Pseudoduganella armeniaca ZMN-3的全基因组测序与生物信息学分析

doi:10.19640/j.cnki.jtau.2026.03.011

天津农学院学报 ›› 2026, Vol. 33 ›› Issue (3): 58-64.doi: 10.19640/j.cnki.jtau.2026.03.011

Pseudoduganella armeniaca ZMN-3的全基因组测序与生物信息学分析

章阳^a, 李锦豪^a, 刘斌^a, 李烨平^a, 黄海东^a, 李晓雁^{b,通信作者}

天津农学院 a.农学与资源环境学院,b.食品科学与生物工程学院,天津 300392

收稿日期:2025-03-03 出版日期:2026-06-30 发布日期:2026-06-30
通讯作者: 李晓雁（1971—）,女,高级实验师,学士,研究方向为微生物多糖。E-mail：lxy@tjau.edu.cn。
作者简介:章阳（1998—）,女,硕士在读,研究方向为微生物多糖。E-mail：yangzhang2014@163.com。
基金资助:
天津市技术创新引导专项基金（23YDTPJC00610）

Whole genome sequencing and bioinformatics analysis of Pseudoduganella armeniaca ZMN-3

Zhang Yang^a, Li Jinhao^a, Liu Bin^a, Li Yeping^a, Huang Haidong^a, Li Xiaoyan^{b,Corresponding Author}

Tianjin Agricultural University, a. College of Agronomy and Resource Environment, b. College of Food Science and Bioengineering, Tianjin 300392, China

Received:2025-03-03 Online:2026-06-30 Published:2026-06-30

摘要/Abstract

摘要： 菌株ZMN-3是从荒漠化土壤中分离的一株产胞外多糖的Massilia属新菌。该属部分成员在GTDB数据库中的分类已被重新修订,但尚未从其他分类学角度分析。为阐明ZMN-3相关属成员关系,挖掘其潜在的生物学功能,本研究采用多基因进化树、基因注释功能及比较基因组学进行分析,并预测次级代谢产物基因簇。结果表明：菌株ZMN-3与Pseudoduganella plicata DSM 17505具有良好的共线性,结合MLSA和理化特征分析,支持菌株ZMN-3从Massilia属重新划分至Pseudoduganella属;该菌株基因组大小为6 337 491 bp,GC含量为66.5%,包含5 541个编码基因;COG、GO、KEGG数据库的注释结果显示,该菌株具有合成多种类型化合物修复土壤的潜力;经次级代谢产物基因簇预测,菌株ZMN-3具有产铁载体的功能。通过对菌株ZMN-3的生物信息学分析,挖掘其潜在生物学功能,可为菌株的后续开发利用提供数据支持与理论依据。

关键词: 假杜氏菌属, 全基因组测序, 基因功能注释, 比较基因组分析, 生物信息学分析

Abstract: Strain ZMN-3 is a new exopolysaccharide-producing strain of Massilia isolated from desertified soil. Recently, the classification of some members of this genus in GTDB has been revised, but the classification of these members has not been analyzed from other taxonomic perspectives. In order to clarify the membership relationship of ZMN-3 related genera and explore its potential biological functions, this study adopted polygene evolutionary tree analysis, gene annotation function analysis and comparative genomic analysis, and predicted the secondary metabolite gene cluster. The results showed that strains ZMN-3 and Pseudoduganella plicata DSM 17505 have good collinearity. The combination of these results with MLSA and physicochemical analysis supports the reclassification of strain ZMN-3 from the Massilia genus to the Pseudoduganella genus. The genome of strain ZMN-3 had a size of 6 337 491 bp, a GC content of 66.5%, and contained 5 541 coding genes. The annotation results of the COG, GO, and KEGG databases show that strain ZMN-3 has the potential to synthesize various types of compounds for soil remediation. The bioinformatics analysis of strain ZMN-3 to explore its potential biological functions can provide data support and theoretical basis for the subsequent development and utilization of strain ZMN-3.

Key words: Pseudoduganella, whole genome sequencing, gene function annotation, comparative genomic analysis, bioinformatics analysis

中图分类号:

Q939.11+2

章阳, 李锦豪, 刘斌, 李烨平, 黄海东, 李晓雁. Pseudoduganella armeniaca ZMN-3的全基因组测序与生物信息学分析[J]. 天津农学院学报, 2026, 33(3): 58-64.

Zhang Yang, Li Jinhao, Liu Bin, Li Yeping, Huang Haidong, Li Xiaoyan. Whole genome sequencing and bioinformatics analysis of Pseudoduganella armeniaca ZMN-3[J]. Journal of Tianjin Agricultural University, 2026, 33(3): 58-64.

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链接本文: http://xuebao.tjau.edu.cn/CN/10.19640/j.cnki.jtau.2026.03.011

http://xuebao.tjau.edu.cn/CN/Y2026/V33/I3/58

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Pseudoduganella armeniaca ZMN-3的全基因组测序与生物信息学分析

Whole genome sequencing and bioinformatics analysis of Pseudoduganella armeniaca ZMN-3

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