小麦TaYODA 2D的克隆、表达分析及亚细胞定位

doi:10.19640/j.cnki.jtau.2026.05.001

天津农学院学报 ›› 2026, Vol. 33 ›› Issue (5): 1-8.doi: 10.19640/j.cnki.jtau.2026.05.001

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小麦TaYODA 2D的克隆、表达分析及亚细胞定位

常思宇, 温雨哲, 曹高燚, 陈小强, 谢晓东^通信作者，包曙光^通信作者

天津农学院农学与资源环境学院,天津 300392

收稿日期:2026-05-27 发布日期:2026-11-02
通讯作者: 谢晓东（1975—）,男,研究员,博士,主要从事分子育种方面的研究。E-mail：xiex@tjau.edu.cn。包曙光（1985—）,男,讲师,博士,主要从事分子育种方面的研究。E-mail：baoshuguang@tjau.edu.cn。
作者简介:常思宇（2004—）,女,本科在读,主要从事作物遗传育种研究。E-mail：sdzrx@163.com。
基金资助:
天津市大学生创新创业训练计划项目（202410061076）; 天津市科技计划项目（25ZXBTSN00030）; 河北省盐碱地综合利用关键技术研发与示范（YJZ2004001）

Cloning, expression analysis, and subcellular localization of TaYODA 2D gene from Triticum aestivum

Chang Siyu, Wen Yuzhe, Cao Gaoyi, Chen Xiaoqiang, Xie Xiaodong^{Corresponding Author}, Bao Shuguang^{Corresponding Author}

College of Agronomy and Resource Environment, Tianjin Agricultural University, Tianjin 300392, China

Received:2026-05-27 Published:2026-11-02

摘要/Abstract

摘要： YODA编码一种丝裂原活化蛋白激酶激酶激酶（MAPKKK）,在植物生长发育中发挥着重要的调控作用。为探究小麦中YODA的生物学功能,本研究从小麦叶片中克隆了小麦TaYODA 2D基因。该基因编码区全长2 670 bp,编码889个氨基酸,编码的蛋白为亲水的、不稳定的碱性蛋白。二级结构主要由无规卷曲、α-螺旋、延长链和β-折叠构成。此外,TaYODA 2D蛋白含有115个磷酸化位点和176个糖基化位点。启动子区域包含基本转录、激素响应、光响应、逆境诱导以及代谢与组织调控等顺式作用元件。系统进化分析显示,小麦TaYODA 2D蛋白与二穗短柄草亲缘关系较近。qRT-PCR结果显示,TaYODA 2D基因在根、胚芽鞘、茎、叶和小穗中均有表达;亚细胞定位分析表明,其编码蛋白可能定位于质膜和细胞质。本研究为解析小麦TaYODA 2D基因功能及育种应用提供了理论基础与基因资源。

关键词: 小麦, TaYODA 2D, 基因克隆, 表达分析, 亚细胞定位

Abstract: YODA encodes a mitogen-activated protein kinase kinase kinase (MAPKKK), which plays a crucial regulatory role in plant growth and development. To investigate the biological function of YODA in wheat, this study cloned the TaYODA 2D gene from wheat leaves. The gene coding region was 2 670 bp in length, encoding 889 amino acids, and produced a hydrophilic, unstable basic protein. The secondary structure was primarily composed of random coils, α-helices, extended chains, and β-sheets. In addition, the TaYODA 2D protein contained 115 phosphorylation sites and 176 glycosylation sites. The promoter region contained cis-acting elements essential for transcriptional regulation, hormone response, light response, stress induction, as well as metabolic and tissue regulation. Systematic evolutionary analysis revealed that the wheat TaYODA 2D protein shared a close phylogenetic relationship with Brachypodium distachyon. qRT-PCR results demonstrated that the TaYODA 2D gene was expressed in roots, coleoptile, stems, leaves, and spikelets. Subcellular localization analysis indicated that its encoded protein was likely localized to the plasma membrane and cytoplasm. This study provides a theoretical foundation and genetic resources for elucidating the function of the TaYODA 2D gene in wheat and its application in breeding.

Key words: wheat, TaYODA 2D, gene cloning, expression analysis, subcellular localization

中图分类号:

S512.1

常思宇, 温雨哲, 曹高燚, 陈小强, 谢晓东, 包曙光. 小麦TaYODA 2D的克隆、表达分析及亚细胞定位[J]. 天津农学院学报, 2026, 33(5): 1-8.

Chang Siyu, Wen Yuzhe, Cao Gaoyi, Chen Xiaoqiang, Xie Xiaodong, Bao Shuguang. Cloning, expression analysis, and subcellular localization of TaYODA 2D gene from Triticum aestivum[J]. Journal of Tianjin Agricultural University, 2026, 33(5): 1-8.

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小麦TaYODA 2D的克隆、表达分析及亚细胞定位

Cloning, expression analysis, and subcellular localization of TaYODA 2D gene from Triticum aestivum

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