Abstract: In order to achieve high-density cultivation, mycelial biomass as the primary indicator, combined with density, diameter, uniformity of mycelial pellets, and re-cultured germination time on plates as secondary indicators, the medium and fermentation conditions of Pleurotus eryngii for shake-flask liquid spawn were optimized by single-factor and orthogonal experiments. The results showed that the optimal fermentation conditions were:α-amylase pretreated corn flour 22 g, wheat bran 11 g, potassium dihydrogen phosphate 2 g, and magnesium sulfate 1.1 g/L, pH 5.0, rotational speed 180 r/min, liquid volume 100 mL/250 mL conical flask, inoculation age 5.5 days, and inoculation volume 8%. After 6 days of cultivation, the biomass of Pleurotus eryngii liquid spawn reached 37.235 g/L, which improved by 23.56 times compared with the initial conditions, germination time shortened to 3.7 hours, mycelial pellet density of 324 pellets/mL, and mycelial pellet diameter of 1.45 mm/pellet.

Key words: Pleurotus eryngii, liquid spawn, high-density cultivation, optimization of fermentation condition optimization