三种复方中草药对斑点叉尾鮰部分生化指标的影响

天津农学院学报 ›› 2012, Vol. 19 ›› Issue (3): 36-39.

三种复方中草药对斑点叉尾鮰部分生化指标的影响

郭永军¹, 乔之怡¹, 胡玉花², 陈成勋¹, 白东清¹, 丁莉莉¹, 刘志伟¹, 邢克智^{1,通讯作者}

1. 天津农学院水产科学系天津市水产生态及养殖重点实验室,天津 300384;
2. 天津市西青区中北镇农业服务中心,天津300112

收稿日期:2012-05-21 出版日期:2012-09-20 发布日期:2019-11-12
通讯作者: 邢克智（1956-）,男,天津市人,教授,学士,主要从事水产生态方面的研究。E-mail：kzxing@yahoo.com.cn。
作者简介:郭永军（1969-）,男,天津武清人,研究员,硕士,主要从事水产生态方面的研究。联系电话：（022）23788970。
基金资助:
天津市农业科技成果转化与推广项目“斑点叉尾鱼回人工繁殖及养殖技术示范”（0501130）

Effects of Three Compound Chinese Herbal Medicines on Some Biochemical Parameters in Ictalurus punctatus

GUO Yong-jun¹, QIAO Zhi-yi¹, HU Yu-hua², CHEN Cheng-xun¹, BAI Dong-qing¹, DING Li-li¹, LIU Zhi-wei¹, XING Ke-zhi^{1,Corresponding Author}

1. Tianjin Key Laboratory of Aqua-ecology and Aquaculture, Department of Fishery Science, Tianjin Agricultural University, Tianjin 300384, China;
2. Agricultural Service Center of Zhongbei Town, Xiqing District, Tianjin 300112, China

Received:2012-05-21 Online:2012-09-20 Published:2019-11-12

摘要/Abstract

摘要： 为研究复方中草药“鱼欢”、“肝泰乐”、“三黄粉”对斑点叉尾鮰生化指标的影响,将以上3种中草药按5 g/kg饵料的比例加到饵料中,连续喂食斑点叉尾鮰7 d后取样,测定血液和组织中的生化指标。结果表明：3种复方中草药均能提高血清和脾脏的SOD/MDA和CAT/MDA比值,提高血清和脾脏的抗氧化能力;3种复方中草药均能降低头肾的SOD/MDA和CAT/MDA比值,降低其抗氧化能力。“肝泰乐”和“鱼欢”能提高鳃的抗氧化能力,“三黄粉”和“鱼欢”能提高肌肉的抗氧化能力。3种复方中草药能降低肝胰脏中AST和ALT的活力。“肝泰乐”显著降低肝胰脏的LZM活力,“鱼欢”显著降低头肾的LZM活力,而“三黄粉”则显著提高肌肉的LZM活力。3种复方中草药均能提高血液的白细胞吞噬能力,降低肝胰脏的AST和ALT活力。

关键词: 中草药, 复方, 斑点叉尾鮰, 生化指标

Abstract: In order to test the effects of compound Chinese herbal medicine “Yuhuan”, “Gantaile” and “Sanhuangfen” on biochemical parameters in Ictalurus punctatus, an oral administration trial was performed. The medicines mentioned above were added to a commercial feed at 5 g/kg separately, and were administrated to the tested fish. The fish was sampled after a continuous 7-day feeding. The biochemical parameters in blood and tissues were assayed. The results show that all the medicines enhanced SOD/MDA and CAT/MDA ratio in serum and spleen. So the antioxidant capacity in serum and spleen was enhanced. All the medicines reduced the antioxidant capacity in head kidney by means of reducing SOD/MDA and CAT/MDA ratio. The “Gantaile” and “Yuhuan” enhanced the antioxidant capacity in gill, while “Sanhuangfen” and “Yuhuan” enhanced the antioxidant capacity in muscle. All the medicines reduced AST and ALT activities in hepato-pancreas. The LZM activity was significantly cut down in hepato-pancreas by “Gantaile” and in head kidney by “Yuhuan”, while “Sanhuangfen” boosted LZM activity in muscle significantly. All the medicines enhanced blood leucocytes phagocytosis and reduced AST and ALT activities in hepato-pancreas.

Key words: Chinese herbal medicine, compound prescription, Ictalurus punctatus, biochemical parameter

中图分类号:

S942

郭永军, 乔之怡, 胡玉花, 陈成勋, 白东清, 丁莉莉, 刘志伟, 邢克智. 三种复方中草药对斑点叉尾鮰部分生化指标的影响[J]. 天津农学院学报, 2012, 19(3): 36-39.

GUO Yong-jun, QIAO Zhi-yi, HU Yu-hua, CHEN Cheng-xun, BAI Dong-qing, DING Li-li, LIU Zhi-wei, XING Ke-zhi. Effects of Three Compound Chinese Herbal Medicines on Some Biochemical Parameters in Ictalurus punctatus[J]. Journal of Tianjin Agricultural University, 2012, 19(3): 36-39.

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参考文献

[1] 杨志刚,陈阿琴. 中草药饲料添加剂及其在水产养殖中的应用[J]. 水产科技情报,2008,35(2):76-79.
[2] 王鸿泰. 鱼类中草药药饵的配伍及制法[J]. 淡水渔业,1999,29(5):16-18.
[3] 简纪常,吴灶和. 中草药对建鲤非特异性免疫功能的影响[J]. 大连水产学院学报,2002,17(2):114-119.
[4] 谢丽玲,曹俊辉,杨素霞,等. 中草药作为饲料添加剂养殖罗非鱼试验[J]. 水产科学,2009,128(1):11-14.
[5] 张磊,于立新. 鲤鱼出血性疾病的防治[J]. 黑龙江水产,2008(5):17-19.
[6] 王丽岩. 渔用内服药在防治鱼病中的运用[J]. 黑龙江水产,2003(6):26,30.
[7] 王亚军. 大黄在水产动物疾病上的应用[J]. 渔业致富指南,2004(8):51-52.
[8] 姜峰. 混养鱼塘爆发性败血症的防治方法[J]. 科学养鱼,2006(9):59.
[9] 侯和菊. 中草药与鱼病防治[J]. 内陆水产,2006(10):28-29.
[10] 陈松. 草鱼出血病的治疗[J]. 科学养鱼,2009(11):59.
[11] 张云贵,刘祥云,李天俊. 生物化学实验指导[M]. 天津:天津科学技术出版社,2005:18-21.
[12] 李光友,王青. 中国对虾血细胞及其免疫研究[J]. 海洋与湖沼,1995,26(6):591-597.
[13] 蔡中华,邢克智,董双林. 维生素E对鲤鱼健康的影响[J]. 动物学报,2001,47(S1):120-124.
[14] 庞战军,周玫,陈瑗. 自由基医学研究方法[M]. 北京:人民卫生出版社,2000:20.
[15] 姚华,许宗利,姜平. 唾液溶菌酶测定结果数据处理的探讨[J]. 新疆医科大学学报,1999,22(1):68.
[16] 侯振江. 新编临床检验医学[M]. 北京:军事医学科学出版社,2004:7-8.
[17] Van der Oost R,Beyer J,Vermeulen N P E. Fish bioaccumulation and biomarkers in environmental risk assessment: a review[J]. Environ Toxicol Phar,2003,13(2):57-149.
[18] Viarengo A,Canesi L,Garcia M P,et al.Prooxidant processes and antioxidant defence systems in the tissues of the Antarctic scallop (Adamussiurn colbecki) compared with the Mediterranean scallop (Pecten jacobeus)[J]. Comp Biochem Physiolo,1995,111:119-126.
[19] 徐克成. 病毒性肝炎血清酶学试验及其临床意义[J]. 实用内科杂志,1991,11(9):454-456.