PEG修饰Fe3O4纳米粒子（MNPs）的制备及载药研究

doi:10.19640/j.cnki.jtau.2020.03.010

天津农学院学报 ›› 2020, Vol. 27 ›› Issue (3): 43-48.doi: 10.19640/j.cnki.jtau.2020.03.010

PEG修饰Fe₃O₄纳米粒子（MNPs）的制备及载药研究

辛亮亮¹, 舒玥², 刘欢², 胡雅稳², 杨琳燕^{2,通信作者}

1.天津药明康德新药开发有限公司国内新药研发服务部,天津 300457;
2.天津农学院动物科学与动物医学学院天津市农业动物繁育与健康养殖重点实验室,天津 300384

收稿日期:2019-07-03 出版日期:2020-10-10 发布日期:2020-10-10
通讯作者: 杨琳燕（1982-）,女,讲师,博士,主要从事纳米药物载体研究。E-mail：y_linyan@163.com。
作者简介:辛亮亮（1982-）,男,副研究员,硕士,主要从事药物合成研究。E-mail：xll_xll@126.com。
基金资助:
天津农学院杰出科研业绩奖励资助项目（无编号）; 国家自然科学基金（31572492,31372482）; 省部共建药用资源化学与药物分子工程国家重点实验室资助课题（CMEMR2016-B12）

Preparation and drug loading of PEG modified Fe₃O₄ nanoparticles

XIN Liang-liang¹, SHU Yue², LIU Huan¹, HU Ya-wen², YANG Lin-yan^{2,Corresponding Author}

1. Domestic Discovery Service Unit, Wuxi App Tec Co., Ltd., Tianjin 300457, China;
2. Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China

Received:2019-07-03 Online:2020-10-10 Published:2020-10-10

摘要/Abstract

摘要： 盐酸阿霉素（DOX）是一种抗肿瘤抗生素,通过抑制癌细胞遗传物质核酸的合成发挥作用,其抗瘤谱较广,对于多种肿瘤细胞均有杀灭作用。本研究首先通过共沉淀合成法制备Fe₃O₄纳米粒子（MNPs）,再依法制备Fe₃O₄@SiO₂以及Fe₃O₄@SiO₂–NH₂纳米粒子,然后对Fe₃O₄@SiO₂–NH₂样品进行单端羧基PEG和双端羧基PEG的修饰,得到Fe₃O₄@SiO₂–NH₂–PEG和Fe₃O₄@SiO₂–NH₂–PEG– COOH样品,利用PEG修饰产物装载不同质量的盐酸阿霉素（DOX）,得到Fe₃O₄@SiO₂–NH₂–PEG·DOX和Fe₃O₄@SiO₂–NH₂–PEG–COOH·DOX。荧光分光光度计检测结果表明,PEG修饰的Fe₃O₄纳米粒子（MNPs）载入不同质量的DOX除增加阿霉素的磁靶向性能外,因PEG的引入,生物相容性也得到增强。

关键词: Fe₃O₄, PEG修饰, DOX, 载药

Abstract: Doxorubicin hydrochloride（DOX）is an anti-tumor antibiotic that acts by inhibiting the synthesis of nucleic acid in cancer cells. DOX has a broad anti-tumor spectrum and has a killing effect on a variety of tumor cells. In this paper, Fe₃O₄ nanoparticles（MNPs）were prepared by co-precipitation synthesis method, while Fe₃O₄@SiO₂ and Fe₃O₄@SiO₂–NH₂ nanoparticles were prepared by optimizing experimental conditions. And then single and double carboxyl PEG were applied to modify Fe₃O₄@SiO₂–NH₂ samples. Fe₃O₄@SiO₂–NH₂–PEG and Fe₃O₄@SiO₂–NH₂–PEG–COOH samples could be obtained. PEG modified products were loaded with different quality of DOX. Fe₃O₄@SiO₂–NH₂–PEG·DOX and Fe₃O₄@SiO₂– NH₂–PEG–COOH·DOX samples could be obtained. After detection by a fluorescence spectrophotometer, a conclusion was drawn. The results showed that PEG–modified Fe₃O₄ nanoparticles（MNPs）loaded with different masses of DOX could increase the magnetic targeting properties of DOX, at the same time, biocompatibility could also be enhanced via the introduction of PEG.

Key words: Fe₃O₄, PEG modification, DOX, drug-loading

中图分类号:

O656.22

辛亮亮, 舒玥, 刘欢, 胡雅稳, 杨琳燕,. PEG修饰Fe₃O₄纳米粒子（MNPs）的制备及载药研究[J]. 天津农学院学报, 2020, 27(3): 43-48.

XIN Liang-liang, SHU Yue, LIU Huan, HU Ya-wen, YANG Lin-yan. Preparation and drug loading of PEG modified Fe₃O₄ nanoparticles[J]. Journal of Tianjin Agricultural University, 2020, 27(3): 43-48.

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http://xuebao.tjau.edu.cn/CN/Y2020/V27/I3/43

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PEG修饰Fe₃O₄纳米粒子（MNPs）的制备及载药研究

Preparation and drug loading of PEG modified Fe₃O₄ nanoparticles

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