非洲紫罗兰花瓣细胞pH值测定及<em>F3′5′H</em>基因片段克隆

天津农学院学报 ›› 2012, Vol. 19 ›› Issue (1): 11-14.

• 研究与简报 • 上一篇下一篇

非洲紫罗兰花瓣细胞pH值测定及F3′5′H基因片段克隆

裴仁济^1,2, 陈小强¹, 孙宁¹, 张乃楠¹, 刘阳¹, 刘峰华², 张磊^{1,通讯作者}

1. 天津农学院农学系,天津 300384;
2. 天津天士力现代中药资源有限公司资源发展部,天津 300410

收稿日期:2011-08-13 出版日期:2012-03-20 发布日期:2019-11-12
通讯作者: 张磊（1952-）,男,天津市人,教授,学士,主要从事植物组织培养的教学与研究工作。E-mail：zl1952@yahoo.com.cn。
作者简介:裴仁济（1982-）,男,江苏泰州人,硕士,主要研究方向为植物细胞工程育种。E-mail：peirenji@sina.com。
基金资助:
天津市科技支撑计划重点项目“非洲紫罗兰新品种的引进和育种”（09ZCKFNC01600）; 天津市应用基础及前沿技术研究计划资助项目“兰花子房启动发育相关基因的克隆与表达”（10JCYBJC09000）

Petal Cell pH Value Determination and F3′5′H Gene Fragment Cloning of Saintpqulia ionantha

PEI Ren-ji^1,2, CHEN Xiao-qiang¹, SUN Ning¹, ZHANG Nai-nan¹, LIU Yang¹, LIU Feng-hua², ZHANG Lei^{1,Corresponding Author}

1. Department of Agronomy, Tianjin Agricultural University, Tianjin 300384, China;
2. Department of Resource Development, Tianshili Modern Chinese Medicine Resource Co. Limited, Tianjin 300410, China

Received:2011-08-13 Online:2012-03-20 Published:2019-11-12

摘要/Abstract

摘要： 以白色和蓝色品种的非洲紫罗兰舌状花瓣为材料,测定其细胞液pH值,并利用RT-PCR技术,以NCBI上登记的马铃薯、矮牵牛、龙胆草、金鱼草和瓜叶菊的F3′5′H基因片段保守区域为模板,克隆F3′5′H基因片段,为花色显色机理研究提供理论依据。实验结果表明：蓝色品种非洲紫罗兰细胞液的pH值大于白色品种非洲紫罗兰花瓣细胞液的pH值,随着花期的延长,两个品种非洲紫罗兰细胞液的pH值变化不断增大,且白色品种非洲紫罗兰花瓣细胞液的pH值变化大于蓝色品种非洲紫罗兰花瓣细胞液的pH值;其范围均在3.0~7.0之间,呈弱酸;在两种花色品种非洲紫罗兰中,都得到了F3′5′H基因片段的cDNA序列长度为392 bp,与马铃薯、矮牵牛、龙胆草、金鱼草和瓜叶菊的F3′5′H基因保守区域片段的同源性分别为63.0%、55.4%、52.8%、49.0%和47.7%。

关键词: 非洲紫罗兰, RT-PCR技术, F3′5′H基因, pH值, 花色

Abstract: The experiment used white and blue Saintpqulia ionantha cultivars′ linguoid petal as test materials to determine their pH values of cellular sap and clone F3′5′H gene fragment which was compared with the F3′5′H genes of Solanum tuberosum, Petunia hybrida, gentiana scabra bge, Snapdragon, Pericallis cruentia that were registered in NCBI by using the RT-PCR technique for the study of coloration mechanism. The results indicate that the pH value in blue Saintpqulia ionantha cultivar′ cellular sap was obviously higher than that in white Saintpqulia ionantha cultivar′ cellular sap, and the pH values of two Saintpqulia ionantha cultivars petal cell showed increasing trend, the changes of pH value were greater in white Saintpqulia ionantha cultivar′ cellular sap than those in blue Saintpqulia ionantha cultivar′ cellular sap. And the range of their pH values was between 3.0 and 7.0, which was faintly acid. In two Saintpqulia ionantha cultivars, the cDNA sequence of F3′5′H gene fragment were both 392 bp. Compared with the F3′5′H genes, conservative area of Solanum tuberosum, Petunia hybrida, gentiana scabra bge, Snapdragon, Pericallis cruentia, the homology of nucleotide sequence separately reached 63.0%, 55.4%, 52.8%, 49.0% and 47.7%.

Key words: Saintpaulia ionantha, RT-PCR technology, F3′5′H gene, pH value, flower color

中图分类号:

S681.2

裴仁济, 陈小强, 孙宁, 张乃楠, 刘阳, 刘峰华, 张磊. 非洲紫罗兰花瓣细胞pH值测定及F3′5′H基因片段克隆[J]. 天津农学院学报, 2012, 19(1): 11-14.

PEI Ren-ji, CHEN Xiao-qiang, SUN Ning, ZHANG Nai-nan, LIU Yang, LIU Feng-hua, ZHANG Lei. Petal Cell pH Value Determination and F3′5′H Gene Fragment Cloning of Saintpqulia ionantha[J]. Journal of Tianjin Agricultural University, 2012, 19(1): 11-14.

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: http://xuebao.tjau.edu.cn/CN/

http://xuebao.tjau.edu.cn/CN/Y2012/V19/I1/11

参考文献

相关文章 1

编辑推荐

Metrics

本文评价