DNA甲基化的植物学意义及其研究方法

天津农学院学报 ›› 2017, Vol. 24 ›› Issue (1): 77-82.

DNA甲基化的植物学意义及其研究方法

李坤怿¹, 袁文娅², 王鹏文^{1,通信作者}

1. 天津农学院农学与资源环境学院,天津 300384;
2. 天津市农作物研究所,天津 300384

收稿日期:2016-07-14 出版日期:2017-03-31 发布日期:2019-10-15
通讯作者: 王鹏文（1957-）,男,辽宁盖州人,教授,博士,主要从事作物遗传育种方面的研究。E-mail: pengwenw@sina.com。
作者简介:李坤怿（1992-）,男,新疆昌吉人,硕士在读,研究方向：作物遗传育种。E-mail: 845613986@qq.com。
基金资助:
天津市农科院院长基金项目“盐胁迫下玉米杂交种与亲本间DNA甲基化差异性分析”（15011）

Botany Meaning and Research Methods of DNA Methylation

LI Kun-yi¹, YUAN Wen-ya², WANG Peng-wen^{1,Corresponding Author}

1. College of Agronomy and Resource Environment, Tianjin Agricultural University, Tianjin 300384, China;
2. Tianjin Crops Research Institute, Tianjin 300384, China

Received:2016-07-14 Online:2017-03-31 Published:2019-10-15

摘要/Abstract

摘要： DNA中碱基的化学修饰一直是生命科学领域研究的热点之一。DNA甲基化作为DNA序列的修饰方式,是一种重要的表观遗传机制,能够在不改变DNA分子一级结构的情况下调节基因组的功能,在生命活动中起着重要的作用。对于受到环境胁迫的植物生命体基因表达调控过程中也有DNA甲基化变化的痕迹。本文对植物DNA甲基化的产生机制、功能,DNA甲基化在植物应对逆境胁迫中的作用以及用来分析检测DNA甲基化技术方法的原理特点进行综述,以便更好地理解植物DNA甲基化及其对环境胁迫的响应,为植物抗逆性研究及作物遗传改良提供理论参照。

关键词: 植物DNA甲基化, 基因表达调控, DNA甲基转移酶, 生物学功能

Abstract: In recent years, chemical modification in DNA bases has been one of the hottest areas of life science research. DNA methylation as one kind of modification way of DNA sequences, is an important epigenetic mechanism. It can regulate the expression of many vital genes while the base compositions of these genes don’t change, therefore DNA ethylation is very important to the plant’s grow progress. For the plants under environmental stress, the number of DNA methylation had changed during the regulation of gene expression. This paper introduces the mechanisms and biological functions of DNA methylation in plant, and its functions in plant response to environmental stress, and the principle and characteristics of the method for DNA methylation analysis. This would enable us to better understand the methylation of plant DNA and its response to environmental stress, and provide a theoretical reference for plant stress tolerance research and crop genetic improvement.

Key words: plant DNA methylation, gene expression and regulation, DNA methyltransferase, biological function

中图分类号:

李坤怿, 袁文娅, 王鹏文. DNA甲基化的植物学意义及其研究方法[J]. 天津农学院学报, 2017, 24(1): 77-82.

LI Kun-yi, YUAN Wen-ya, WANG Peng-wen. Botany Meaning and Research Methods of DNA Methylation[J]. Journal of Tianjin Agricultural University, 2017, 24(1): 77-82.

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