3种非特异性免疫相关基因在点带石斑鱼组织中的mRNA表达分析

Journal of Tianjin Agricultural University ›› 2015, Vol. 22 ›› Issue (1): 1-5.

• Orginal Article • Next Articles

mRNA Expression of Three Non-specific Immune Genes in Tissues of Epinephelus malabaricus

LI Xiao-meng, DAI Wei, FANG Zhen-zhen, XING Ke-zhi^*, WANG Qing-kui, CHEN Cheng-xun

College of Fisheries, Tianjin Agricultural University, Tianjin 300384, China

Received:2014-11-04 Published:2015-03-31

Abstract

Abstract: ：Using β-actin mRNA expression quantity as an internal reference, the mRNA expression differences of three non-specific immune genes, c-type lysozyme (CTL), interleukin 8 (IL8) and toll-like receptor 5 (TLR5), were analyzed in head kidney, heart, liver, spleen, stomach, intestine and gill of Epinephelus malabaricus with semi-quantitative RT-PCR technique. The results show that mRNA expressions of three genes were observed in all of the above tissues. The highest mRNA expression of CTL gene was detected in spleen while the highest mRNA expression for both IL8 and TLR5 genes was head kidney. The tissues with the lowest mRNA expressions for CTL, IL8 and TLR5 genes were intestine, gill and heart, respectively.

Key words: Epinephelus malabaricus, semi-quantitative RT-PCR, gene expression

CLC Number:

S965.334

LI Xiao-meng, DAI Wei, FANG Zhen-zhen, XING Ke-zhi, WANG Qing-kui, CHEN Cheng-xun. mRNA Expression of Three Non-specific Immune Genes in Tissues of Epinephelus malabaricus[J]. Journal of Tianjin Agricultural University, 2015, 22(1): 1-5.

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mRNA Expression of Three Non-specific Immune Genes in Tissues of Epinephelus malabaricus

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