纤细裸藻共栖细菌的分离鉴定及其对藻细胞生长的影响

doi:10.19640/j.cnki.jtau.2022.02.012

Journal of Tianjin Agricultural University ›› 2022, Vol. 29 ›› Issue (2): 57-62.doi: 10.19640/j.cnki.jtau.2022.02.012

• Researches and Scientific Notes • Previous Articles Next Articles

Isolation of symbiotic bacteria of Euglena gracilis and effects on the growth of algal cells

Wang Xiaoyu¹, Ren Shutian², Wu Yating², Wu Zun², Gao Haofeng², Shao Peng^{2,Corresponding Author}

1. Tianjin Fisheries Research Institute, Tianjin 300221, China;
2. College of Fisheries, Tianjin Agricultural University, Tianjin 300392, China

Received:2022-05-01 Online:2022-06-30 Published:2022-07-05

Abstract

Abstract: The symbiotic bacteria of Euglenum gracilis were isolated, and the influence of symbiotic bacteria on the growth of Euglenum gracilis was studied. The symbiotic bacteria were isolated by plate scribing and identified by 16S rDNA gene sequencing. Five species of symbiotic bacteria were obtained, E1-Paraburkholderia fungorum, E3-Methylobacterium radiotolerans, E5-Sphingomonas dokdonensis, E6-Ralstonia insidiosa and ET2-Leifsonia soli, which belong to α-Proteobacteria, β-Proteobacteria and Actinobacteria, respectively. The results demonstrated that E1, E3 and E5 could enhance the growth of Euglenum gracilis by measuring the cell density and chlorophyll content of algae in co-culture systems of bacteria and algae, and that E5 had the best effect. The promoting effect of Euglena gracilis was best when initial inoculation concentration of E5 bacterium was 10⁷cfu/mL and the initial cell density of Euglena gracilis was 1×10⁵ cells/mL.

Key words: Euglena gracilis, symbiotic bacteria, co-culture of bacteria and algae

CLC Number:

S917.1

Wang Xiaoyu, Ren Shutian, Wu Yating, Wu Zun, Gao Haofeng, Shao Peng. Isolation of symbiotic bacteria of Euglena gracilis and effects on the growth of algal cells[J]. Journal of Tianjin Agricultural University, 2022, 29(2): 57-62.

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: http://xuebao.tjau.edu.cn/EN/10.19640/j.cnki.jtau.2022.02.012

http://xuebao.tjau.edu.cn/EN/Y2022/V29/I2/57

References

[1] SARMENTO H,GASOL J M.Use of phytoplankton-derived dissolved organic carbon by different types of bacterioplankton[J]. Environmental Microbiology,2012,14(9):2348-2360.
[2] 高亚辉. 海洋微藻分类生态及生物活性物质研究[J]. 厦门大学学报(自然科学版),2001,40(2):566-573.
[3] FUJITA T,AOYAGI H,OGBONNA J C,et al.Effect of mixed organic substrate on α-tocopherol production by Euglena gracilisin photoheterotrophic culture[J]. Applied Microbiology & Biotechnology,2008,79(3):371-378.
[4] NOVAK M,VETVICKA V.β-Glucans,history,and the present:immunomodulatory aspects and mechanisms of action[J]. Journal of Immunotoxicology,2008,20(5):47-57.
[5] IRINA M,IVANOVA,SERGEY A,et a1. Fluorescent mannosides serve as acceptor substrates for glycosyltransferase and sagar-1-phosphate transferase activities in Euglena gracilis membranes[J]. Carbohydrate Research,2017,483:26-38.
[6] 张文慧,窦勇,高金伟,等. 纤细裸藻对5种抗生素的胁迫响应[J]. 水生态学杂志,2017,38(6):48-56.
[7] KAZAMIA E,CZESNICK H,NGUYEN T V,et al.Mutualistic interactions between vitamin B12-dependent algae and heterotrophic bacteria exhibit regulation[J]. Environmental Microbiology,2012,14(483):1466-1476.
[8] FALKOWSKI P G,FENCHEL T,DELONG E F.The microbial enginesthat drive earth’s biogeochemical cycles[J]. Science,2008,320(5879):1034-1039.
[9] CHEVANTON I,GARNIER M,BOUGARAN G,et al.Screening and selection of growth-promoting bacteria for Dunaliella cultures[J]. Algal Research,2013,2(3):212-222.
[10] CHO D H,RAMANAN R,HEO J,et al.Enhancing microalgal biomass productivity by engineering a microalgal- bacterial community[J]. Bioresource Technology,2015,175(7):578-585.
[11] 黄振华,陈星星,柯爱英,等. 2种海洋饵料微藻藻际环境的细菌多样性研究[J]. 水生态学杂志,2018,39(1):44-49.
[12] STEVEN S H,STOBNER M,SIMON M,et al.Phylogeny of Proteobacteria and Bacteroidetes from oxic habitats of a tidal flat ecosystem[J]. FEMS Microbiology Ecology,2005,54(7):351-365.
[13] KIM S J,MOON J Y,LIM J M,et al.Sphingomonas aerophila sp. nov. and Sphingomonas naasensis sp. nov. isolated from air and soil,respectively[J]. International Journal of Systematic & Evolutionary Microbiology,2014,64(3):926-932.
[14] HUY H,JIN L,LEE K C,et al.Sphingomonas daechungensis sp. nov.,isolated from sediment of a eutrophic reservoir[J]. Int J Syst Evol Microbiol,2014,64(8):1412-1418.
[15] PARK H K,HAN J H,KIM T S,et al.Sphingomonas aeria sp. nov. from indoor air of a pharmaceutical environment[J]. Antonie van Leeuwenhoek,2015,107(1):47-53.
[16] KIM Y J,LIM J,SUKWEENADHI J,et al.Genomic characterization of a newly isolated Rhizobacteria Sphingomonas panacis reveals plant growth promoting effect to rice[J]. Biotechnology and Bioprocess Engineering,2019,24(2):119-125.
[17] JEON M S,OH J J,Kim J Y,et al.Enhancement of growth and paramylon production of Euglena gracilis by co-cultivation with Pseudoalteromonas sp. MEBiC 03485[J]. Bioresource Technology,2019,288(9):1-8.
[18] LEE C,JEON M S,KIM J Y,et al.Effects of an auxin-producing symbiotic bacterium on cell growth of the microalga Haematococcus pluvialis:Elevation of cell density and prolongation of exponential stage[J]. Algal Research,2019,41(8):1-8.

Isolation of symbiotic bacteria of Euglena gracilis and effects on the growth of algal cells

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 8

Recommended Articles

Metrics

Comments

[1]	Chen Xuan, Zhang Dajuan, Zhang Shulin, Wang Zebin, Dai Wei, Bi Xiangdong. The competition and allelopathy of Microcystis aeruginosa with Cyanoidetes longissima and Scenedesmus quadricauda [J]. Journal of Tianjin Agricultural University, 2023, 30(4): 32-38.
[2]	Ge Lunhua, Jiang Boyun, Sun Jingfeng, Lü Aijun, Hu Xiucai, Chen Limei. Effects of different DNA extraction methods on structural analysis of intestinal microbiota from Cynoglossus semilaevis based on high-throughput sequencing [J]. Journal of Tianjin Agricultural University, 2022, 29(3): 29-35.
[3]	Gu Xueqin, Wang Qingkui, Wang Yang. Research progress on bacterial community structure in recirculating aquaculture system for marine fish [J]. Journal of Tianjin Agricultural University, 2022, 29(2): 76-84.
[4]	YE Ming-hao, HU Xiu-cai, LÜ Ai-jun, SUN Jing-feng, LIU Xiao-xue, BAI Jie. Isolation, identification and antibiotic sensitivity of the pathogenic Shewanella putrefaciens from the grass carp Ctenopharyngodon idellus [J]. Journal of Tianjin Agricultural University, 2018, 25(4): 52-57.
[5]	ZHAN Hai, WANG Guang-jun, CHEN Cheng-xun. Identification and Denitrification Ability of an Aerobic Denitrifier [J]. Journal of Tianjin Agricultural University, 2017, 24(2): 44-48.
[6]	HU Xuan, SUN Jing-feng, CHEN Cheng-xun, XING Ke-zhi. Isolation and Identification of Pathogen of Ascites Disease from Cynoglossus semilaevis Günther and Its Antibiotic Sensitivity Analysis [J]. Journal of Tianjin Agricultural University, 2014, 21(3): 12-16.
[7]	SUN Cheng-bo, MIAO Jian-sheng, HAN Zhi-hui, ZHAO Dong-yan, LI Jian-guo, RONG Hai-yan. Phytoplankton Analysis Based on Multiple Bio-treatment System [J]. Journal of Tianjin Agricultural University, 2012, 19(3): 52-57.
[8]	PENG Hui-hui, ZHANG Kai, CHEN Cheng-xun, XIE Jun, LI Zhi-fei, YU Er-meng, LIU Long-fei, GONG Wang-bao, TIAN Jing-jing, WANG Guang-jun. Effects of carp（Cyprinus carpio）on phytoplankton and environmental factors in paddy fields [J]. Journal of Tianjin Agricultural University, 2020, 27(1): 37-43.