酵母双杂交方法对牛CMYA1中Xin Repeat结构域的鉴定

Journal of Tianjin Agricultural University ›› 2017, Vol. 24 ›› Issue (1): 28-33.

• Researches and Scientific Notes • Previous Articles Next Articles

Identification of Xin Repeat Domain of CMYA1 in Bovine by Yeast Two-Hybrid

WANG Ting, YUE Ying-wei, YANG Shu-ping, JIN Cong-fei, XIN Xiang-bo, ZHANG Xiao-juan, GUO Hong^{Corresponding Author}

College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China

Received:2016-05-05 Online:2017-03-31 Published:2019-10-15

Abstract

Abstract: CMYA1 is a muscle development related gene which expression product contains a number of repeat sequences composed of 16 amino acids, named Xin Repeat. To determine the CMYA1 domain of protein interaction, this study used Xin Repeats as a predicted domain of CMYA1. According to different number of Xin Repeats, we divided CMYA1 into different fragments and cloned into expression vectors and transformed into Yeast strain.As result, we obtained 6 fragments of CMYA1 fusion vectors for Yeast two-Hybrid as baits. The minimal unit of protein interacted domain was four Xin Repeats. This study revealed the structure of CMYA1 and the function of Xin Repeats, and provided a theoretical basis of molecular mechanism on bovine muscle growth and development.

Key words: CMYA1, Xin Repeat, domain, Yeast two-Hybrid

CLC Number:

S813.1

WANG Ting, YUE Ying-wei, YANG Shu-ping, JIN Cong-fei, XIN Xiang-bo, ZHANG Xiao-juan, GUO Hong. Identification of Xin Repeat Domain of CMYA1 in Bovine by Yeast Two-Hybrid[J]. Journal of Tianjin Agricultural University, 2017, 24(1): 28-33.

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: http://xuebao.tjau.edu.cn/EN/

http://xuebao.tjau.edu.cn/EN/Y2017/V24/I1/28

References

[1] Wang D Z,Hu X,Lin J L,et al.Differential display of mRNAs from the atrioventricular region of developing chicken hearts at stages 15 and 21[J]. Front Biosci,1996,1:a1-15.
[2] Wang D Z,Reiter R S,Lin J L,et al.Requirement of a novel gene,Xin,in cardiac morphogenesis[J]. Development,1999,126(6):1281-1294.
[3] Sinn H W,Balsamo J,Lillien J,et al.Localization of the novel in protein to the adherens junction complex in cardiac and skeletal muscle during development[J]. Dev Dynam,2002,225(1):1-13.
[4] Pacholsky D,Vakeel P,Himmel M,et al.Xin Repeats define a novel actin-binding motif[J]. J Cell Sci,2004,117(11):5257-5268.
[5] Eulitz S,Sauer F,Pelissier M C,et al.Identification of XIN-repeat proteins as novel ligands of the SH3 domains of nebulin and nebulette and analysis of their interaction during myofibril formation and remodeling[J]. MolBiol Cell,2013,24(20):3215-3226.
[6] Lin J J C,Gustafson-Wagner E A,Sinn H W,et al. Structure,ex-pression,and function of a novel intercalated disc protein,XIN[J]. J Med Sci,2005,25(5):215-222.
[7] Choi S,Gustafson W E A,Wang Q,et al. The intercalated disc protein,mXINa,is capable of interacting with b-catenin and bundling actin filaments[J]. J BiolChem,2007,282(49):36024-36036.
[8] Hawke T J,Atkinson D J,Kanatous S B,et al.XIN,an actin binding protein,is expressed within muscle satellite cells and newly regenerated skeletal muscle fibers[J]. Am J Physiol Cell Physiol,2007,293(5):1636-1644.
[9] Molt S,Buhrdel J B,Yakovlev S,et al.Aciculin interacts with filamin C and XIN and is essential for myofibril assembly,remodeling and maintenance[J]. J Cell Science,2014,127(16):3578-3592.
[10] Traub P,Bauer C,Hartig R,et al.Colocalization of single ribosomes with intermediate filaments in puromycin- treated and serum-starved mouse embryo fibroblasts[J]. BiolCell,1998,90(4):319-337.
[11] Jaqaman K,Grinstein S.Regulation from within:the cytoskeleton in transmembrane signaling[J]. Trends Cell Biol,2012,22(10):515-526.
[12] Hershey J W,Asano K,Naranda T,et al.Conservation and diversity in the structure of translation initiation factor EIF3 from humans and yeast[J]. Biochimie,1996,78(11-12):903-907.
[13] Loughlin L O,Lehr L,Havaranis A,et al.Encapsulation of “Core”eIF3,Regulatory components of elF3 and mRNA into liposomes,and their subsequent uptake into myogenic cells in culture[J]. J Cell Biol,1981,90(1):160-168.

Identification of Xin Repeat Domain of CMYA1 in Bovine by Yeast Two-Hybrid

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 0

Recommended Articles

Metrics

Comments