响应面法优化牛骨肉蛋白液酶解工艺条件

Journal of Tianjin Agricultural University ›› 2017, Vol. 24 ›› Issue (3): 73-78.

• Researches and Scientific Notes • Previous Articles Next Articles

Optimization of Hydrolysis Conditions of Bovine Bone Protein Extract by Response Surface Methodology

FAN Xiao-pan, QIU Hong-bo, MA Li-zhen^{Corresponding Author}

Tianjin Engineering and Technology Research Center of Agricultural Products Processing, College of Food Science and Biotechnology, Tianjin Agricultural University, Tianjin 300384, China

Received:2016-11-29 Online:2017-09-30 Published:2019-10-15

Abstract

Abstract: In this study, bovine bone were chosen as raw material (2.15% of crude protein content). Study on the influence of technological condition of enzymolysis by two kinds of protease (flavourzyme and protamex). The research results showed that the optimal hydrolysis conditions of flavourzyme were as follows: temperature 45 ℃, hydrolysis time 4.3 h and the enzyme addition 50 LAPU/g protein. Under this condition, the degree of hydrolysis was 15.88%. The optimum conditions for protamex extraction were found to be hydrolysis at 45 ℃ for 4.5 h with an enzyme/substrate ratio of 1 500 U/g, the degree of hydrolysis under this conditions was 12.78%.

Key words: bovine bone, enzymatic hydrolysis, flavourzyme, protamex, degree of hydrolysis

CLC Number:

TS251.94

FAN Xiao-pan, QIU Hong-bo, MA Li-zhen. Optimization of Hydrolysis Conditions of Bovine Bone Protein Extract by Response Surface Methodology[J]. Journal of Tianjin Agricultural University, 2017, 24(3): 73-78.

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Optimization of Hydrolysis Conditions of Bovine Bone Protein Extract by Response Surface Methodology

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