研究。E-mail：1349341927@qq.com。
基金资助:
天津市科技计划项目（22ZYCGSN00820）; 天津市农业发展服务中心青年科技创新项目（zxkj202435,zxkj202433）

Genetic diversity and structure of four Channa argus populations based on mitochondrial D-loop and COI gene sequences

Li Hanlin, Hao Shuang, He Xiaoxu, Liu Keming, Luan Kai, Bai Xiaohui^{Corresponding Author}

Tianjin Fisheries Research Institute, Tianjin 300221, China

Received:2024-11-25 Published:2026-11-02

摘要/Abstract

摘要： 本研究通过线粒体D-loop和COI基因序列分析对天津及辽宁地区的4个乌鳢（Channa argus）群体的遗传多样性与遗传结构进行了探究。结果表明,D-loop和COI基因序列同源长度分别为818~823 bp、654~655 bp,A+T含量分别为61.0%、48.9%,C+G含量分别为39.0%、51.1%,D-loop序列存在明显的A/T碱基偏倚性;4个群体共94尾样本分别定义了32和23种单倍型。D-loop和COI基因序列的单倍型多样性（Hd）、核苷酸多样性（Pi）及平均核苷酸差异数（K）分别为0.883 1、0.005 7、4.628 0和0.833 9、0.042 6、27.746 0;群体间K2p遗传距离和遗传分化指数（Fst）分别为0.002 1~0.003 4和0.021 2~0.055 3。遗传分子方差分析（AMOVA）显示,4个群体遗传变异主要来源于群体内部。基于D-loop和COI基因,采用邻接法（Neighbor Joining,NJ）构建单倍型系统进化树表明,4个群体未能形成明显的地理分支。中性检验结果显示,乌鳢种群可能发生过种群扩张或自然选择。

关键词: 乌鳢（Channa argus）, 线粒体DNA控制区, 细胞色素氧化酶I, 遗传多样性

Abstract: In order to investigate the genetic diversity and differentiation of Channa argus, 94 samples were collected from Duliujian River（D）, Yuqiao Reservoir（K）, Jizhou（J）, Tianjin and Shenyang, Liaoning Province（L）. The genetic diversity and structure of four C. argus populations were analyzed by mitochondrial D-loop and COI gene sequences. The results showed that the homologous length of D-loop and COI gene sequences was 818-823 bp and 654-655 bp, and the content of A+T was 61.0% and 48.9%, and the content of C+G was 39.0% and 51.1%, respectively. The A/T base migration was obvious in D-loop sequence. A total of 94 samples from 4 populations defined 32 and 23 haplotypes, respectively. The haplotype diversity（Hd）, nucleotide diversity（Pi）and mean nucleotide difference（K）of D-loop and COI gene sequences were 0.883 1, 0.005 7, 4.628 0 and 0.833 9, 0.042 6, 27.746 0, respectively. The K2p genetic distance and the genetic differentiation index（Fst）were 0.002 1— 0.003 4 and 0.021 2—0.055 3, respectively. The analysis of genetic variation（AMOVA）showed that the genetic variation in four populations mainly came from within the population. The haplotype phylogenetic trees constructed by Neighbor Joining（NJ）method based on D-loop and COI genes showed that the four populations failed to form obvious geographical branches. The results of neutral test indicated that there may have been population expansion or natural selection. In conclusion, there was some genetic differentiation but no population differentiation among the four populations.

Key words: Channa argus, displacement loop region, cytochrome oxidase I, genetic diversity

中图分类号:

李翰林, 郝爽, 何晓旭, 刘克明, 栾凯, 白晓慧. 基于线粒体D-loop序列和COI基因的4个乌鳢群体遗传多样性分析[J]. 天津农学院学报, 2026, 33(5): 54-61.

Li Hanlin, Hao Shuang, He Xiaoxu, Liu Keming, Luan Kai, Bai Xiaohui. Genetic diversity and structure of four Channa argus populations based on mitochondrial D-loop and COI gene sequences[J]. Journal of Tianjin Agricultural University, 2026, 33(5): 54-61.

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http://xuebao.tjau.edu.cn/CN/Y2026/V33/I5/54

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基于线粒体D-loop序列和COI基因的4个乌鳢群体遗传多样性分析

Genetic diversity and structure of four Channa argus populations based on mitochondrial D-loop and COI gene sequences

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