天津农学院学报 ›› 2019, Vol. 26 ›› Issue (1): 1-5.doi: 10.19640/j.cnki.jtau.2019.01.001

• 研究与简报 •    下一篇

玉米素灭菌方式和浓度对蓝莓试管苗增殖的影响

柴慈江1, 王坤英1, 张文文1, 刘春鹏2, 魏维1, 邹航1   

  1. 1. 天津农学院 园艺园林学院,天津 300384;
    2. 天津市泽芃农业科技有限公司蓟州区泽芃庄园蓝莓种植基地,天津 301900
  • 收稿日期:2018-09-17 出版日期:2019-03-20 发布日期:2019-10-09
  • 作者简介:柴慈江(1960-),男,教授,硕士,主要从事园艺植物组织培养方面的研究。E-mail:cijiang666@163.com。
  • 基金资助:
    国家星火计划项目(2008GA610015); 天津市星火计划项目(08ZHXHNC07000); 天津市林果现代农业产业技术体系创新团队项目(ITTFPRS2018002)

Effect of sterilizing method and concentration of ZT on blueberry plantlet in Vitro proliferation

CHAI Ci-jiang1, WANG Kun-ying1, ZHANG Wen-wen1, LIU Chun-peng2, WEI Wei1, ZOU Hang1   

  1. 1. College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300384, China;
    2.“ZEPENGManor” Blueberry Plantation, “ZEPENG”Agricultural Science and Technique Limited Company, Jizhou District, Tianjin 301900, China;
  • Received:2018-09-17 Online:2019-03-20 Published:2019-10-09

摘要: 在蓝莓试管苗增殖培养基配制过程中对ZT分别采取高温和过滤两种方式灭菌,以便探讨不同灭菌方式对ZT作用的影响;在WPM培养基中分别添加不同浓度的ZT,接种蓝莓试管苗茎段进行增殖培养。结果表明,在ZT浓度为1.0 mg/L和2.0 mg/L时,ZT高温灭菌和过滤灭菌两种处理的蓝莓试管苗繁殖系数无显著差异,高温灭菌对ZT的作用无明显不利影响;对于‘蓝丰’‘北陆’和‘爱国者’3个蓝莓品种,在ZT浓度为0.5~3.0 mg/L的范围内,ZT浓度为0.5 mg/L处理的试管苗繁殖系数较高,这3个蓝莓品种试管苗增殖培养基以采用WPM+ZT 0.5 mg/L为宜。

关键词: 玉米素, 高温灭菌, 浓度, 蓝莓试管苗, 增殖

Abstract: Both methods of ZT sterilized by high temperature and ZT sterilized by filteringwere respectively done inmaking media for blueberry plantlet in vitro proliferation to research on the effect of the sterilizing method on ZT function. The stem sections of blueberry plantlet in vitro were respectively inoculated in WPM media containing different concentration of ZT for shoot proliferation. The results showed that with the ZT concentration of 1.0 mg/L or 2.0 mg/L there were no significant difference in coefficient of multiplication of blueberry plantlet in vitro between methods of ZT sterilized by high temperature and ZT sterilized by filtering, and sterilizing with high temperature had no obvious unfavorable effect on ZT function. For blueberry varieties(‘Bluecrop’‘Northland’and‘Patriot’) the treatment of 0.5 mg/L ZT obtained the higher coefficient of multiplication among ZT concentration of 0.5-3.0 mg/L, and then the suitable multiplication medium for the three blueberry varieties was WPM+ZT 0.5 mg/L.

Key words: ZT, sterilizing with high temperature, concentration, blueberry plantlet in vitro, multiplication

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