天津农学院学报 ›› 2023, Vol. 30 ›› Issue (5): 34-39.doi: 10.19640/j.cnki.jtau.2023.05.007

• 研究与简报 • 上一篇    下一篇

三种微藻对不同氮源去除率及CO2固定效率的研究

龙成凤, 张达娟通信作者, 王泽斌, 张树林, 毕相东, 戴伟   

  1. 天津农学院 水产学院 天津市水产生态及养殖重点实验室,天津 300392
  • 收稿日期:2022-09-06 出版日期:2023-10-31 发布日期:2023-12-22
  • 通讯作者: 张达娟(1981—),女,高级实验师,博士,研究方向:养殖水环境调控与修复。E-mail:dajuanzhang@163.com。
  • 作者简介:龙成凤(1991—),女,硕士在读,研究方向为浮游植物分类及生理学研究。E-mail:1035840642@qq.com。
  • 基金资助:
    国家自然科学基金项目(32172978); 中央引导地方(天津)科技发展专项(21ZYCGSN00500); 天津市淡水养殖产业技术体系创新团队-养殖水环境调控岗位(ITTFRS2021000-009); 天津市教委科研计划项目(2020ZD06,2021KJ110); 天津市高等学校创新团队基金项目(TD13-5089); 甘肃省科技计划项目民生科技专项(21CX6NP223)

Study on the removal rate of different nitrogen sources and CO2 fixation efficiency of three microalgae

Long Chengfeng, Zhang DajuanCorresponding Author, Wang Zebin, Zhang Shulin, Bi Xiangdong, Dai Wei   

  1. Tianjin Key Laboratory of Aquatic Ecology and Aquaculture, College of fisheries, Tianjin Agricultural University, Tianjin 300392, China
  • Received:2022-09-06 Online:2023-10-31 Published:2023-12-22

摘要: 水体污染和气候变暖是当今世界面临的重要环境问题,微藻同时具有净化水体及固定CO2的能力,为探讨藻类对三种混合氮源的去除率及对CO2的固定速率,本研究以斜生栅藻(Scenedesmus obliquus)、绿球藻(Chlorococcum sphacosum GD)和普通小球藻(Chlorella vulgaris)为试验对象,接种于由尿素氮、硝态氮和铵态氮(质量浓度为247 mg/L)作为混合氮源的缺碳BG-11培养基中,在通入300 mL/min的空气提供碳源的条件下研究微藻对不同氮源的去除率及CO2的固定效率。结果表明,在混合氮源中,三种微藻对氮源的利用存在差异,铵态氮的吸收利用均受到抑制。斜生栅藻对尿素氮、硝态氮、铵态氮的去除率分别92.90%、79.28%、22.86%,对尿素氮的利用率最高;绿球藻分别为78.00%、80.10%和39.45%,普通小球藻分别为62.00%、81.04%、34.66%,绿球藻和普通小球藻均以对硝态氮的利用率最高。斜生栅藻、绿球藻和普通小球藻CO2最大固定效率分别为0.125、0.142、和0.217 g/(L·d),平均固碳速率分别为0.067、0.097和0.115 g/(L·d),三种微藻固碳效率有所差异,普通小球藻固碳效率最高,其次为绿球藻,斜生栅藻最低。

关键词: 微藻, 尿素氮, 硝态氮, 铵态氮, 去除率, CO2固定效率

Abstract: Water pollution and climate warming are important environmental problems facing the world today. Microalgae have the ability to purify water and fix CO2 at the same time. In order to explore the removal rate of three mixed nitrogen sources and the fixed rate of CO2 by algae, this study took Scenedesmus obliquus, Chlorococcum sphacosum GD and Chlorella vulgaris as experimental objects. Microalgae were inoculated in BG-11 medium with urea nitrogen, nitrate nitrogen and ammonium nitrogen (mass concentration 247 mg/L) as mixed nitrogen sources, and the removal rate of different nitrogen sources and the fixation efficiency of CO2 were studied under the condition of 300 mL /min air supply carbon source. The results showed that there were differences in the utilization of nitrogen sources among the three microalgae, and the absorption and utilization of ammonium nitrogen were inhibited. Urea nitrogen, nitrate nitrogen, ammonium nitrogen removal rate of Scenedesmus obliquus were 92.90%, 79.28% and 22.86% respectively, and utilization of urea nitrogen was the highest. The nitrate utilization rates of Chlorococcum sphacosum GD were 78%, 80.10% and 39.45%, respectively, and those of Chlorella vulgaris were 62.00%, 81.04% and 34.66%, respectively. The maximum CO2 fixation efficiency were 0.125, 0.142, and 0.217 g/(L·d), respectively. The average carbon fixation rate were 0.067, 0.097, and 0.115 g/(L·d), respectively. The carbon fixation efficiency of the Chlorella vulgaris was the highest, followed by the Chlorococcum sphacosum GD, and that of Scenedesmus obliquus was the lowest.

Key words: microalgae, urea nitrogen, nitrate nitrogen, ammonium nitrogen, removal rate, CO2 fixation efficiency

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