EpCSP基因生物信息学分析及亚细胞定位

doi:10.19640/j.cnki.jtau.2025.04.003

Journal of Tianjin Agricultural University ›› 2025, Vol. 32 ›› Issue (4): 18-25.doi: 10.19640/j.cnki.jtau.2025.04.003

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Bioinformatics analysis and subcellular localization of EpCSP gene

Zhong Yinglan, Zhang Fulong, Li Xinyu, Du Jin, Huang Haidong, Wu Jiang^{Corresponding Author}

College of Agronomy and Resource Environment, Tianjin Agricultural University, Tianjin 300392, China

Received:2024-01-08 Published:2025-09-02

Abstract

Abstract: EpCSP gene is a cold shock protein gene cloned from Exiguobacterium profundum, and bioinformatics analysis suggests that the protein encoded by this gene may be located in the nucleus or cytoplasm matrix. To further investigate the function of the gene, the CDS region of the EpCSP gene was amplified using PCR technology and cloned onto the expression vector pCAMBIA1300-EGFP-MCS to construct a recombinant plasmid for transient expression of EpCSP protein. After PCR identification, the recombinant plasmid was transformed into Agrobacterium tumefaciens LBA4404 using electric shock transformation method and transformed into onion epidermal cells. Its subcellular localization was determined by fluorescence microscopy observation. The results showed that the inserted fragment was completely consistent with the expected sequence and formed a complete gene expression system with the vector. Subcellular localization results showed that the EpCSP protein was distributed in the cytoplasm matrix.

Key words: EpCsp gene, bioinformatics, onion epidermal cells, subcellular localization

CLC Number:

Q789

Zhong Yinglan, Zhang Fulong, Li Xinyu, Du Jin, Huang Haidong, Wu Jiang. Bioinformatics analysis and subcellular localization of EpCSP gene[J]. Journal of Tianjin Agricultural University, 2025, 32(4): 18-25.

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Bioinformatics analysis and subcellular localization of EpCSP gene

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