转OsARP基因拟南芥对SA和6-BA处理的生理响应

doi:10.19640/j.cnki.jtau.2026.01.004

天津农学院学报 ›› 2026, Vol. 33 ›› Issue (1): 20-24.doi: 10.19640/j.cnki.jtau.2026.01.004

转OsARP基因拟南芥对SA和6-BA处理的生理响应

鹿祥瑞^a, 张耀方^b, 管政^a, 李志彬^a, 罗峰^a, 裴忠有^{a,通信作者}

天津农学院 a.农学与资源环境学院,b.基础科学学院,天津 300392

收稿日期:2024-09-10 出版日期:2026-02-28 发布日期:2026-02-05
通讯作者: 裴忠有（1967—）,男,教授,博士,主要从事农作物遗传育种及分子辅助育种方面的研究。E-mail：zhongyoupei@tjau.edu.cn。
作者简介:鹿祥瑞（1999—）,女,硕士在读,主要从事农作物遗传育种方面的研究。E-mail：17861622750@163.com。
基金资助:
国家重点研发计划“七大农作物育种”项目（2017YFD01005）; 天津市科技计划项目（21YFSNSN00140）; 天津市主要农作物智能育种重点实验室青年开放课题（KLIBMC2308）

Physiological responses of OsARP-transgenic Arabidopsis to SA and 6-BA treatments

Lu Xiangrui^a, Zhang Yaofang^b, Guan Zheng^a, Li Zhibin^a, Luo Feng^a, Pei Zhongyou^{a,Corresponding Author}

Tianjin Agricultural University, a. College of Agronomy and Resource Environment, b. College of Basic Science, Tianjin 300392, China

Received:2024-09-10 Online:2026-02-28 Published:2026-02-05

摘要/Abstract

摘要： 为探究水稻OsARP基因对不同激素处理下拟南芥生理特性的影响,以转过表达OsARP基因拟南芥幼苗为试验材料,分别用1、2 mmol/L SA溶液以及300、600 mg/L 6-BA溶液进行处理,测定拟南芥农艺性状和生理指标,并在含酸碱指示剂的MS培养基中观察其颜色变化。结果表明：经过不同浓度的SA和6-BA处理的转基因拟南芥在根长、株高、鲜质量、干质量、叶绿素含量、SOD活性、CAT活性、MDA及蔗糖含量上与野生型相比差异不显著,说明SA和6-BA未引起OsARP基因产生明显的生理响应,而转基因拟南芥的根部在含有两种不同指示剂的MS培养基中均发生了颜色改变,表明OsARP基因可能参与了细胞酸生长调控过程。

关键词: 拟南芥, OsARP基因, 外源激素, 生理学响应

Abstract: To investigate the effect of the rice OsARP gene on the physiological characteristics of Arabidopsis thaliana under different hormone treatments, OsARP-overexpressing Arabidopsis seedlings were used as experimental materials. The seedlings were treated with salicylic acid（SA）solutions at concentrations of 1 mmol/L and 2 mmol/L, as well as 6-benzylaminopurine （6-BA）solutions at 300 mg/L and 600 mg/L, respectively. The agronomic traits and physiological indices of Arabidopsis were determined, and the color change was observed in MS medium containing acid-base indicators. The results showed that compared with the wild type, the transgenic Arabidopsis treated with different concentrations of SA and 6-BA exhibited no significant differences in root length, plant height, fresh weight, dry weight, chlorophyll content, superoxide dismutase（SOD）activity, catalase（CAT）activity, malondialdehyde（MDA）content, or sucrose content. This indicated that SA and 6-BA treatments did not affect the physiological response mediated by the OsARP gene. However, the roots of transgenic Arabidopsis showed color changes in MS media containing two different indicators, suggesting that the OsARP gene may be involved in the regulation of cellular acid growth.

Key words: Arabidopsis thaliana, OsARP gene, exogenous hormones, physiological response

中图分类号:

Q945.78

鹿祥瑞, 张耀方, 管政, 李志彬, 罗峰, 裴忠有. 转OsARP基因拟南芥对SA和6-BA处理的生理响应[J]. 天津农学院学报, 2026, 33(1): 20-24.

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链接本文: http://xuebao.tjau.edu.cn/CN/10.19640/j.cnki.jtau.2026.01.004

http://xuebao.tjau.edu.cn/CN/Y2026/V33/I1/20

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转OsARP基因拟南芥对SA和6-BA处理的生理响应

Physiological responses of OsARP-transgenic Arabidopsis to SA and 6-BA treatments

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