Abstract: Amino acid permeases（AAPs）play an important regulatory role in the transport of amino acids in plants. In order to further explore the biological function of maize AAP gene, in this study, the AAP gene（ZmAPP）was cloned from corn ‘B73’, and its sequence was analyzed by bioinformatics. The green fluorescent protein EGFP expression vector PCAMBIA1300- ZmAAP-EGFP was constructed. The onion epidermis was transformed by agrobacterium mediated method, and gene expression was observed by using fluorescence microscopy. The results showed thatthe total length of maize AAP gene cDNA sequence was 1 407 bp, encoding 468 amino acids, the theoretical isoelectric point（pI）was 8.72, the instability coefficient was 36.93（<40）, and the total average hydrophilicity was 0.487, which belonged to the alkaline stable hydrophobic protein. Multiple sequence alignment and phylogenetic analysis showed that ZmAAP and barley AAP had the highest similarity at amino acid level, and subcellular localization results showed that ZmAAP was localized in cell membrane. This study serves as a basis for further research on maize AAP gene function and amino acid transport mechanism.

Key words: maize, ZmAAP gene, bioinformatics analysis, subcellular localization