天津农学院学报 ›› 2013, Vol. 20 ›› Issue (4): 18-20.

• 研究与简报 • 上一篇    下一篇

加拿大枫树离体再生体系的建立

刘艳军, 杨静慧通信作者, 李建科, 黄俊轩, 李双跃, 史滟滪   

  1. 天津农学院 园艺系,天津 300384
  • 收稿日期:2013-04-03 发布日期:2019-10-21
  • 通讯作者: 杨静慧(1961-),女,甘肃兰州人,教授,博士,主要从事园艺植物栽培、抗逆生理和分子育种研究。E-mail:jinghuiyang2@yahoo.com.cn。
  • 作者简介:刘艳军(1970-),男,天津市人,高级实验师,硕士,主要从事园艺植物组织培养和分子育种研究。E-mail:liuyanjun00a@126.com。
  • 基金资助:
    国家农业科技成果转化资金项目“耐盐耐旱林、草资源高效培育技术集成及重盐碱地、旱荒地改良示范”(2011GB2A100003); 天津市农业科技成果转化与推广项目“彩叶树种的引进、繁殖及农村环境的美化”(0703010)

Establishment of Regeneration System in Vitro of Canada Maple

LIU Yan-jun, YANG Jing-huiCorresponding Author, LI Jian-ke, HUANG Jun-xuan, LI Shuang-yue, SHI Yan-yu   

  1. Department of Horticulture, Tianjin Agricultural University, Tianjin 300384, China
  • Received:2013-04-03 Published:2019-10-21

摘要: 为了建立加拿大枫树的高效再生体系,以加拿大枫树无菌苗为试材,通过采用不同外植体和含有不同激素的培养基配方,获得了加拿大枫树的芽再生、根系诱导的最佳配方。结果显示:培养基配方2(MS+1.0 mg/L BA+0.2 mg/L IAA)的芽再生率较高(80%),平均每块外植体的再生芽数最高(2.7个芽/外植体块),再生芽的质量最好;下胚轴和叶柄的芽再生率为100%,子叶最低,为77.5%。下胚轴平均每块外植体的再生芽数最高(6.5个/块),叶柄的居中(3.6个/块),子叶的最低(2.6个/块);1/2 MS+0.2 mg/L NAA配方的枫树生根率为100%,单株生根数最高,为5.9条,且根系生长粗壮。

关键词: 芽分化, 外植体, 激素, 生根

Abstract: In order to establish the effective regeneration system of Canada maple, the aseptic seedlings of Canada maple were selected in the experiment, and the optimum formula for bud regeneration and rooting induction was obtained by using different explants and culture mediums containing different hormone. The results show that the bud regeneration rate was 80% on the No. 2 culture medium (MS+1.0 mg/L BA+0.2 mg/L IAA) with the highest average regeneration number per explant(2.7) and the best regeneration bud quality. The bud regeneration rate of petioles and hypocotyls was the highest (100%), while that of cotyledon was the lowest(77.5%) in explants. The average regeneration number of hypocotyls was the highest(6.5), while that of petioles was the second(3.6), and that of cotyledon was the lowest (2.6). The rooting rate of Canada maple seedlings was 100% on the medium 1/2 MS+0.2 mg/L NAA, the rooting number was the highest(5.9 per bud), and the roots were well developed.

Key words: bud regeneration, explant, hormone, rooting

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