中药神农仕复方制剂对猪繁殖与呼吸综合征防治效果研究

doi:10.19640/j.cnki.jtau.2022.02.011

天津农学院学报 ›› 2022, Vol. 29 ›› Issue (2): 53-56.doi: 10.19640/j.cnki.jtau.2022.02.011

中药神农仕复方制剂对猪繁殖与呼吸综合征防治效果研究

任连智¹, 李文忠², 刘野², 杨婧², 程宁², 孙英峰^{2,通信作者}

1.神农世纪生物医学工程研究（天津）有限公司,天津 300385;
2.天津农学院动物科学与动物医学学院,天津 300392

收稿日期:2022-02-23 出版日期:2022-06-30 发布日期:2022-07-05
通讯作者: 孙英峰（1979—）,男,教授,博士,主要从事动物疫病防控研究。E-mail：yfsun2000@163.com。
作者简介:任连智（1954—）,男,高级工程师,博士,主要从事中药抗病毒研究。E-mail：794107248@qq.com。
基金资助:
神农世纪生物医学工程研究（天津）有限公司委托项目（无编号）; 天津市生猪产业技术体系岗位专家项目（ITTPRS2021008）

Prevention and control effect of traditional Chinese medicine on porcine reproductive and respiratory syndrome virus

Ren Lianzhi¹, Li Wenzhong², Liu Ye², Yang Jing², Cheng Ning², Sun Yingfeng^{2,Corresponding Author}

1. Tianjin Shennong Century Biomedical Engineering Research Co., Ltd., Tianjin 300385, China;
2. College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300392, China

Received:2022-02-23 Online:2022-06-30 Published:2022-07-05

摘要/Abstract

摘要： 为探索中药神农仕复方制剂对猪繁殖与呼吸综合征（porcine reproductive and respiratory syndrome,PRRS）的防治效果,本研究选取28日龄健康断奶仔猪40头,随机分为4组,10头/组,分别为空白对照组（negative control group）、攻毒对照组（positive control group）及中药1组（2 kg/t）、中药2组（4 kg/t）组,其中中药1和中药2组分别于攻毒前7 d饲喂中药,攻毒后连续饲喂14 d,每日观察临床症状及测定直肠体温,定期体重测量,试验结束进行病理剖检与肺脏病理组织切片观察、评分。结果显示,相比空白对照组,连续7 d添加中药（2 kg/t、4 kg/t）后可显著提高仔猪日增重（P<0.05）;相比攻毒对照组的存活率为80%,中药组可显著提高仔猪存活率至100%（P<0.01）,且于攻毒后8 d临床症状减轻,体温恢复正常,肺脏未见明显病变,但可见肺泡间隔增厚并伴有轻度炎性细胞浸润,与攻毒对照组差异不显著（P>0.05）;综上研究表明,中药神农仕复方制剂可显著提高日增重,有效改善PRRS病毒（PRRSV）感染猪的临床症状及肺部病理变化。本研究为中药防治PRRS提供了试验依据。

关键词: 中药神农仕复方制剂, PRRS, 日增重, 肺病变

Abstract: ：In order to investigate on the therapeutic effect of Shennongshi Chinese medicine powder on PRRS, forty 4-week-old pigs were randomly divided into four groups: negative control group, positive control group, Chinese medicine prescription group 1（2 kg/t）, Chinese medicine prescription group 2（4 kg/t）. Groups 1 and 2 each were added with herb 7 days before PRRSV challenge, and were continuous fed for 14 days. The clinical symptoms and rectal temperature were observed every day, and the body weight was measured regularly. At the end of the experiment, pathological autopsy and lung samples were collected for pathological tissue section observation and score. The results showed that compared with the negative control group, adding traditional Chinese medicine（2 kg/t, 4 kg/t）for 7 days could significantly increase the daily gain of piglets（P<0.05）; The survival rate of piglets in the traditional Chinese medicine experimental group could be significantly improved to 100%（P<0.01）when compared with the negative control group（80%）; After 8 days of challenge, the clinical symptoms were relieved, the body temperature returned to normal, there was no obvious lesion in the lung, but there was thickening of alveolar septum with mild inflammatory cell infiltration, which was not significantly different from the control group（P>0.05）. In conclusion, this study shows that Shennongshi Chinese medicine powder can significantly improve daily weight gain and effectively improve the clinical symptoms and lung pathological changes of PRRSV infected pigs. This study provides an experimental basis for the prevention and treatment of PRRS with traditional Chinese medicine.

Key words: Shennongshi Chinese medicine powder, PRRSV, daily gain, lung lesion

中图分类号:

S859.3

任连智, 李文忠, 刘野, 杨婧, 程宁, 孙英峰. 中药神农仕复方制剂对猪繁殖与呼吸综合征防治效果研究[J]. 天津农学院学报, 2022, 29(2): 53-56.

Ren Lianzhi, Li Wenzhong, Liu Ye, Yang Jing, Cheng Ning, Sun Yingfeng. Prevention and control effect of traditional Chinese medicine on porcine reproductive and respiratory syndrome virus[J]. Journal of Tianjin Agricultural University, 2022, 29(2): 53-56.

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参考文献

[1] 孙英峰,李志,路超,等. 2014年-2015年天津地区PRRSV分子流行病学调查及流行毒株的分离鉴定[J].动物医学进展,2016,37(10):11-15.
[2] 郭宝清,陈章水,刘文兴,等. 从疑似PRRS流产胎儿分离PRRSV的研究[J]. 中国畜禽传染病,1996(2):3-7.
[3] 石青青,马吉飞,孙英峰. 我国猪繁殖与呼吸障碍综合征类NADC30毒株流行现状[J]. 中国猪业,2018,13(1):42-45.
[4] 魏彤,冯鹏. 中草药的功能及其在饲料中的应用[J]. 当代畜禽养殖业,2017(10):47-48.
[5] 潘英芳. 中草药在兽医临床治疗中的应用[J]. 兽医导刊,2021(19):108-109.
[6] 李锐,肖燕,和心依,等. 中药姜黄化学成分、生物活性及体内代谢研究进展[J]. 西华大学学报(自然科学版),2013,32(3):98-104.
[7] 张丽媛,魏曼琳,吴白乙拉,等. 葫芦巴的生物学功能及其在动物生产中的应用[J]. 饲料研究,2021,44(15):140-143.
[8] 叶慧鸿. 浅谈黄芪多糖在动物生产上的应用[J]. 山东畜牧兽医,2016,37(1):53-54.
[9] HALBUR P G,PAUL P S,FREY M L,et al.Comparison of the pathogenicity of two US porcine reproductive and respiratory syndrome virus isolates with that of the Lelystad virus[J]. Veterinary Pathology,1995,32(6):648-660.
[10] FRYDAS I S,VERBEECK M,CAO J,et al.Replication characteristics of porcine reproductive and respiratory syndrome virus(PRRSV)European subtype 1(Lelystad)and subtype 3(Lena)strains in nasal mucosa and cells of the monocrytic lineage: indications for the use of new receptors of PRRSV(Lean)[J]. Vet Res,2013,44(1):73-86.
[11] 孙英峰. 天津地区PRRSV类NADC30毒株的监测、分离毒株的基因组特征与致病性分析[D]. 北京:中国农业大学,2017.
[12] 任秀伟. 浅析中药抗病毒在兽医临床的应用与作用机理[J]. 农业开发与装备,2019(12):129-132.
[13] 钱海霞. 抗病毒中药在兽医临床的应用与作用机理[J]. 今日畜牧兽医,2021,37(8):82.
[14] 李淑焕,薄永恒. 兽用抗病毒中药复方制剂抗炎机理的研究[J]. 山东畜牧兽医,2019,40(6):5-7.
[15] 田朝晖,张彪. 板蓝根、黄芪和青蒿提取物抗PRRSV作用比较[J]. 中国饲料,2016(17):16-19.
[16] 李雪,张会从,李勉,等. 三种中药复方体外抗高致病性蓝耳病毒的效果观察[J]. 黑龙江畜牧兽医,2012(5):132-134.
[17] 李桂叶. 加味板蓝根散防治蓝耳病的效果[J]. 养殖与饲料,2018(2):34-35.

中药神农仕复方制剂对猪繁殖与呼吸综合征防治效果研究

Prevention and control effect of traditional Chinese medicine on porcine reproductive and respiratory syndrome virus

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