巴西龟弗氏柠檬酸杆菌病的临床症状及组织病理观察

doi:10.19640/j.cnki.jtau.2020.02.010

天津农学院学报 ›› 2020, Vol. 27 ›› Issue (2): 44-47.doi: 10.19640/j.cnki.jtau.2020.02.010

巴西龟弗氏柠檬酸杆菌病的临床症状及组织病理观察

罗福丽¹, 牛宇宸², 胡秀彩¹, 吕爱军^{1,通信作者}, 叶明皓¹, 孙敬锋¹

1.天津农学院水产学院天津市水产生态及养殖重点实验室,天津 300384;
2.天津大学化工学院,天津 300072

收稿日期:2019-05-23 出版日期:2020-06-30 发布日期:2020-07-03
通讯作者: 吕爱军（1973-）,男,教授,博士,主要从事水产动物微生物与免疫学研究。E-mail：lajand@126.com。
作者简介:罗福丽（1995-）,女,硕士在读,主要从事水产动物微生物与免疫学研究。E-mail：1838684865@qq.com。
基金资助:
国家大学生创新训练计划项目（201810061036）; 天津市自然科学基金项目（18JCYBJC29900）; 天津市水产产业技术体系创新团队（ITTFRS2017009）; 天津农学院综投平台项目（J01009030638）; 天津市水产生态及养殖重点实验室开放基金（TJAE201803）

Clinical signs and histochemical observation of Citrobacter freundii disease in turtle（Trachemys scripta elegans）

LUO Fu-li¹, NIU Yu-chen², HU Xiu-cai¹, LÜ Ai-jun^{1,Corresponding Author}, YE Ming-hao¹, SUN Jing-feng¹

1. Tianjin Key Laboratory of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300384, China;
2. School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China

Received:2019-05-23 Online:2020-06-30 Published:2020-07-03

摘要/Abstract

摘要： 为观察巴西龟弗氏柠檬酸杆菌病的临床症状及其组织病理学特征,采用人工感染试验、石蜡切片和HE染色方法进行组织病理研究。结果表明,感染发病龟主要表现为眼结膜发炎、口鼻皮肤溃烂,解剖可见肺部发黑,肠道淡黄色,无内容物、水样黏液增多等临床症状;组织病理学观察显示眼睑上皮组织色素细胞增多,胃和肠上皮细胞变性、坏死、脱落,心脏心肌纤维间隙变宽、坏死且有大量炎性细胞浸润,肺脏肺泡细胞与血管内皮细胞界限不清、肺泡壁毛细血管淤血,肝脏肝细胞坏死、中央静脉淤血、肝细胞索紊乱,肾脏肾小管上皮细胞坏死,肾小球出血,肌肉肌纤维间隙有大量炎性细胞浸润。以上结果为阐明龟类弗氏柠檬酸杆菌病的致病机理提供一定参考。

关键词: 巴西龟, 弗氏柠檬酸杆菌病, 组织病理

Abstract: In order to observe the clinical signs and its histopathological characteristics of Citrobacter freundii disease in turtle Trachemys scripta elegans, Histopathology was studied by artificial infection test, paraffin section and HE staining. The results showed that the main clinical signs of the infected sick turtle were conjunctivitis of the eye, ulceration of the oral and nasal skin; anatomy showed that the lungs were black, the intestinal tract was yellowish, with no content, and the water-like mucus increased. Histopathological observation showed the increase of pigment cells in eyelid epithelium, degeneration, necrosis and exfoliation of gastric and intestinal epithelial cells. The myocardial fibrils were widened, necrotic, and infiltrated with numerous inflammatory cells in the heart tissue. The boundary between pulmonary alveolar cells and vascular endothelial cells was unclear, and the alveolar wall had capillary congestion with Liver cell necrosis, central venous congestion, hepatic cord disorder, renal tubular epithelial cell necrosis, and glomerular hemorrhage. There was a large amount of inflammatory cell infiltration in muscle fiber space. The above results can provide some reference for elucidating the pathogenic mechanism of Citrobacter freundii disease of turtle.

Key words: Trachemys scripta elegans, Citrobacter freundii disease, tissue pathology

中图分类号:

S947.9

罗福丽, 牛宇宸, 胡秀彩, 吕爱军, 叶明皓, 孙敬锋. 巴西龟弗氏柠檬酸杆菌病的临床症状及组织病理观察[J]. 天津农学院学报, 2020, 27(2): 44-47.

LUO Fu-li, NIU Yu-chen, HU Xiu-cai, LÜ Ai-jun, YE Ming-hao, SUN Jing-feng. Clinical signs and histochemical observation of Citrobacter freundii disease in turtle（Trachemys scripta elegans）[J]. Journal of Tianjin Agricultural University, 2020, 27(2): 44-47.

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巴西龟弗氏柠檬酸杆菌病的临床症状及组织病理观察

Clinical signs and histochemical observation of Citrobacter freundii disease in turtle（Trachemys scripta elegans）

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