性早熟中华绒螯蟹游离氨基酸的呈味分析及生态评价

doi:10.19640/j.cnki.jtau.2025.03.003

天津农学院学报 ›› 2025, Vol. 32 ›› Issue (3): 12-17.doi: 10.19640/j.cnki.jtau.2025.03.003

性早熟中华绒螯蟹游离氨基酸的呈味分析及生态评价

缪鈜烨^b, 刘雅楠^b, 高金伟^a,b, 贾旭颖^a,b, 周文礼^a,b, 张丹^{a,b,通信作者}

天津农学院a. 农业农村部智慧养殖重点实验室（省部共建）,b. 水产学院天津市水产生态及重点实验室,天津 300392

收稿日期:2025-04-13 出版日期:2025-06-30 发布日期:2025-07-02
通讯作者: 张丹（1988—）,女,讲师,博士,研究方向：水产养殖生态学。E-mail：zhangdan@tjau.edu.cn。
作者简介:缪鈜烨（2003—）,女,本科在读,主要从事水产养殖生态学方面研究。E-mail：misautumnmiao@163.com。
基金资助:
国家重点研发计划课题（2024YFD2401803）; 天津市教委科研计划项目（2023ZD001）; 天津市科技计划项目（24ZYCGSN00130）

Flavour analysis and ecological evaluation of free amino acids in sexually precocious Eriocheir sinensis

Miao Hongye^b, Liu Yanan^b, Gao Jinwei^a,b, Jia Xuying^a,b, Zhou Wenli^a,b, Zhang Dan^{a,b,Corresponding Author}

Tianjin Agricultural University, a. Key Laboratory of Smart Breeding（Co-construction by Ministry and Province）, b. Tianjin Key Lab of Aqua-ecology and Aquaculture, College of Fisheries, Tianjin 300392, China

Received:2025-04-13 Online:2025-06-30 Published:2025-07-02

摘要/Abstract

摘要： 采用超高效液相色谱-质谱联用技术结合多元统计方法,系统分析了中华绒螯蟹性早熟个体与正常个体的肌肉与肝胰腺游离氨基酸（FAA）组成差异,并进行了综合生态评价。结果显示：肌肉和肝胰腺组织中均检测到20种FAA,早熟蟹肝胰腺总FAA[（22 210.39±11.59）μg/g]显著高于正常蟹[（17 169.87±11.87）μg/g]（P<0.05）,其中早熟蟹肌肉、肝胰腺中甜味氨基酸总含量均显著高于正常蟹（P<0.05）。早熟蟹鲜味和甜味氨基酸的TAV显著高于正常蟹（P<0.05）,风味表现鲜甜特征,但其综合品质受限于苦味氨基酸的潜在负面影响。研究表明,性早熟可能通过氨基酸代谢途径显著影响中华绒螯蟹机体内FAA组成,且肝胰腺中FAA代谢对性早熟的响应更为敏感。本研究通过风味综合生态分析,为早熟中华绒螯蟹风味品质研究及相关产业应用提供了理论依据。

关键词: 中华绒螯蟹, 肌肉, 肝胰腺, 游离氨基酸

Abstract: In the present study, ultra performance liquid chromatography-mass spectrometry（UPLC-MS）combined with multivariate statistical methods was used to systematically analyze the differences in free amino acid compositions（FAA）of muscle and hepatopancreas between sexually precocious individuals and normal individuals of Eriocheir sinensis, as well as the comprehensive ecological evaluatation. The results showed that 20 FAA were detected in muscle and hepatopancreas tissues respectively, and the total FAAs in hepatopancreas of precocious crab[（22 210.39±11.59）μg/g] were significantly higher than those of normal crab[（17 169.87±11.87）μg/g]（P<0.05）, of which the total contents of sweet amino acids in the muscle and hepatopancreas of precocious crab were significantly higher than those of normal crab（P<0.05）. The TAV of fresh and sweet amino acids in precocious E. sinensis was significantly higher than those of normal crabs（P<0.05）, and the flavor showed fresh and sweet characteristics, but its comprehensive quality was limited by the potential negative effect of bitter amino acids. It was suggested that precocious puberty may significantly affect the FAA composition of E. sinensis through the amino acid metabolic pathway, and FAA metabolism in the hepatopancreas was more sensitive to precocious puberty. The present study provides a theoretical basis for the study of the flavor quality of early-maturing E. sinensis and its application in the industry through the comprehensive ecological analysis of flavor.

Key words: Eriocheir sinensis, muscle, hepatopancreas, free amino acids

中图分类号:

S966.11

缪鈜烨, 刘雅楠, 高金伟, 贾旭颖, 周文礼, 张丹. 性早熟中华绒螯蟹游离氨基酸的呈味分析及生态评价[J]. 天津农学院学报, 2025, 32(3): 12-17.

Miao Hongye, Liu Yanan, Gao Jinwei, Jia Xuying, Zhou Wenli, Zhang Dan. Flavour analysis and ecological evaluation of free amino acids in sexually precocious Eriocheir sinensis[J]. Journal of Tianjin Agricultural University, 2025, 32(3): 12-17.

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http://xuebao.tjau.edu.cn/CN/Y2025/V32/I3/12

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性早熟中华绒螯蟹游离氨基酸的呈味分析及生态评价

Flavour analysis and ecological evaluation of free amino acids in sexually precocious Eriocheir sinensis

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