天津农学院学报 ›› 2023, Vol. 30 ›› Issue (5): 40-47.doi: 10.19640/j.cnki.jtau.2023.05.008

• 研究与简报 • 上一篇    下一篇

几种环境因子对普通小球藻生长及磷利用率的影响

刘敏, 于建霖, 康会会, 孔庆霞通信作者   

  1. 天津农学院 水产学院,天津 300392
  • 收稿日期:2022-08-08 出版日期:2023-10-31 发布日期:2023-12-22
  • 通讯作者: 孔庆霞(1978—),女,副教授,博士,主要从事水域环境学方面的研究。E-mail:xiaokong3155@163.com。
  • 作者简介:刘敏(1997—),女,硕士在读,主要从事水域环境学研究。E-mail:1140763051@qq.com。
  • 基金资助:
    天津市水产产业技术体系尾水处理岗位(ITTFR2018047); 国家自然科学基金面上项目(31170442)

Effect of several environmental factors on the growth and phosphorus utilization of Chlorella vulgaris

Liu Min, Yu Jianlin, Kang Huihui, Kong QingxiaCorresponding Author   

  1. College of Fisheries, Tianjin Agricultural University, Tianjin 300392, China
  • Received:2022-08-08 Online:2023-10-31 Published:2023-12-22

摘要: 为了探究不同环境因子对普通小球藻生长及磷利用率的影响,本文对不同盐度、藻密度、磷浓度、pH下普通小球藻的生长、磷利用率、叶绿素荧光参数进行探究。结果表明,藻体比生长率、最大光能转化效率(Fv/Fm)、实际光能转化效率(ΦPSⅡ)、潜在活力(Fv/F0)、磷吸附效率随着盐度的增加呈先升后降的趋势,藻体光合活性和磷吸附效率在盐度15时最好。藻体对磷的吸附效率随着藻密度、pH增加呈先升后降的趋势,在藻密度为0.75×106个/mL,pH为7时吸附效率最好。藻体对磷的吸附动力学结果显示,吸附起始阶段1 h内,小球藻对磷的吸附效率可达61%,为快速吸附期。因此,普通小球藻在盐度15、pH为7、藻密度为0.75×106个/mL时生长最快,光合活性最强,且磷吸附效率最高。

关键词: 小球藻, 环境因子, 藻密度, 磷浓度, pH

Abstract: In order to explore the effects of different environmental factors on the growth and phosphorus utilization of Chlorella vulgaris, the growth, phosphorus utilization and chlorophyll fluorescence parameters of C. vulgaris under different salinity, biomass, phosphorus concentration, and pH were investigated in this work. The results showed that the specific growth rate of algae, the maximum light energy conversion efficiency(Fv/Fm), the actual light energy conversion efficiency(ΦPSII), the potential activity(Fv/F0)and the phosphorus adsorption efficiency showed a tendency to rise first and then decrease with the increase of salinity, and the photosynthetic activity and phosphorus adsorption efficiency of the algae were the best at salinity of 15. The adsorption efficiency of phosphorus by algae increased first and then decreased with the increase of algal density and pH, and the adsorption efficiency was best when the algal density was 0.75×106 cells/mL and the pH was 7. Moreover, the adsorption kinetics of algae on phosphorus showed that within 1 h of the initial stage of adsorption, the phosphorus adsorption efficiency of C. vulgaris could reach 61%, which was a rapid adsorption period. Therefore, C. vulgaris has the highest growth rate, the highest photosynthetic activity and the highest phosphorus adsorption efficiency at salinity of 15, pH 7 and algal density of 0.75×106 cells/mL.

Key words: Chlorella vulgaris, environmental factor, algal density, phosphorus concentration, pH

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