PEG修饰Fe3O4纳米粒子（MNPs）的制备及催化氧化VB1研究

doi:10.19640/j.cnki.jtau.2018.04.009

天津农学院学报 ›› 2018, Vol. 25 ›› Issue (4): 38-43.doi: 10.19640/j.cnki.jtau.2018.04.009

PEG修饰Fe₃O₄纳米粒子（MNPs）的制备及催化氧化VB₁研究

刘泓, 毛祥双, 刘欢, 舒玥, 黄凤颖, 杨琳燕^通信作者

天津农学院动物科学与动物医学学院,天津 300384

收稿日期:2018-06-06 出版日期:2018-12-20 发布日期:2019-11-12
通讯作者: 杨琳燕（1982-）,女,讲师,博士,主要从事纳米药物载体研究。E-mail：y_linyan@163.com。
作者简介:刘泓（1996-）,女,本科在读,主要从事纳米药物载体研究。E-mail：1460300805@qq.com。
基金资助:
天津校级大学生创新创业训练计划项目（201810061120）; 国家自然科学基金（31572492,31372482）; 天津市教委科研计划项目（2017KJ190）; 省部共建药用资源化学与药物分子工程国家重点实验室资助课题（CMEMR2016-B12）; 天津农学院杰出科研业绩奖励资助项目（无编号）

Studies on catalytic oxidation of VB₁by PEG modified Fe₃O₄ nanoparticles

LIU Hong, MAO Xiang-shuang, LIU Huan, SHU Yue, HUANG Feng-ying, YANG Lin-yan^{Corresponding Author}

College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China

Received:2018-06-06 Online:2018-12-20 Published:2019-11-12

摘要/Abstract

摘要： 维生素B₁（VB₁）对人体正常生理功能起着重要作用,缺乏VB₁会导致人体出现多种疾病。某些温和的氧化剂可将VB₁氧化为含有荧光的硫色素,硫色素在紫外光照射下发出荧光,其荧光强度与VB₁浓度成正比。本文首先制备Fe₃O₄纳米粒子（MNPs）,通过优化实验条件,制备Fe₃O₄@SiO₂、Fe₃O₄@SiO₂-NH₂纳米粒子,对Fe₃O₄@SiO₂-NH₂样品进行双端羧基PEG修饰,得到Fe₃O₄@SiO₂-NH₂-PEG-COOH样品,设置3种不同的试验条件,利用PEG修饰产物催化VB₁为有荧光的硫色素,再经荧光分光光度计检测。研究结果表明,PEG修饰的Fe₃O₄ MNPs在不同H₂O₂浓度、不同温度及不同纳米粒子浓度时均可催化VB₁反应,产生荧光硫色素,但效果均不明显,而Fe₃O₄对温度和纳米粒子浓度的反应较大。

关键词: Fe₃O₄, PEG修饰, VB₁, 催化氧化

Abstract: VB₁ plays an important role in the normal life of human body. Relevant literature suggest that VB₁ could be oxidized to sulfur containing fluorescent pigment by some mild oxidant. Sulfur fluorescent dyes could fluoresced under UV irradiation, and the fluorescence intensity is proportional to the concentration of VB₁. In this study, Fe₃O₄, Fe₃O₄@SiO₂ and Fe₃O₄@SiO₂-NH₂ MNPs were prepared. Fe₃O₄@SiO₂-NH₂-PEG-COOH MNPs could be prepared by Fe₃O₄@SiO₂-NH₂ MNPs via PEG modification. Under three different test conditions, VB₁ could be oxidized by Fe₃O₄@SiO₂-NH₂-PEG-COOH MNPs, and the obtained sulfur fluorescent dyes could be detected by fluorescence spectrophotometer. The results showed that VB₁ could be catalyzed by the PEG modified Fe₃O₄MNPs in different H₂O₂concentration, different temperatures and different concentration of nanoparticles and produce fluorescent sulfur pigment, but the effect is not obvious. As for Fe₃O₄, response to temperature and concentration of nanoparticles is big.

Key words: Fe₃O₄, PEG modification, VB₁, catalytic oxidation

中图分类号:

O656.22

刘泓, 毛祥双, 刘欢, 舒玥, 黄凤颖, 杨琳燕. PEG修饰Fe₃O₄纳米粒子（MNPs）的制备及催化氧化VB₁研究[J]. 天津农学院学报, 2018, 25(4): 38-43.

LIU Hong, MAO Xiang-shuang, LIU Huan, SHU Yue, HUANG Feng-ying, YANG Lin-yan. Studies on catalytic oxidation of VB₁by PEG modified Fe₃O₄ nanoparticles[J]. Journal of Tianjin Agricultural University, 2018, 25(4): 38-43.

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PEG修饰Fe₃O₄纳米粒子（MNPs）的制备及催化氧化VB₁研究

Studies on catalytic oxidation of VB₁by PEG modified Fe₃O₄ nanoparticles

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