土壤做培养基支撑物对栒子试管苗生根培养的影响

天津农学院学报 ›› 2013, Vol. 20 ›› Issue (2): 18-21.

土壤做培养基支撑物对栒子试管苗生根培养的影响

柴慈江, 史燕山, 骆建霞, 卢兴霞, 武旭娜

天津农学院园艺系,天津 300384

收稿日期:2013-03-04 发布日期:2019-10-18
作者简介:柴慈江（1960-）,男,天津市人,教授,硕士,主要研究方向为园艺植物组织培养。联系电话：（022）23781301。
基金资助:
国家科技部星火计划项目“优新木本观赏植物组织培养育苗规模化示范”（2008GA610015）; 天津市科委项目“优新木本观赏植物组织培养育苗规模化示范”（08ZHXHNC07000）

Effects of Soil Supporting Medium on Rooting Culture of Cotoneaster hjelmqvistii Plantlets in vitro

CHAI Ci-jiang, SHI Yan-shan, LUO Jian-xia, LU Xing-xia, WU Xu-na

Department of Horticulture, Tianjin Agricultural University, Tianjin 300384, China

Received:2013-03-04 Published:2019-10-18

摘要/Abstract

摘要： 以沙壤土和粘壤土分别做培养基支撑物进行栒子试管苗生根培养,试管苗的生根率、根长均明显高于琼脂支撑培养对照组。在沙壤土或粘壤土支撑培养条件下,培养基中不添加大量元素,可以显著提高栒子试管苗的生根率、根长等指标。以粘壤土做培养基支撑物,培养基中不添加微量元素和有机物,与添加者相比,生根率及根长无明显差异;培养基中添加蔗糖后,其生根率、根长等指标均明显高于未加蔗糖的处理,且培养基含蔗糖为1.5%的处理试管苗生根率达到93.3%,并显著高于含蔗糖为0.75%的处理。该研究结果可降低栒子试管苗成本,并为简化其移栽程序奠定基础。

关键词: 栒子, 试管苗, 土壤支撑, 培养基, 生根培养

Abstract: The stem sections of Cotoneaster hjelmqvistii plantlets in vitro were cultured in the medium supported by sandy loam soil and clay loam soil respectively for rooting, and the rooting rate and the root length of the plantlet cultured in the soil supporting medium were obviously higher than those of the plantlet cultured in the medium supported by agar. When cultured in the medium supported by the soil, the rooting rate and the root length of the plantlet in the medium without adding macro-elements were obviously higher than those in the medium with macro-elements added. In the medium supported by the clay loam soil, the rooting rate and the root length of the plantlet cultured in the medium without micro-elements and organic materials were obviously higher than those in the medium with micro-elements and organic materials added. Besides, in the medium supported by the clay loam soil, the rooting rate and the root length of the plantlet in the medium with sugar added were obviously higher than those of the plantlets cultured in the medium without sugar, and the rooting rate of the plantlet in the medium with 1.5% sugar was 93.3%, which were obviously higher than that of plantlet in the medium with 0.75% sugar. These results can reduce the cost and lay a foundation for simplifying the transplanting procedure of the Cotoneaster hjelmqvistii plantlets in vitro.

Key words: Cotoneaster hjelmqvistii, plantlet in vitro, soil supporting, culture medium, rooting culture

中图分类号:

柴慈江, 史燕山, 骆建霞, 卢兴霞, 武旭娜. 土壤做培养基支撑物对栒子试管苗生根培养的影响[J]. 天津农学院学报, 2013, 20(2): 18-21.

CHAI Ci-jiang, SHI Yan-shan, LUO Jian-xia, LU Xing-xia, WU Xu-na. Effects of Soil Supporting Medium on Rooting Culture of Cotoneaster hjelmqvistii Plantlets in vitro[J]. Journal of Tianjin Agricultural University, 2013, 20(2): 18-21.

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: http://xuebao.tjau.edu.cn/CN/

http://xuebao.tjau.edu.cn/CN/Y2013/V20/I2/18

[1]	薛缘, 李得萍, 易元慧, 杨丹丹, 牛崴湲, 刘艳军. 金钗石斛组培苗壮苗培养研究[J]. 天津农学院学报, 2024, 31(2): 12-17.
[2]	李得萍, 许丁帆, 何远秦, 杨森茹, 黄俊轩, 刘艳军. 锦带花组培快繁体系的建立[J]. 天津农学院学报, 2023, 30(3): 12-15.
[3]	黄洁萍, 柴慈江, 夏瑞, 董媛媛, 岑芬. 北美海棠试管苗生根培养研究[J]. 天津农学院学报, 2022, 29(1): 28-32.
[4]	任晓琴, 杨静慧, 刘艳军, 张景新, 马昕, 冯国华. 油用牡丹组培快繁研究[J]. 天津农学院学报, 2021, 28(4): 19-22.
[5]	朱霖, 柴慈江, 郭静, 时海东, 姚彤亮. 珠美海棠试管苗田间直接入地移栽技术研究[J]. 天津农学院学报, 2020, 27(2): 1-5.
[6]	王雪彤, 柴慈江, 郭静, 朱霖, 贺祥素. 土壤支撑培养下的‘玫瑰香’葡萄组培快繁[J]. 天津农学院学报, 2020, 27(1): 15-20.
[7]	郭静, 柴慈江, 史燕山, 骆建霞, 江文. 苹果砧木G.11试管苗增殖与生根培养研究[J]. 天津农学院学报, 2019, 26(4): 38-42.
[8]	贺祥素, 柴慈江, 郭静, 朱霖, 王雪彤, 冯涛. 樱桃李组织培养快速繁殖技术研究[J]. 天津农学院学报, 2019, 26(3): 1-6.
[9]	柴慈江, 王坤英, 张文文, 刘春鹏, 魏维, 邹航. 玉米素灭菌方式和浓度对蓝莓试管苗增殖的影响[J]. 天津农学院学报, 2019, 26(1): 1-5.
[10]	王然, 时海东, 柴慈江, 韦绕培, 宋秋月, 史燕山, 骆建霞, 张岩. 栒子试管苗露地环境中瓶内生根和直接入地移栽研究[J]. 天津农学院学报, 2018, 25(4): 1-5.
[11]	宋秋月, 韦绕培, 柴慈江, 王然, 时海东, 史燕山, 骆建霞. 露地与培养室环境对栒子微枝试管内生根与成活的影响[J]. 天津农学院学报, 2018, 25(2): 1-4.
[12]	柴慈江, 卢兴霞, 漆倩, 沙磊. 土壤做培养基支撑物对无核枣试管苗生根培养的影响[J]. 天津农学院学报, 2017, 24(3): 28-31.
[13]	柴慈江, 祁志彬, 汪强强, 卢兴霞, 史燕山, 骆建霞. 移栽时期和环境条件对栒子试管苗成活与生长的影响[J]. 天津农学院学报, 2017, 24(2): 22-24.
[14]	柴慈江, 曹海鹏, 王琼, 符玲巧, 骆建霞, 史燕山. 不同温室培养条件对栒子微枝试管内生根和成活的影响[J]. 天津农学院学报, 2015, 22(2): 11-15.
[15]	柴慈江, 汪强强, 骆建霞, 史燕山, 卢兴霞. 矮溲疏试管苗的土壤支撑生根培养和带坨移栽[J]. 天津农学院学报, 2014, 21(4): 22-25.