饲粮中添加酶制剂和抗菌肽对藏鸡肠道内源酶活性的影响

doi:10.19640/j.cnki.jtau.2024.05.007

天津农学院学报 ›› 2024, Vol. 31 ›› Issue (5): 46-51.doi: 10.19640/j.cnki.jtau.2024.05.007

饲粮中添加酶制剂和抗菌肽对藏鸡肠道内源酶活性的影响

王韩可¹, 冯卫东², 郑玉才¹, 李志雄¹, 倪建华³, 饶开晴^{1,通信作者}

1.西南民族大学动物医学四川省高等学校重点实验室,四川成都 610041;
2.四川省甘孜藏族自治州畜牧业科学研究所,四川甘孜 626000;
3.乡城县藏咯咯农业开发有限公司,四川甘孜 626700

收稿日期:2024-03-01 出版日期:2024-10-31 发布日期:2024-11-05
通讯作者: 饶开晴（1971—）,女,副教授,博士,研究方向为动物生长调控。E-mail：wkxwzz6@163.com。
作者简介:王韩可（1999—）,女,硕士在读,研究方向为动物生长调控。E-mail：2929607821@qq.com。
基金资助:
西南民族大学研究生创新型科研项目（ZD2022809）; 四川省科技计划项目（2021YFYZ0031,2023NSFSC0236）

Effects of dietary enzyme preparations and antimicrobial peptides on intestinal enzyme activities of Tibetan chickens

Wang Hanke¹, Feng Weidong², Zheng Yucai¹, Li Zhixiong¹, Ni Jianghua³, Rao Kaiqing^{1,Corresponding Author}

1. Sichuan Provincial Key Laboratory of Animal Medicine, Southwest Minzu University, Chengdu 610041, China;
2. Sichuan Ganzi Tibetan Autonomous Prefecture Animal Husbandry Research Institute, Ganzi 626000, Sichuan Province, China;
3. Xiangcheng County Zanggege Agricultural Development Co., Ltd., Ganzi 626700, Sichuan Province, China

Received:2024-03-01 Online:2024-10-31 Published:2024-11-05

摘要/Abstract

摘要： 试验旨在探究饲粮中添加非淀粉多糖（Non-starch polysaccharides,NSP）复合酶制剂、抗菌肽及酶肽复合剂对21~63日龄藏鸡小肠内源酶活性的影响。选取体重相近的14日龄藏鸡240只,随机分为4组,即基础饲粮组（对照组）、酶制剂组、抗菌肽组和酶肽复合组。预饲7天,42、63日龄采集藏鸡十二指肠、空肠、回肠肠道内容物,检测各组食糜中α-淀粉酶、脂肪酶、胰蛋白酶及糜蛋白酶活性。结果显示,与对照组相比,在42日龄时,酶肽复合添加显著提高了藏鸡小肠α-淀粉酶、糜蛋白酶活性及十二指肠和回肠脂肪酶活性（P<0.05）;酶制剂显著提高了藏鸡十二指肠、回肠α-淀粉酶活性和空肠、回肠脂肪酶活性（P<0.05）;在63日龄时,酶肽复合添加显著提高了藏鸡小肠糜蛋白酶活性,显著提高了十二指肠和回肠α-淀粉酶活性及十二指肠、空肠脂肪酶活性（P<0.05）;酶制剂显著提高了藏鸡小肠脂肪酶活性及十二指肠、回肠α-淀粉酶活性（P<0.05）;而抗菌肽对藏鸡小肠内源酶活性无显著影响（P>0.05）。结果表明,酶制剂单独添加及酶制剂和抗菌肽联合添加均能显著增强藏鸡小肠内源酶活性,且联合添加效果优于酶制剂单独添加。

关键词: 酶制剂, 抗菌肽, 藏鸡, 肠道内源酶

Abstract: The purpose of this experiment was to investigate the effects of dietary supplementation of non-starch polysaccharide （NSP）complex enzyme preparation, antimicrobial peptide and enzyme peptide complex on intestinal enzyme activities of Tibetan chickens aged 21 to 63 days. A total of 240 14-day-old Tibetan chickens with similar body weight were randomly divided into 4 groups: basal diet group（control group）, enzyme preparation group, antimicrobial peptide group and enzyme peptide complex group. Pre-feed for 7 days, the contents of duodenum, jejunum and ileum of Tibetan chickens at 42 and 63 days of age were collected, and the activities of α-amylase, lipase, trypsin and chymotrypsin in chymus of each group were detected. The results showed that, compared with the control group, the activities of α-amylase and chylin in small intestine, duodenal and ileal lipase in Tibetan chickens at 42 days of age were significantly increased in the enzyme-peptide complex group（P<0.05）; The enzyme preparation significantly enhanced the α-amylase activity in both the duodenum and ileum, the lipase activity of jejunum and ileum in Tibetan chickens（P<0.05）; At 63 days of age, the activities of chymotrypsin, α-amylase and duodenal and jejunal lipase in duodenum and ileum of Tibetan chickens were significantly increased with the supplementation of enzyme peptide complex（P<0.05）; The enzyme preparation significantly increased the activity of lipase in small intestine, α-amylase activity in duodenum and ileum of Tibetan chickens（P<0.05）; However, antimicrobial peptides had no significant effect on intestinal enzyme activity of Tibetan chickens（P>0.05）. The results showed that the enzyme preparation alone and the combination of enzyme preparation and antimicrobial peptide could significantly enhance the enzyme activity in small intestine of Tibetan chickens, and the combined addition effect was better than that of enzyme preparation alone.

Key words: enzyme preparation, antibacterial peptide, Tibetan chicken, intestinal enzyme

中图分类号:

S831.5

王韩可, 冯卫东, 郑玉才, 李志雄, 倪建华, 饶开晴. 饲粮中添加酶制剂和抗菌肽对藏鸡肠道内源酶活性的影响[J]. 天津农学院学报, 2024, 31(5): 46-51.

Wang Hanke, Feng Weidong, Zheng Yucai, Li Zhixiong, Ni Jianghua, Rao Kaiqing. Effects of dietary enzyme preparations and antimicrobial peptides on intestinal enzyme activities of Tibetan chickens[J]. Journal of Tianjin Agricultural University, 2024, 31(5): 46-51.

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: http://xuebao.tjau.edu.cn/CN/10.19640/j.cnki.jtau.2024.05.007

http://xuebao.tjau.edu.cn/CN/Y2024/V31/I5/46

参考文献

[1] 毕晓庆,程相雨,白玛央珍,等. 藏鸡健康养殖中存在的问题及产业化发展探讨[J]. 中国禽业导刊,2022,39(6):22-26.
[2] 强巴央宗,张浩,商鹏,等. 藏鸡屠宰性能及肉质特性[J]. 中国农业大学学报,2008,13(1):47-50.
[3] CHI C D,SHI M M,ZHAO Y T,et al.Dietary compounds slow starch enzymatic digestion:A review[J]. Frontiers in Nutrition,2022,9:1004966.
[4] CHOCT M,KOCHER A,WATERS D L E,et al. A comparison of three xylanases on the nutritive value of two wheats for broiler chickens[J]. British Journal of Nutrition,2004,92(1):53-61.
[5] 王冬群. 玉米型和小麦型饲粮中添加非淀粉多糖复合酶对肉鸡生产性能和消化道生理的影响[D]. 雅安:四川农业大学,2013.
[6] MENG X,SLOMINSKI B A,NYACHOTI C M,et al.Degradation of cell wall polysaccharides by combinations of carbohydrase enzymes and their effect on nutrient utilization and broiler chicken performance[J]. Poultry Science,2005,84(1):37-47.
[7] 张依量. 不同能量水平下非淀粉多糖酶在蛋鸡玉米—豆粕型日粮中的应用[D]. 保定:河北农业大学,2015.
[8] 李冠楠,夏雪娟,隆耀航,等. 抗菌肽的研究进展及其应用[J]. 动物营养学报,2014,26(1):17-25.
[9] CHOI S C,INGALE S L,KIM J S,et al.An antimicrobial peptide-A3:effects on growth performance,nutrient retention,intestinal and faecal microflora and intestinal morphology of broilers[J]. British Poultry Science,2013,54(6):738-746.
[10] 彭春芳,赵华,唐昱婷,等. 生物蛋白API对肉鸡生长性能和肠道健康的影响[J]. 四川农业大学学报,2022,40(5):676-682,689.
[11] MA J L,ZHAO L H,SUN D D,et al.Effects of dietary supplementation of recombinant plectasin on growth performance,intestinal health and innate immunity response in broilers[J]. Probiotics and Antimicrobial Proteins,2020,12(1):214-223.
[12] 岳道友,李亚明,赵杰. 微生态制剂与其他绿色饲料添加剂在家禽生产中的联合应用[J]. 中国家禽,2016,38(24):60-62.
[13] 郑腾,李昂,王光瑛. 家禽消化道酶分泌及活性影响因子的研究进展[J]. 畜牧与兽医,2005,37(6):59-62.
[14] CHOCT M.Feed non-starch polysaccharides for monogastric animals:classification and function[J]. Animal Production Science,2015,55(11):1360-1366.
[15] KUMAR V,SINHA A K,MAKKAR H P S,et al. Dietary roles of non-starch polysaccharides in human nutrition:a review[J]. Critical Reviews in Food Science and Nutrition,2012,52(10):899-935.
[16] MAHMOOD T,GUO Y.Dietary fiber and chicken microbiome interaction:Where will it lead to?[J]. Animal Nutrition,2020,6(1):1-8.
[17] JOZEFIAK D,RUTKOWSKI A,FRATCZAK M D.The effect of dietary fibre fractions from different cereals and microbial enzyme supplementation on performance,ileal viscosity and short-chain fatty acid concentrations in the caeca of broiler chickens[J]. Journal of Animal & Feed Sciences,2004,13:487-496.
[18] NGUYEN H T,BEDFORD M R,WU S B,et al.Soluble non-starch polysaccharide modulates broiler gastrointestinal tract environment[J]. Poultry Science,2021,100(8):101183.
[19] 陈德近,王乙茹,赵平,等. 复合非淀粉多糖酶制剂产品对肉鸡养殖生产效率及经济效益影响的研究[J]. 饲料博览,2021(7):5-9,18.
[20] LIU X,XING K,NING R,et al.Impact of combined α- galactosidase and xylanase enzymes on growth performance,nutrients digestibility,chyme viscosity,and enzymes activity of broilers fed corn-soybean diets[J]. Journal of Animal Science,2021,99(6):88.
[21] 奚刚. 日粮营养成分对动物基因表达的调控[J]. 动物营养学报,2000,12(1):2-7.
[22] 王恬,蒋正宇,周岩民,等. 饲料外源酶与动物内源消化酶的互作效应及机制研究[J]. 饲料与畜牧,2006(12):5-8.
[23] 叶晓梦,宋明强,孙晓晓,等. 饲粮粗蛋白质水平对肉鸡消化酶活性及能量、蛋白质代谢的影响[J]. 动物营养学报,2022,34(8):4988-4999.
[24] MANDAL S M,ROY A,GHOSH A K,et al.Challenges and future prospects of antibiotic therapy:from peptides to phages utilization[J]. Frontiers in Pharmacology,2014,5:105.
[25] 王新蕊. 饲粮添加蜜蜂抗菌肽Api-PR19对肉鸡生长性能、肠道健康和肠道菌群的影响[D]. 杨凌:西北农林科技大学,2023.
[26] 何丹林,温刘发,邓春柳,等. 蚕抗菌肽AD-酵母制剂对粤黄鸡肠道消化酶和饲料品质的影响[J]. 中国家禽,2004,26(7):12-13.
[27] 朱随亮,李玥,郑旭,等. 抗菌肽、酸化剂和功能性寡糖联合添加对肉仔鸡生长性能、肠道形态以及盲肠微生物区系的影响[J]. 动物营养学报,2021,33(8):4424-4437.
[28] 李莉娜,朱丽莉,粟朝芝,等. 蛋鸡复合饲料添加剂的筛选[J]. 中国畜牧兽医,2013,40(6):108-112.

饲粮中添加酶制剂和抗菌肽对藏鸡肠道内源酶活性的影响

Effects of dietary enzyme preparations and antimicrobial peptides on intestinal enzyme activities of Tibetan chickens

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 3

编辑推荐

Metrics

本文评价

[1]	张欣, 潘晨浩, 赵瑞利, 姜轩, 赵洋, 马吉飞, 于恩远, 李留安, 张振洲. 抗菌肽Temprine-La（FS）抑制耐甲氧西林伪中间型葡萄球菌生物被膜形成的研究[J]. 天津农学院学报, 2021, 28(1): 24-33.
[2]	王妍, 樊振中, 高金伟, 王晓梅. 动物抗菌肽诱导产生的方法及抗菌肽的应用前景[J]. 天津农学院学报, 2014, 21(1): 46-50.
[3]	胡烨, 马吉飞, 赵瑞利. 抗菌肽作为饲料添加剂的研究进展[J]. 天津农学院学报, 2013, 20(4): 48-51.