Abstract: By comparing the mycelium growth, mycelium pellets number and mycelium dry weight of Weimo, the most suitable initial fermentation medium for Weimo was selected from the four types of fermentation culture medium. Then L₉(3⁴) orthogonal experiment design was employed to optimize the medium. The determined fermentation medium as the following: glucose 15 g/L, lactose 15 g/L, yeast paste 4 g/L and the nature pH value. The dry mycelium weight of Weimo can reach to 18.26 g/L with the optimum culture medium.

Key words: Weimo, orthogonal experiment, dry mycelium weight, mycelium pellet