红鳍东方鲀鱼皮胶原蛋白的提取及主要理化性质的研究

天津农学院学报 ›› 2015, Vol. 22 ›› Issue (2): 6-10.

红鳍东方鲀鱼皮胶原蛋白的提取及主要理化性质的研究

万刚涛¹, 程民杰¹, 黄亚东², 王庆奎¹, 王冠¹, 邢克智^{1, *}

1. 天津农学院水产学院,天津 300384;
2. 天津海升水产养殖有限公司,天津 300272

收稿日期:2015-02-03 发布日期:2015-06-30
通讯作者: “十二五”国家科技支撑计划项目“黄渤海区鱼类工厂化健康养殖技术集成与示范”（2011BAD13B07）; 国家星火计划重大项目“水产品健康养殖、加工与质量安全控制产业化示范”（2013GA610002）; 天津市科技支撑计划重大项目“河鲀鱼良繁体系构建及大规模繁育”（12ZCDZNC05900）
作者简介:万刚涛（1989-）,男,山东青岛人,硕士在读,主要从事水产动物增养殖学方面的研究。E-mail：702080264@qq.com。
基金资助:
邢克智（1956-）,男,天津市人,教授,学士,主要从事水产养殖方面的研究。E-mail：kzxing6668@126.com。

Extraction and Physicochemical Characteristics of Collagen from Skin of Takifugurubripes

WAN Gang-tao¹, CHENG Min-jie¹, HUANG Ya-dong², WANG Qing-kui¹, WANG Guan¹, XING Ke-zhi^{1, *}

1. College of Fisheries, Tianjin Agricultural University, Tianjin 300384, China;
2. Tianjin Haisheng Aquaculture Co. Ltd, Tianjin 300272, China

Received:2015-02-03 Published:2015-06-30

摘要/Abstract

摘要： 以红鳍东方鲀鱼为原料,从其皮中提取酸溶性胶原蛋白（ASC）和酶溶性胶原蛋白（PSC）。在此基础上,分析了红鳍东方鲀胶原蛋白的结构组成和纯度、热变性、氨基酸组成等基本理化性质。结果表明：红鳍东方鲀鱼皮中酸溶性胶原蛋白和酶溶性胶原蛋白纯度分别高达83.19%和85.00%;将所得胶原蛋白分别经紫外光谱分析、傅里叶红外光谱分析和SDS-PAGE电泳测定,确定酸溶性和酶溶性胶原蛋白符合Ⅰ型胶原蛋白的特征,三螺旋结构完整;经测定,ASC和PSC热变性温度分别为29.85和30.10 ℃。

关键词: 红鳍东方鲀, 鱼皮, 胶原蛋白, 理化性质

Abstract: The acid-solubilized collagen（ASC）and pepsin-solubilized collagen （PSC）extracted from skin of Takifugurubripes were studied on its physicochemical properties, including the structure, purity, thermal denaturation temperature and the amino acid composition, etc. The results show that the ASC and PSC had high purity of 83.19 % and 85.00 %, respectively. The analysis of UV spectra, FTIR spectra and SDS-PAGE of ASC and PSC show that the two kinds of collagen were proved to be proteinⅠwith integrate triple-helix structure. And the thermal denaturation temperatures of ASC and PSC were 29.85 and 30.10 ℃, respectively.

Key words: Takifugurubripes, skin, collagen, physicochemical characteristics

中图分类号:

TS254.9

万刚涛, 程民杰, 黄亚东, 王庆奎, 王冠, 邢克智. 红鳍东方鲀鱼皮胶原蛋白的提取及主要理化性质的研究[J]. 天津农学院学报, 2015, 22(2): 6-10.

WAN Gang-tao, CHENG Min-jie, HUANG Ya-dong, WANG Qing-kui, WANG Guan, XING Ke-zhi. Extraction and Physicochemical Characteristics of Collagen from Skin of Takifugurubripes[J]. Journal of Tianjin Agricultural University, 2015, 22(2): 6-10.

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